甘蔗内生毛霉菌的拮抗和分子特征

IF 1.4 4区 生物学 Q3 BIOLOGY Biologia Pub Date : 2024-09-19 DOI:10.1007/s11756-024-01777-4
Moisés Rodrigues Silva, Eder Marques, Marcos Gomes da Cunha
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引用次数: 0

摘要

毛霉菌属在全世界被用于控制真菌疾病和促进植物生长。它是许多研究的主题,也是大多数生物防治剂商业配方的组成部分。在巴西,它被用于大约 78 种注册商业产品中。这项工作旨在通过配对培养和产生挥发性和非挥发性代谢物的生物测定,从甘蔗中分离和评估毛霉内生菌的体外拮抗作用,此外还根据分子标记的部分测序对其进行分子鉴定。在针对 Fusarium verticillioides、Phaeocytostroma sacchari、Thielaviopsis sp.和 Colletotrichum falcatum 的配对培养试验中,菌丝生长抑制率超过 76.27%。在评估挥发性和非挥发性代谢物产生情况的试验中,分离物的抑制率分别为 0 至 50.19%和 0 至 35.69%。根据所进行的分子系统发育,对遗传标记的联合分析表明,这 7 个分离株属于非洲毛霉,鉴于观察到其作为甘蔗病原体拮抗剂的巨大潜力,将通过体内病害控制试验对其进行评估,并测试其对该植物生长的促进作用。
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Antagonistic and molecular characterization of endophytic Trichoderma from sugarcane

The genus Trichoderma is used worldwide to control fungal diseases and promote the growth of plants. It is the subject of many studies and forms part of most commercial formulations with biological control agents. In Brazil, it is used in approximately 78 registered commercial products. This work aims to isolate and evaluate the in vitro antagonism of Trichoderma endophytes from sugarcane, through pairing cultures and bioassays of the production of volatile and non-volatile metabolites, in addition to carrying out its molecular identification based on partial sequencing of molecular markers. In the paired culture assay against Fusarium verticillioides, Phaeocytostroma sacchari, Thielaviopsis sp. and Colletotrichum falcatum, mycelial growth inhibition rates were above 76.27%. In tests evaluating the production of volatile and non-volatile metabolites, the isolates promoted inhibitions that varied between 0 and 50.19% and 0 and 35.69%, respectively. According to the molecular phylogeny carried out, the concatenated analysis of the genetic markers revealed that the seven isolates belong to the species Trichoderma afroharzianum and, given the great potential as antagonists observed for sugarcane pathogens, they will be evaluated through in vivo disease control trials and tested for the growth promotion of this plant.

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来源期刊
Biologia
Biologia 生物-生物学
CiteScore
3.30
自引率
6.70%
发文量
290
审稿时长
6 months
期刊介绍: Established in 1946, Biologia publishes high-quality research papers in the fields of microbial, plant and animal sciences. Microbial sciences papers span all aspects of Bacteria, Archaea and microbial Eucarya including biochemistry, cellular and molecular biology, genomics, proteomics and bioinformatics. Plant sciences topics include fundamental research in taxonomy, geobotany, genetics and all fields of experimental botany including cellular, whole-plant and community physiology. Zoology coverage includes animal systematics and taxonomy, morphology, ecology and physiology from cellular to molecular level.
期刊最新文献
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