斯瓦尔巴特(北纬 79 度)Ny Ålesund 永久冻土活动层中细菌 MAGs 的特定深度分布情况

IF 3.3 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Systematic and applied microbiology Pub Date : 2024-08-28 DOI:10.1016/j.syapm.2024.126544
Katie Sipes , Joy Buongiorno , Andrew D. Steen , Andrey A. Abramov , Chukwufumnanya Abuah , Samantha L. Peters , Richard J. Gianonne , Robert L. Hettich , Julia Boike , Sarahi L. Garcia , Tatiana A. Vishnivetskaya , Karen G. Lloyd
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引用次数: 0

摘要

北极土壤微生物群落可能会随着温度的升高和气候变化带来的水供应量的增加而发生变化。我们考察了斯瓦尔巴群岛Ny Ålesund的Bayelva监测站2年(2018-2019年)来受永久冻土影响的土壤上层80厘米处的温度和体积液态水含量(VWC)。我们的研究表明,VWC随深度增加而增加,而原地温度在垂直方向较为稳定,季节性温度范围为-5°C至5°C。在2018年4月冷冻时采集的2-4厘米垂直分辨率和2019年9月解冻时采集的10厘米垂直分辨率的原核生物元基因组(MAGs)均已获得。最丰富的 MAGs 是酸性菌群(Acidobacteriota)、放线菌群(Actinomycetota)和绿僵菌群(Chloroflexota)。放线菌群(Actinomycetota)和绿菌群(Chloroflexota)随着深度的增加而增加,而酸性细菌群(Acidobacteriota)类中的Thermoanaerobaculia Gp7-AA8、Blastocatellia UBA7656和Vicinamibacteria Vicinamibacterales则分别出现在6厘米以上、6厘米以下和20厘米以下。所有 MAGs 都有多种碳降解基因,放线菌属(Actinomycetota)和绿僵菌属(Chloroflexota)有自养基因。编码β-葡萄糖苷酶、N-乙酰-β-D-葡萄糖苷酶和木糖苷酶的基因随着深度的增加而增加,表明随着 VWC 越高,有机物降解的潜力越大。酸性菌群主要分布在顶部 6 厘米处,其类别随深度而分离,而放线菌群和绿藻菌群主要分布在 6 厘米以下。这表明酸性菌群适应地表较低的 VWC,而放线菌群和绿僵菌群则在 6 厘米以下持续存在,具有较高的 VWC。这表明,在北极矿质土壤的微生物气候变化响应中,VWC 可能与温度一样重要。在此,我们描述了酸细菌群(Onstottus arcticum、Onstottus frigus 和 Gilichinskyi gelida)和放线菌群(Mayfieldus profundus)中基于 MAG 的 Seqcode 类型物种。
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Depth-specific distribution of bacterial MAGs in permafrost active layer in Ny Ålesund, Svalbard (79°N)

Arctic soil microbial communities may shift with increasing temperatures and water availability from climate change. We examined temperature and volumetric liquid water content (VWC) in the upper 80 cm of permafrost-affected soil over 2 years (2018–2019) at the Bayelva monitoring station, Ny Ålesund, Svalbard. We show VWC increases with depth, whereas in situ temperature is more stable vertically, ranging from −5°C to 5 °C seasonally. Prokaryotic metagenome-assembled genomes (MAGs) were obtained at 2–4 cm vertical resolution collected while frozen in April 2018 and at 10 cm vertical resolution collected while thawed in September 2019. The most abundant MAGs were Acidobacteriota, Actinomycetota, and Chloroflexota. Actinomycetota and Chloroflexota increase with depth, while Acidobacteriota classes Thermoanaerobaculia Gp7-AA8, Blastocatellia UBA7656, and Vicinamibacteria Vicinamibacterales are found above 6 cm, below 6 cm, and below 20 cm, respectively. All MAGs have diverse carbon-degrading genes, and Actinomycetota and Chloroflexota have autotrophic genes. Genes encoding β -glucosidase, N-acetyl-β-D-glucosaminidase, and xylosidase increase with depth, indicating a greater potential for organic matter degradation with higher VWC. Acidobacteriota dominate the top 6 cm with their classes segregating by depth, whereas Actinomycetota and Chloroflexota dominate below ∼6 cm. This suggests that Acidobacteriota classes adapt to lower VWC at the surface, while Actinomycetota and Chloroflexota persist below 6 cm with higher VWC. This indicates that VWC may be as important as temperature in microbial climate change responses in Arctic mineral soils. Here we describe MAG-based Seqcode type species in the Acidobacteriota, Onstottus arcticum, Onstottus frigus, and Gilichinskyi gelida and in the Actinobacteriota, Mayfieldus profundus.

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来源期刊
Systematic and applied microbiology
Systematic and applied microbiology 生物-生物工程与应用微生物
CiteScore
7.50
自引率
5.90%
发文量
57
审稿时长
22 days
期刊介绍: Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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