局部中/高危和转移性前列腺癌循环肿瘤细胞的多标记分析

IF 4.2 3区 医学 Q2 ONCOLOGY Clinical & Experimental Metastasis Pub Date : 2024-09-21 DOI:10.1007/s10585-024-10313-2
Eva Welsch, Lilli Bonstingl, Barbara Holzer, Eva Schuster, Esther Weiß, Alexandru-Teodor Zaharie, Michael Krainer, Michael B Fischer, Amin El-Heliebi, Robert Zeillinger, Eva Obermayr
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引用次数: 0

摘要

循环肿瘤细胞(CTCs)是转移性前列腺癌(PrC)的公认预后标志物,但在局部高危疾病中却很少受到关注。研究人员在基线期、放疗后和随访期间从早期、中期和高危前列腺癌患者(15 人)以及转移性前列腺癌患者(23 人)身上采集了外周血。采用微流控 Parsortix® 技术富集 CTC。通过 qPCR 和 RNA 原位杂交 (ISH) 对 CTC 相关标记进行量化。阳性率及与临床参数的关系采用 McNemar 检验、Fisher Exact 检验或对数秩检验进行评估。两组患者的总体阳性率都很高(基线时87.0%为转移癌,66.7%为早期癌)。qPCR 和 RNA ISH 的一致性很高。在转移性 PrC 患者中,PSA 和 PSMA 是缩短总生存期的预后指标。在早期 PrC 患者中,从放疗前到放疗后,每份血液样本中的阳性转录物有所增加,而在随访期间,阳性标记物有所减少。使用所研究的 qPCR 标记小组进行 CTC 分析,即使在局部疾病中也能实现 PrC 患者样本的高检出率。RNA ISH 具有在单细胞水平确认这些标记物的优势。我们的 CTC 方法采用了与临床相关的标志物 PSMA,可用于诊断目的,筛查可从 PSMA 引导的 PET-CT 或 PSMA 靶向治疗中获益的患者。
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Multi-marker analysis of circulating tumor cells in localized intermediate/high-risk and metastatic prostate cancer.

Circulating tumor cells (CTCs) are an established prognostic marker in metastatic prostate cancer (PrC) but have received little attention in localized high-risk disease. Peripheral blood was obtained from patients with early intermediate and high-risk PrC (n = 15) at baseline, after radiotherapy, and during follow-up, as well as from metastatic PrC patients (n = 23). CTCs were enriched using the microfluidic Parsortix® technology. CTC-related marker were quantified with qPCR and RNA in-situ hybridization (ISH). Positivity and associations to clinical parameters were assessed using McNemar test, Fisher Exact test or log-rank test. The overall positivity was high in both cohorts (87.0% metastatic vs. 66.7% early at baseline). A high concordance of qPCR and RNA ISH was achieved. In metastatic PrC, PSA and PSMA were prognostic for shorter overall survival. In early PrC patients, an increase of positive transcripts per blood sample was observed from before to after radiation therapy, while a decrease of positive markers was observed during follow-up. CTC analysis using the investigated qPCR marker panel serves as tool for achieving high detection rates of PrC patient samples even in localized disease. RNA ISH offers the advantage of confirming these markers at the single cell level. Employing the clinically relevant marker PSMA, our CTC approach can be used for diagnostic purposes to screen patients profiting from PSMA-directed PET-CT or PSMA-targeted therapy.

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来源期刊
CiteScore
7.80
自引率
5.00%
发文量
55
审稿时长
12 months
期刊介绍: The Journal''s scope encompasses all aspects of metastasis research, whether laboratory-based, experimental or clinical and therapeutic. It covers such areas as molecular biology, pharmacology, tumor biology, and clinical cancer treatment (with all its subdivisions of surgery, chemotherapy and radio-therapy as well as pathology and epidemiology) insofar as these disciplines are concerned with the Journal''s core subject of metastasis formation, prevention and treatment.
期刊最新文献
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