低温电子断层扫描揭示的 CTPS 纤维网络结构。

IF 3.3 3区 生物学 Q3 CELL BIOLOGY Experimental cell research Pub Date : 2024-09-19 DOI:10.1016/j.yexcr.2024.114262
You Fu , Chen-Jun Guo , Zhi-Jie Liu , Ji-Long Liu
{"title":"低温电子断层扫描揭示的 CTPS 纤维网络结构。","authors":"You Fu ,&nbsp;Chen-Jun Guo ,&nbsp;Zhi-Jie Liu ,&nbsp;Ji-Long Liu","doi":"10.1016/j.yexcr.2024.114262","DOIUrl":null,"url":null,"abstract":"<div><div>The cytoophidium is a novel type of membraneless organelle, first observed in the ovaries of <em>Drosophila</em> using fluorescence microscopy. <em>In vitro</em>, purified <em>Drosophila melanogaster</em> CTPS (dmCTPS) can form metabolic filaments under the presence of either substrates or products, and their structures that have been analyzed using cryo-electron microscopy (cryo-EM). These dmCTPS filaments are considered the fundamental units of cytoophidia. However, due to the resolution gap between light and electron microscopy, the precise assembly pattern of cytoophidia remains unclear. In this study, we find that dmCTPS filaments can spontaneously assemble <em>in vitro</em>, forming network structures that reach micron-scale dimensions. Using cryo-electron tomography (cryo-ET), we reconstruct the network structures formed by dmCTPS filaments under substrate or product binding conditions and elucidate their assembly process. The dmCTPS filaments initially form structural bundles, which then further assemble into larger networks. By identifying, tracking, and statistically analyzing the filaments, we observed distinct characteristics of the structural bundles formed under different conditions. This study provides the first systematic analysis of dmCTPS filament networks, offering new insights into the relationship between cytoophidia and metabolic filaments.</div></div>","PeriodicalId":12227,"journal":{"name":"Experimental cell research","volume":"442 2","pages":"Article 114262"},"PeriodicalIF":3.3000,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0014482724003537/pdfft?md5=24f45cf814818b4c23776844d32d213c&pid=1-s2.0-S0014482724003537-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Architecture of CTPS filament networks revealed by cryo-electron tomography\",\"authors\":\"You Fu ,&nbsp;Chen-Jun Guo ,&nbsp;Zhi-Jie Liu ,&nbsp;Ji-Long Liu\",\"doi\":\"10.1016/j.yexcr.2024.114262\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The cytoophidium is a novel type of membraneless organelle, first observed in the ovaries of <em>Drosophila</em> using fluorescence microscopy. <em>In vitro</em>, purified <em>Drosophila melanogaster</em> CTPS (dmCTPS) can form metabolic filaments under the presence of either substrates or products, and their structures that have been analyzed using cryo-electron microscopy (cryo-EM). These dmCTPS filaments are considered the fundamental units of cytoophidia. However, due to the resolution gap between light and electron microscopy, the precise assembly pattern of cytoophidia remains unclear. In this study, we find that dmCTPS filaments can spontaneously assemble <em>in vitro</em>, forming network structures that reach micron-scale dimensions. Using cryo-electron tomography (cryo-ET), we reconstruct the network structures formed by dmCTPS filaments under substrate or product binding conditions and elucidate their assembly process. The dmCTPS filaments initially form structural bundles, which then further assemble into larger networks. By identifying, tracking, and statistically analyzing the filaments, we observed distinct characteristics of the structural bundles formed under different conditions. This study provides the first systematic analysis of dmCTPS filament networks, offering new insights into the relationship between cytoophidia and metabolic filaments.</div></div>\",\"PeriodicalId\":12227,\"journal\":{\"name\":\"Experimental cell research\",\"volume\":\"442 2\",\"pages\":\"Article 114262\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2024-09-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0014482724003537/pdfft?md5=24f45cf814818b4c23776844d32d213c&pid=1-s2.0-S0014482724003537-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Experimental cell research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0014482724003537\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental cell research","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0014482724003537","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

嗜细胞器是一种新型的无膜细胞器,最早是在果蝇的卵巢中利用荧光显微镜观察到的。在体外,纯化的黑腹果蝇 CTPS(dmCTPS)可以在底物或产物存在的情况下形成代谢丝,其结构已通过冷冻电镜(cryo-EM)进行了分析。这些 dmCTPS 丝被认为是细胞噬菌体的基本单位。然而,由于光学显微镜和电子显微镜之间的分辨率差距,细胞噬纤维的精确组装模式仍不清楚。在这项研究中,我们发现 dmCTPS 细丝可在体外自发组装,形成达到微米级尺寸的网络结构。我们利用低温电子断层扫描(cryo-ET)重建了 dmCTPS 细丝在底物或产物结合条件下形成的网络结构,并阐明了它们的组装过程。dmCTPS 细丝最初形成结构束,然后进一步组装成更大的网络。通过识别、跟踪和统计分析这些丝状物,我们观察到在不同条件下形成的结构束具有不同的特征。这项研究首次对 dmCTPS 细丝网络进行了系统分析,为了解细胞膜和代谢细丝之间的关系提供了新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Architecture of CTPS filament networks revealed by cryo-electron tomography
The cytoophidium is a novel type of membraneless organelle, first observed in the ovaries of Drosophila using fluorescence microscopy. In vitro, purified Drosophila melanogaster CTPS (dmCTPS) can form metabolic filaments under the presence of either substrates or products, and their structures that have been analyzed using cryo-electron microscopy (cryo-EM). These dmCTPS filaments are considered the fundamental units of cytoophidia. However, due to the resolution gap between light and electron microscopy, the precise assembly pattern of cytoophidia remains unclear. In this study, we find that dmCTPS filaments can spontaneously assemble in vitro, forming network structures that reach micron-scale dimensions. Using cryo-electron tomography (cryo-ET), we reconstruct the network structures formed by dmCTPS filaments under substrate or product binding conditions and elucidate their assembly process. The dmCTPS filaments initially form structural bundles, which then further assemble into larger networks. By identifying, tracking, and statistically analyzing the filaments, we observed distinct characteristics of the structural bundles formed under different conditions. This study provides the first systematic analysis of dmCTPS filament networks, offering new insights into the relationship between cytoophidia and metabolic filaments.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Experimental cell research
Experimental cell research 医学-细胞生物学
CiteScore
7.20
自引率
0.00%
发文量
295
审稿时长
30 days
期刊介绍: Our scope includes but is not limited to areas such as: Chromosome biology; Chromatin and epigenetics; DNA repair; Gene regulation; Nuclear import-export; RNA processing; Non-coding RNAs; Organelle biology; The cytoskeleton; Intracellular trafficking; Cell-cell and cell-matrix interactions; Cell motility and migration; Cell proliferation; Cellular differentiation; Signal transduction; Programmed cell death.
期刊最新文献
Editorial Board The cystogenic effects of ouabain in autosomal dominant polycystic kidney disease require cell caveolae. Fbxo11 maintains mitochondrial function and prevents podocyte injury in adriamycin-induced nephropathy by mediating the ubiquitin degradation of Fosl2. MOTS-c relieves hepatocellular carcinoma resistance to TRAIL-induced apoptosis under hypoxic conditions by activating MEF2A DDX18 influences chemotherapy sensitivity in colorectal cancer by regulating genomic stability
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1