WTAP/CCND1轴通过MAPK信号通路加速食管鳞状细胞癌的进展。

IF 2 4区 医学 Q3 ONCOLOGY Neoplasma Pub Date : 2024-08-01 DOI:10.4149/neo_2024_231219N653
Hong Zhang, Xiaojing Zhang, Yan Zhang, Jianhua Wu, Jiali Li, Baoen Shan
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引用次数: 0

摘要

近年来,N6-甲基腺苷(m6A)甲基化作为一种新的调控机制,被报道参与了多种生物过程。Wilms tumor 1-associated protein(WTAP)作为 m6A 甲基化的关键成员,已被证实参与了肿瘤的发生。在此,我们研究了 WTAP 的表达及其参与食管鳞状细胞癌(ESCC)发生的潜在机制。我们检测了 WTAP 的表达及其与临床病理特征的相关性,并通过 MTS 试验、集落形成试验、划痕伤口愈合试验、Transwell 试验和皮下异种移植试验确定了 WTAP 对 ESCC 细胞的作用。我们利用 mRNA 测序技术筛选了 WTAP 的候选下游靶点,并通过一系列挽救实验研究了 CCND1 促进 ESCC 的内在机制。WTAP在ESCC恶性肿瘤中的表达升高表明预后较差。WTAP促进了ESCC细胞的增殖和转移,而CCND1被确定为WTAP的潜在下游效应器。此外,WTAP通过MAPK通路依赖模式调节ESCC的进展。我们的研究表明,WTAP通过MAPK信号通路加速CCND1的表达,从而促进了ESCC的增殖和转移,这表明WTAP可能是ESCC的一种候选预后生物标志物,也将是ESCC癌症治疗的一种有前途的策略。
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WTAP/CCND1 axis accelerates esophageal squamous cell carcinoma progression by MAPK signaling pathway.

N6-methyladenosine (m6A) methylation, as a new regulatory mechanism, has been reported to be involved in diverse biological processes in recent years. Wilms tumor 1-associated protein (WTAP), as the key member of m6A methylation, has been proven to participate in tumorigenesis. Here, we studied the expression of WTAP and its potential mechanism involved in the development of esophageal squamous cell carcinoma (ESCC). We detected the expression of WTAP and its correlation with clinicopathological features, and we determined the function of WTAP on ESCC cells by MTS assay, colony formation, scratch wound healing assay, Transwell assay, and subcutaneous xenograft assay. We used mRNA sequencing technology to screen candidate downstream targets for WTAP and investigated the underlying mechanism of CCND1 in ESCC promotion through a series of rescue assays. An elevated expression of WTAP in ESCC malignancy indicated a worse prognosis. WTAP promoted the proliferation and metastasis of ESCC cells, and CCND1 was identified as the potential downstream effecter of WTAP. Moreover, WTAP modulated ESCC progression through a MAPK pathway-dependent pattern. Our research suggested that WTAP promoted both proliferation and metastasis of ESCC by accelerating the expression of CCND1 via the MAPK signaling pathway, indicating that WTAP may be a candidate prognostic biomarker for ESCC and also will be a promising strategy for ESCC cancer therapy.

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来源期刊
Neoplasma
Neoplasma 医学-肿瘤学
CiteScore
5.40
自引率
0.00%
发文量
238
审稿时长
3 months
期刊介绍: The journal Neoplasma publishes articles on experimental and clinical oncology and cancer epidemiology.
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