利用声学流体系统提高全血中血小板的分离性能。

IF 4.5 2区 医学 Q1 ONCOLOGY Cancer Science Pub Date : 2024-09-22 DOI:10.1111/cas.16337
Kazuko Sakai, Shuta Ohara, Junko Tanaka, Kenichi Suda, Takamichi Muramatsu, Chihiro Uematsu, Yasuhiro Tsutani, Tetsuya Mitsudomi, Kazuto Nishio
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引用次数: 0

摘要

本研究调查了声学分离法与传统离心法在非小细胞肺癌(NSCLC)患者血小板分析中的有效性。共有 10 名非小细胞肺癌患者和 10 名健康志愿者提供了外周血样本,采用声学分离法或离心法对样本进行处理,以分离血小板。研究包括对血小板、外周血单核细胞和肿瘤组织样本进行全转录组分析,采用层次聚类和基因本体分析来探讨基因表达差异。事实证明,就血小板产量、回收率和 RNA 产量而言,声学分离比离心分离更有效。与健康志愿者相比,NSCLC 患者血小板的基因表达谱显示出不同的模式,表明受肿瘤影响的改变。基因本体分析显示,与血小板活化和肿瘤微环境相关的通路得到了丰富。这一发现表明了声学分离技术在血小板分离中的潜力,以及它在了解 NSCLC 患者血小板独特基因表达谱方面的相关性。这项研究的结果表明,通过声学技术分离的癌症患者血小板表现出肿瘤特异性改变,为临床实践中血小板分析系统的癌症诊断提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Improved platelet separation performance from whole blood using an acoustic fluidics system

This study investigated the effectiveness of acoustic separation for platelet analysis in patients with non–small-cell lung cancer (NSCLC), comparing it with traditional centrifugation methods. In total, 10 patients with NSCLC and 10 healthy volunteers provided peripheral blood samples, which were processed using either acoustic separation or centrifugation to isolate platelets. The study included whole transcriptome analysis of platelets, peripheral blood mononuclear cells, and tumor tissue samples, employing hierarchical clustering and Gene Ontology analysis to explore gene expression differences. Acoustic separation proved more efficient than centrifugation in terms of platelet yield, recovery rate, and RNA yield. Gene expression profiles of platelets from patients with NSCLC showed distinct patterns compared with healthy volunteers, indicating tumor-influenced alterations. Gene Ontology analysis revealed enrichment in pathways associated with platelet activation and the tumor microenvironment. This finding indicates the potential of acoustic isolation in platelet separation and its relevance in understanding the unique gene expression profile of platelets in patients with NSCLC. The findings of this study suggested that platelets from cancer patients separated by acoustic techniques exhibited tumor-specific alterations and provided new insights into the diagnosis of cancer in platelet analysis systems in clinical practice.

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来源期刊
Cancer Science
Cancer Science 医学-肿瘤学
自引率
3.50%
发文量
406
审稿时长
2 months
期刊介绍: Cancer Science (formerly Japanese Journal of Cancer Research) is a monthly publication of the Japanese Cancer Association. First published in 1907, the Journal continues to publish original articles, editorials, and letters to the editor, describing original research in the fields of basic, translational and clinical cancer research. The Journal also accepts reports and case reports. Cancer Science aims to present highly significant and timely findings that have a significant clinical impact on oncologists or that may alter the disease concept of a tumor. The Journal will not publish case reports that describe a rare tumor or condition without new findings to be added to previous reports; combination of different tumors without new suggestive findings for oncological research; remarkable effect of already known treatments without suggestive data to explain the exceptional result. Review articles may also be published.
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Issue Information In this issue Issue Information In this issue Real-world genome profiling in Japanese patients with pancreatic ductal adenocarcinoma focusing on HRD implications
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