Jie Zheng , Jingru Ji , Wei Chen , Danwei Wang , Chang Liu , Yan Zhang , Han Shen , Xiaoli Cao , Chao Wu
{"title":"在一家三级医院中，被误认为鲍曼不动杆菌的非鲍曼不动杆菌存在编码碳青霉烯水解氧青霉烯酶的基因，且对碳青霉烯类无耐药性。","authors":"Jie Zheng , Jingru Ji , Wei Chen , Danwei Wang , Chang Liu , Yan Zhang , Han Shen , Xiaoli Cao , Chao Wu","doi":"10.1016/j.meegid.2024.105669","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>This study aims to analyze the genomic and clinical characteristics of Non-baumannii <em>Acinetobacter</em> strains misidentified as <em>A. baumannii</em>, causing bloodstream infections (BSIs) in our hospital.</div></div><div><h3>Materials and methods</h3><div>Whole genome sequencing was performed and average nucleotide identity (ANI) was analyzed. Susceptibility testing was conducted using micro-broth methods. The distribution of antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs) was examined using online software tools. The prevalence of virulence factors (VFs) was investigated through nucleotide coding sequence comparisons. Genetic structures of <em>bla</em>OXA genes were analyzed by Gcluster software. Clinical information was collected from electronic medical records for patient characterization.</div></div><div><h3>Results</h3><div>ANI analysis identified five strains as <em>Acinetobacter pittii</em>, with the remaining four identified as <em>A. geminorum</em>, <em>A. nosocomialis</em>, <em>A. soli</em> and <em>A. bereziniae</em>. The GC content of all isolates was less than 38.9 % except for <em>A. soli</em> 16,294. All Non-baumannii <em>Acinetobacter</em> strains were relatively susceptible to antibiotics, except for one <em>A. pittii</em> isolate. Nine <em>bla</em>OXA variants were identified in seven isolates, with two isolates co-carrying 2 different types of <em>bla</em>OXA. Twenty-four insertion sequences (ISs) were identified, with ISAba and IS17 being the primary ISs. Five <em>A. pittii</em> isolates shared the same genetic structures around <em>bla</em>OXA. Genes related to adherence, immune modulation, and nutritional/metabolic factors were the most frequent. Few VFs were detected in <em>A. soli</em> 16,294 and <em>A.bereziniae</em> 14,325.</div></div><div><h3>Conclusions</h3><div>The presence of carbapenem hydrolyzing oxacillinase encoding genes did not confer carbapenem resistance, possibly due to the lack of ISs in the <em>bla</em>OXA flanking sequences. Different <em>bla</em>OXA variants within distinct strains shared the same genetic structures, suggesting potential for multidrug resistance development, which warrants our attention.</div></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":"125 ","pages":"Article 105669"},"PeriodicalIF":2.6000,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567134824001205/pdfft?md5=3457782c0565ca872d7f4c3ed085bf49&pid=1-s2.0-S1567134824001205-main.pdf","citationCount":"0","resultStr":"{\"title\":\"The presence of genes encoding carbapenem-hydrolyzing oxacillinase and lack of carbapenem resistance in non-baumannii Acinetobacter misidentified as Acinetobacter baumannii causing bloodstream infections in a tertiary hospital over a 3-year period\",\"authors\":\"Jie Zheng , Jingru Ji , Wei Chen , Danwei Wang , Chang Liu , Yan Zhang , Han Shen , Xiaoli Cao , Chao Wu\",\"doi\":\"10.1016/j.meegid.2024.105669\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><div>This study aims to analyze the genomic and clinical characteristics of Non-baumannii <em>Acinetobacter</em> strains misidentified as <em>A. baumannii</em>, causing bloodstream infections (BSIs) in our hospital.</div></div><div><h3>Materials and methods</h3><div>Whole genome sequencing was performed and average nucleotide identity (ANI) was analyzed. Susceptibility testing was conducted using micro-broth methods. The distribution of antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs) was examined using online software tools. The prevalence of virulence factors (VFs) was investigated through nucleotide coding sequence comparisons. Genetic structures of <em>bla</em>OXA genes were analyzed by Gcluster software. Clinical information was collected from electronic medical records for patient characterization.</div></div><div><h3>Results</h3><div>ANI analysis identified five strains as <em>Acinetobacter pittii</em>, with the remaining four identified as <em>A. geminorum</em>, <em>A. nosocomialis</em>, <em>A. soli</em> and <em>A. bereziniae</em>. The GC content of all isolates was less than 38.9 % except for <em>A. soli</em> 16,294. All Non-baumannii <em>Acinetobacter</em> strains were relatively susceptible to antibiotics, except for one <em>A. pittii</em> isolate. Nine <em>bla</em>OXA variants were identified in seven isolates, with two isolates co-carrying 2 different types of <em>bla</em>OXA. Twenty-four insertion sequences (ISs) were identified, with ISAba and IS17 being the primary ISs. Five <em>A. pittii</em> isolates shared the same genetic structures around <em>bla</em>OXA. Genes related to adherence, immune modulation, and nutritional/metabolic factors were the most frequent. Few VFs were detected in <em>A. soli</em> 16,294 and <em>A.bereziniae</em> 14,325.</div></div><div><h3>Conclusions</h3><div>The presence of carbapenem hydrolyzing oxacillinase encoding genes did not confer carbapenem resistance, possibly due to the lack of ISs in the <em>bla</em>OXA flanking sequences. Different <em>bla</em>OXA variants within distinct strains shared the same genetic structures, suggesting potential for multidrug resistance development, which warrants our attention.</div></div>\",\"PeriodicalId\":54986,\"journal\":{\"name\":\"Infection Genetics and Evolution\",\"volume\":\"125 \",\"pages\":\"Article 105669\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-09-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1567134824001205/pdfft?md5=3457782c0565ca872d7f4c3ed085bf49&pid=1-s2.0-S1567134824001205-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Infection Genetics and Evolution\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1567134824001205\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Infection Genetics and Evolution","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1567134824001205","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
The presence of genes encoding carbapenem-hydrolyzing oxacillinase and lack of carbapenem resistance in non-baumannii Acinetobacter misidentified as Acinetobacter baumannii causing bloodstream infections in a tertiary hospital over a 3-year period
Objective
This study aims to analyze the genomic and clinical characteristics of Non-baumannii Acinetobacter strains misidentified as A. baumannii, causing bloodstream infections (BSIs) in our hospital.
Materials and methods
Whole genome sequencing was performed and average nucleotide identity (ANI) was analyzed. Susceptibility testing was conducted using micro-broth methods. The distribution of antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs) was examined using online software tools. The prevalence of virulence factors (VFs) was investigated through nucleotide coding sequence comparisons. Genetic structures of blaOXA genes were analyzed by Gcluster software. Clinical information was collected from electronic medical records for patient characterization.
Results
ANI analysis identified five strains as Acinetobacter pittii, with the remaining four identified as A. geminorum, A. nosocomialis, A. soli and A. bereziniae. The GC content of all isolates was less than 38.9 % except for A. soli 16,294. All Non-baumannii Acinetobacter strains were relatively susceptible to antibiotics, except for one A. pittii isolate. Nine blaOXA variants were identified in seven isolates, with two isolates co-carrying 2 different types of blaOXA. Twenty-four insertion sequences (ISs) were identified, with ISAba and IS17 being the primary ISs. Five A. pittii isolates shared the same genetic structures around blaOXA. Genes related to adherence, immune modulation, and nutritional/metabolic factors were the most frequent. Few VFs were detected in A. soli 16,294 and A.bereziniae 14,325.
Conclusions
The presence of carbapenem hydrolyzing oxacillinase encoding genes did not confer carbapenem resistance, possibly due to the lack of ISs in the blaOXA flanking sequences. Different blaOXA variants within distinct strains shared the same genetic structures, suggesting potential for multidrug resistance development, which warrants our attention.
期刊介绍：
(aka Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases -- MEEGID)
Infectious diseases constitute one of the main challenges to medical science in the coming century. The impressive development of molecular megatechnologies and of bioinformatics have greatly increased our knowledge of the evolution, transmission and pathogenicity of infectious diseases. Research has shown that host susceptibility to many infectious diseases has a genetic basis. Furthermore, much is now known on the molecular epidemiology, evolution and virulence of pathogenic agents, as well as their resistance to drugs, vaccines, and antibiotics. Equally, research on the genetics of disease vectors has greatly improved our understanding of their systematics, has increased our capacity to identify target populations for control or intervention, and has provided detailed information on the mechanisms of insecticide resistance.
However, the genetics and evolutionary biology of hosts, pathogens and vectors have tended to develop as three separate fields of research. This artificial compartmentalisation is of concern due to our growing appreciation of the strong co-evolutionary interactions among hosts, pathogens and vectors.
Infection, Genetics and Evolution and its companion congress [MEEGID](http://www.meegidconference.com/) (for Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases) are the main forum acting for the cross-fertilization between evolutionary science and biomedical research on infectious diseases.
Infection, Genetics and Evolution is the only journal that welcomes articles dealing with the genetics and evolutionary biology of hosts, pathogens and vectors, and coevolution processes among them in relation to infection and disease manifestation. All infectious models enter the scope of the journal, including pathogens of humans, animals and plants, either parasites, fungi, bacteria, viruses or prions. The journal welcomes articles dealing with genetics, population genetics, genomics, postgenomics, gene expression, evolutionary biology, population dynamics, mathematical modeling and bioinformatics. We also provide many author benefits, such as free PDFs, a liberal copyright policy, special discounts on Elsevier publications and much more. Please click here for more information on our author services .
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