香芹酚水凝胶对牙周炎大鼠牙槽骨的保护作用

Lulu Zhou, Nian Teng, Tiantian Gao, Hongbin Wang, Xiang Gao
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引用次数: 0

摘要

研究目的本研究旨在探讨香芹酚水凝胶对牙周炎大鼠牙槽骨的保护作用及其机制:以聚氧乙烯醚和羟丙基甲基纤维素为基质制备了以香芹酚为载体的热敏水凝胶。将 SD 大鼠随机分为空白组、牙周炎组、空白水凝胶组和低、中、高剂量水凝胶组。观察牙周炎症状和牙槽骨 CT 结构。观察肝、脾、肾和牙周组织的变化。检测血清中骨代谢的相关指标。通过 Western 印迹法测定了骨保护素(OPG)和核转录因子-κB(NF-κB)通路蛋白的表达。通过定量反转录聚合酶链反应(qRT-PCR)评估炎症因子的水平:结果:香芹酚水凝胶具有良好的缓释性、生物相容性和细胞粘附性。结果:香芹酚水凝胶具有良好的缓释性、生物相容性和细胞粘附性,香芹酚水凝胶组大鼠的牙周炎明显缓解,牙龈组织中 OPG 蛋白的表达明显增加(PPConclusions:香芹酚水凝胶能调节 OPG 和 NF-κB 通路,减少牙槽骨吸收,改善牙周炎症。
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Protective effect of carvacrol hydrogel on the alveolar bone in rats with periodontitis.

Objectives: This study aimed to investigate the protective effect and mechanism of carvacrol hydrogel on the alveolar bone in rats with periodontitis.

Methods: A thermosensitive hydrogel supported by carvacrol was prepared using poloxamer and hydroxypropyl methyl cellulose as matrix. SD rats were randomly divided into blank group, periodontitis group, blank hydrogel group, and low-, medium-, and high-dose hydrogel groups. The periodontitis symptoms and the CT structure of the alveolar bone were observed. The changes in liver, spleen, kidney, and periodontal tissues were observed. The related indexes of bone metabolism in serum were detected. The expression of osteoprotegerin (OPG) and nuclear transcription factor-κB (NF-κB) pathway proteins was determined by Western blot. The levels of inflammatory factors were assessed by quantitative reverse transcription polymerase chain reaction (qRT-PCR).

Results: Carvacrol hydrogel had good slow release, biocompatibility, and cell adhesion. The periodontitis of rats in the carvacrol hydrogel group was significantly alleviated, the expression of OPG protein in gingival tissue was significantly increased (P<0.01), and the levels of receptor activator of NF-κB ligand (RANKL), receptor activator of NF-κB (RANK), NF-κB protein, and inflammatory factors were significantly decreased (P<0.01).

Conclusions: Carvacrol hydrogel can regulate the OPG and NF-κB pathways, reduce alveolar bone absorption, and improve periodontal inflammation.

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