{"title":"TET 酶合成模型选择性催化羟甲基胞嘧啶转化为甲酰基胞嘧啶","authors":"Dipanwita Palit, Debasish Manna","doi":"10.1039/d4qi01965b","DOIUrl":null,"url":null,"abstract":"The TET enzymes, known as the ten-eleven translocation enzymes, have become central figures in epigenetic regulation due to their remarkable ability to oxidize 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC), 5-formylcytosine (5-fC), 5-carboxy cytosine (5-caC) thus influencing gene expression and DNA methylation patterns. Understanding the intricate mechanisms underlying TET enzyme function is crucial for unraveling epigenetic regulatory pathways and their implications in various biological processes, including development, differentiation, and disease progression. Recently, we have shown that FeIIITAML complex acts as a synthetic model of TET enzyme by selectively oxidizing 5-hmC to 5-fC. Herein we report another synthetic model, FeIIIbTAML, for selective and catalytic oxidation of 5-hmC. The current synthetic model overcomes several limitations of the previous TET model reported by us. In addition to oxidizing simple nucleobase, we have shown that the FeIIIbTAML, in the presence of H2O2, can selectively oxidize nucleoside and small DNA fragments containing 5-hmC in a catalytic manner.","PeriodicalId":79,"journal":{"name":"Inorganic Chemistry Frontiers","volume":null,"pages":null},"PeriodicalIF":6.1000,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Selective and Catalytic Conversion of Hydroxymethyl Cytosine to Formyl Cytosine by a Synthetic Model of TET Enzymes\",\"authors\":\"Dipanwita Palit, Debasish Manna\",\"doi\":\"10.1039/d4qi01965b\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The TET enzymes, known as the ten-eleven translocation enzymes, have become central figures in epigenetic regulation due to their remarkable ability to oxidize 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC), 5-formylcytosine (5-fC), 5-carboxy cytosine (5-caC) thus influencing gene expression and DNA methylation patterns. Understanding the intricate mechanisms underlying TET enzyme function is crucial for unraveling epigenetic regulatory pathways and their implications in various biological processes, including development, differentiation, and disease progression. Recently, we have shown that FeIIITAML complex acts as a synthetic model of TET enzyme by selectively oxidizing 5-hmC to 5-fC. Herein we report another synthetic model, FeIIIbTAML, for selective and catalytic oxidation of 5-hmC. The current synthetic model overcomes several limitations of the previous TET model reported by us. In addition to oxidizing simple nucleobase, we have shown that the FeIIIbTAML, in the presence of H2O2, can selectively oxidize nucleoside and small DNA fragments containing 5-hmC in a catalytic manner.\",\"PeriodicalId\":79,\"journal\":{\"name\":\"Inorganic Chemistry Frontiers\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":6.1000,\"publicationDate\":\"2024-09-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Inorganic Chemistry Frontiers\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1039/d4qi01965b\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, INORGANIC & NUCLEAR\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Inorganic Chemistry Frontiers","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1039/d4qi01965b","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, INORGANIC & NUCLEAR","Score":null,"Total":0}
Selective and Catalytic Conversion of Hydroxymethyl Cytosine to Formyl Cytosine by a Synthetic Model of TET Enzymes
The TET enzymes, known as the ten-eleven translocation enzymes, have become central figures in epigenetic regulation due to their remarkable ability to oxidize 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC), 5-formylcytosine (5-fC), 5-carboxy cytosine (5-caC) thus influencing gene expression and DNA methylation patterns. Understanding the intricate mechanisms underlying TET enzyme function is crucial for unraveling epigenetic regulatory pathways and their implications in various biological processes, including development, differentiation, and disease progression. Recently, we have shown that FeIIITAML complex acts as a synthetic model of TET enzyme by selectively oxidizing 5-hmC to 5-fC. Herein we report another synthetic model, FeIIIbTAML, for selective and catalytic oxidation of 5-hmC. The current synthetic model overcomes several limitations of the previous TET model reported by us. In addition to oxidizing simple nucleobase, we have shown that the FeIIIbTAML, in the presence of H2O2, can selectively oxidize nucleoside and small DNA fragments containing 5-hmC in a catalytic manner.