沙眼衣原体血清学候选抗体参考试剂

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS Journal of immunological methods Pub Date : 2024-09-24 DOI:10.1016/j.jim.2024.113761

Filomeno Coelho da Silva , Gathoni Kamuyu , Birgitta Michels , Jessica Edney , Laura Hassall , Paul Stickings , Sunil Maharjan , Tim Waterboer , Simon Beddows

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引用次数: 0

摘要

沙眼衣原体（Ct）血清学是监测感染和疾病负担的重要工具，但目前还没有正式的参考试剂来统一结果报告。我们的目标是开发一组可与主要外膜蛋白（MOMP）和毒力因子（pgp3）抗原发生反应的候选参考试剂。我们对女性（20-40 岁）的血浆包进行了针对 MOMP 和 pgp3 抗原的筛选，并将筛选出的阳性和阴性样本集中起来，以建立一个候选抗体参考试剂组，在两个实验室进行测试。此外，还对抗原特异性和内部质量保证进行了评估。已选择合适的候选材料来生产 Ct 参考试剂。

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Candidate antibody reference reagents for Chlamydia trachomatis serology

Chlamydia trachomatis (Ct) serology is an important tool for monitoring infection and disease burden but there are currently no formal reference reagents to harmonize results reporting. Our objective was to develop a panel of candidate reference reagents with reactivity against the major outer membrane protein (MOMP) and virulence factor (pgp3) antigens. Plasma packs from females (20–40 years old) were screened against MOMP and pgp3 antigens and selected positive and negative samples pooled to create a panel of candidate antibody reference reagents that were tested in two laboratories. Antigen specificity and internal quality assurance were also evaluated. Suitable candidate materials have been selected to produce Ct reference reagents.

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来源期刊

Journal of immunological methods 医学-免疫学

CiteScore

4.10

自引率

0.00%

发文量

120

审稿时长

3 months

期刊介绍： The Journal of Immunological Methods is devoted to covering techniques for: (1) Quantitating and detecting antibodies and/or antigens. (2) Purifying immunoglobulins, lymphokines and other molecules of the immune system. (3) Isolating antigens and other substances important in immunological processes. (4) Labelling antigens and antibodies. (5) Localizing antigens and/or antibodies in tissues and cells. (6) Detecting, and fractionating immunocompetent cells. (7) Assaying for cellular immunity. (8) Documenting cell-cell interactions. (9) Initiating immunity and unresponsiveness. (10) Transplanting tissues. (11) Studying items closely related to immunity such as complement, reticuloendothelial system and others. (12) Molecular techniques for studying immune cells and their receptors. (13) Imaging of the immune system. (14) Methods for production or their fragments in eukaryotic and prokaryotic cells. In addition the journal will publish articles on novel methods for analysing the organization, structure and expression of genes for immunologically important molecules such as immunoglobulins, T cell receptors and accessory molecules involved in antigen recognition, processing and presentation. Submitted full length manuscripts should describe new methods of broad applicability to immunology and not simply the application of an established method to a particular substance - although papers describing such applications may be considered for publication as a short Technical Note. Review articles will also be published by the Journal of Immunological Methods. In general these manuscripts are by solicitation however anyone interested in submitting a review can contact the Reviews Editor and provide an outline of the proposed review.