{"title":"从生物启发出发,将肽、咪唑甲醛和铜共同组装成具有类似长效酶活性的纳米酶,用于肾上腺素的比色检测","authors":"","doi":"10.1016/j.colsurfa.2024.135445","DOIUrl":null,"url":null,"abstract":"<div><div>Epinephrine plays a vital role in the central nervous system, but its abnormal levels will result in various diseases. Therefore, facile, rapid and accurate detection of epinephrine is essential for clinical application. Due to its phenolic structure, epinephrine can be detected through colorimetric assays as it is oxidized by laccase to form a colored quinone compound. In this work, a laccase-like nanozyme, diphenylalanine peptide/4-imidazolecarboxaldehyde-Cu (FF/ICA-Cu), was designed to mimic the active center of natural laccase. The as-synthesized FF/ICA-Cu nanozyme showed high laccase-like activity because of its structural similarity to the natural laccase active center. Moreover, it exhibited excellent stability and recyclability, even under harsh conditions. Based on its laccase-like activity, a sensitive and selective sensing strategy for epinephrine was developed. This approach offers a promising avenue for accurately detecting epinephrine and designing catalysts with potential for future industrial applications.</div></div>","PeriodicalId":278,"journal":{"name":"Colloids and Surfaces A: Physicochemical and Engineering Aspects","volume":null,"pages":null},"PeriodicalIF":4.9000,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bioinspired coassembly of peptide, imidazolecarboxaldehyde, and copper for nanozyme with laccase-like activity for colorimetric detection of epinephrine\",\"authors\":\"\",\"doi\":\"10.1016/j.colsurfa.2024.135445\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Epinephrine plays a vital role in the central nervous system, but its abnormal levels will result in various diseases. Therefore, facile, rapid and accurate detection of epinephrine is essential for clinical application. Due to its phenolic structure, epinephrine can be detected through colorimetric assays as it is oxidized by laccase to form a colored quinone compound. In this work, a laccase-like nanozyme, diphenylalanine peptide/4-imidazolecarboxaldehyde-Cu (FF/ICA-Cu), was designed to mimic the active center of natural laccase. The as-synthesized FF/ICA-Cu nanozyme showed high laccase-like activity because of its structural similarity to the natural laccase active center. Moreover, it exhibited excellent stability and recyclability, even under harsh conditions. Based on its laccase-like activity, a sensitive and selective sensing strategy for epinephrine was developed. This approach offers a promising avenue for accurately detecting epinephrine and designing catalysts with potential for future industrial applications.</div></div>\",\"PeriodicalId\":278,\"journal\":{\"name\":\"Colloids and Surfaces A: Physicochemical and Engineering Aspects\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.9000,\"publicationDate\":\"2024-09-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Colloids and Surfaces A: Physicochemical and Engineering Aspects\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0927775724023094\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Colloids and Surfaces A: Physicochemical and Engineering Aspects","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0927775724023094","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
Bioinspired coassembly of peptide, imidazolecarboxaldehyde, and copper for nanozyme with laccase-like activity for colorimetric detection of epinephrine
Epinephrine plays a vital role in the central nervous system, but its abnormal levels will result in various diseases. Therefore, facile, rapid and accurate detection of epinephrine is essential for clinical application. Due to its phenolic structure, epinephrine can be detected through colorimetric assays as it is oxidized by laccase to form a colored quinone compound. In this work, a laccase-like nanozyme, diphenylalanine peptide/4-imidazolecarboxaldehyde-Cu (FF/ICA-Cu), was designed to mimic the active center of natural laccase. The as-synthesized FF/ICA-Cu nanozyme showed high laccase-like activity because of its structural similarity to the natural laccase active center. Moreover, it exhibited excellent stability and recyclability, even under harsh conditions. Based on its laccase-like activity, a sensitive and selective sensing strategy for epinephrine was developed. This approach offers a promising avenue for accurately detecting epinephrine and designing catalysts with potential for future industrial applications.
期刊介绍:
Colloids and Surfaces A: Physicochemical and Engineering Aspects is an international journal devoted to the science underlying applications of colloids and interfacial phenomena.
The journal aims at publishing high quality research papers featuring new materials or new insights into the role of colloid and interface science in (for example) food, energy, minerals processing, pharmaceuticals or the environment.