一种多组学方法揭示了莲藕多糖铁通过激活PPARγ促进类固醇激素合成来改善缺铁引起的睾丸损伤。

Pei-Yu Dong, Shuai Yuan, Yu-Mei Chen Yan, Yu Chen, Yue Bai, Yang Dong, Yin-Yin Li, Wei Shen, Xi-Feng Zhang
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引用次数: 0

摘要

缺铁是严重影响男性生殖健康的常见营养问题。莲藕多糖铁(LRPF)是一种新型营养补充剂,可改善铁缺乏造成的损害,但其机制尚不清楚。在这项研究中,我们通过整合转录组学、微生物组学和血清代谢组学,全面测定了LRPF对缺铁小鼠生殖的益处。微生物组学结果表明,LRPF能恢复缺铁引起的肠道微生物群变化。代谢组学结果显示,LRPF能稳定缺铁小鼠的类固醇激素和脂肪酸代谢,降低菊酸乙酯(EC)的含量,改善生殖功能障碍。转录组分析表明,LRPF能调节缺铁小鼠体内类固醇激素的合成和过氧化物酶体增殖激活受体(PPAR)信号通路。体外实验表明,LRPF 可通过激活 PPARγ 促进莱地格细胞中类固醇激素的合成。总之,这项研究强调了 LRPF 在改善睾丸发育方面的优势。
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A multi-omics approach reveals that lotus root polysaccharide iron ameliorates iron deficiency-induced testicular damage by activating PPARγ to promote steroid hormone synthesis.

Iron deficiency is a common nutritional issue that seriously affects male reproductive health. Lotus root polysaccharide iron (LRPF), a novel nutritional supplement, may ameliorate the damage caused by iron deficiency, however, the mechanism is unclear. In this study, we comprehensively determined the benefits of LRPF on reproduction in iron-deficient mice by integrating transcriptomics, microbiomics and serum metabolomics. Microbiomics showed that LRPF could restore changes to the intestinal microbiota caused by iron deficiency. Metabolomics results showed that LRPF stabilised steroid hormone and fatty acid metabolism in iron-deficient mice, reduced the content of ethyl chrysanthemumate (EC) and ameliorated the reproductive impairment. The transcriptomic analysis showed that LRPF regulated steroid hormone synthesis and the peroxisome proliferator-activated receptor (PPAR) signalling pathway in iron-deficient mice. In vitro experiments showed that LRPF could promote steroid hormone synthesis in Leydig cells by activating PPARγ. In conclusion, this study highlights the advantage of LRPF to improve testicular development.

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