Transcriptome Analysis Revealed the Anabolic Regulation of Chlorophyll and Carotenoids in Curcuma alismatifolia Bracts.

Curcuma alismatifolia is an attractive ornamental plant in the ginger family. Its bracts come in a variety of colors and are commonly used as cut flowers, potted plants, and landscaping. To investigate the regulation of bract pigmentation in C. alismatifolia, we examined the pigment levels of chlorophyll and carotenoids in the pure color part (PC) and variegated part (VA) of three C. alismatifolia varieties, i.e., "Siam TM Sitrone," "Chiang Mai Pink," and "Snow White." To mine the color mechanisms of the pure color and variegated parts of the bract, we conducted RNA-seq analysis on C. alismatifolia. We identified a total of 89,975 unigenes, and there were 3584 differentially expressed genes identified post-screening. Furthermore, 1858 DEGs were annotated in the GO database and 681 in the KEGG database. We pinpointed key genes responsible for the diverse bract colors in C. alismatifolia, including ZEP for carotenoid synthesis and GAGA2 in the chlorophyll synthesis pathway. This study provides valuable insights into understanding the pigmentation mechanism of bracts in C. alismatifolia and the breeding process.