基于负电荷金纳米棒与 Ru(bpy)32 之间共振能量转移调节的有机磷农药电化学发光传感器。

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL Talanta Pub Date : 2025-01-01 Epub Date: 2024-09-23 DOI:10.1016/j.talanta.2024.126934

Zixin Li, Zeyu Lin, Lifen Chen, Yue Lin, Fang Luo, Cuiying Lin, Jian Wang, Bin Qiu, Zhenyu Lin

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引用次数: 0

摘要

结合带负电荷的金纳米棒（AuNRs）（作为受体）和Ru(bpy)32+（作为供体）的静电作用，构建了一种电化学发光共振能量转移（ECL-RET）传感器，并将其应用于有机磷农药（OPs）的检测。首先用聚苯乙烯磺酸盐（PSS）修饰 AuNRs，合成了带负电荷的 AuNRs，PSS 可以通过静电作用与 Ru(bpy)32+ 结合，从而使发光体被受体吸收，发生共振能量转移，只检测到较低的 ECL 信号。乙酰胆碱酯酶（AChE）可通过乙酰硫代胆碱（ATCH）的水解过程产生硫代胆碱，硫代胆碱可通过金硫作用与 PSS 修饰的 AuNRs（PSS-AuNRs）结合，从而使吸附的 Ru(bpy)32+ 释放到溶液中，导致 ECL 强度恢复。但 OPs 会抑制 AChE 的活性，从而抑制 ECL 信号的恢复过程。本研究选择毒死蜱作为模型目标物，结果表明 ECL 强度与毒死蜱浓度的对数之间的相关性在 1 ng/mL 至 1 mg/mL 之间呈显著的线性关系，检测限为 0.51 ng/mL。该系统已被用于检测实际样品中的 OPs，结果令人满意。

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Electrochemiluminescence sensor for organophosphorus pesticides based on the regulation of resonance energy transfer between negative charged gold nanorods and Ru(bpy)₃².

Combined the electrostatic interaction of the negatively charged gold nanorods (AuNRs) (as acceptor) and Ru(bpy)₃²⁺ (as donor), an electrochemiluminescence resonance energy transfer (ECL-RET) sensor was constructed and applied for the detection of organophosphorus pesticides (OPs). Negatively charged AuNRs were synthesized by modifying AuNRs with polystyrene sulfonate (PSS) firstly, which can bind to Ru(bpy)₃²⁺ through electrostatic interaction so that the luminophore was absorbed by the acceptor, the resonance energy transfer occurred and only low ECL signal had been detected. Thiocholine can be produced by the hydrolysis process of acetylthiocholine (ATCh) with the help of acetylcholinesterase (AChE), which can bond with PSS-modified AuNRs (PSS-AuNRs) through gold-sulfur interaction, this caused the releasing of the adsorbed Ru(bpy)₃²⁺ into the solution and resulting in the restoration of the ECL intensity. However, the activity of AChE was inhibited by OPs, and the recovery process of the ECL signal was thus suppressed as well. In this study, chlorpyrifos was chosen as model target, the results indicated that the correlation between the ECL intensity and the logarithm of chlorpyrifos concentration showed remarkable linearity across 1 ng/mL to 1 mg/mL, achieving a detection limit of 0.51 ng/mL. The proposed system has been utilized for detecting OPs in real samples with satisfied results.

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.