人类单个骨骼肌纤维对高强度间歇运动的转录组特征。

IF 5 2区 生物学 Q2 CELL BIOLOGY American journal of physiology. Cell physiology Pub Date : 2024-11-01 Epub Date: 2024-09-24 DOI:10.1152/ajpcell.00299.2024
Thibaux Van der Stede, Alexia Van de Loock, Eline Lievens, Nurten Yigit, Jasper Anckaert, Ruud Van Thienen, Anneleen Weyns, Pieter Mestdagh, Jo Vandesompele, Wim Derave
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引用次数: 0

摘要

由于骨骼肌的纤维类型组成不尽相同,因此破译驱动运动对健康和表现产生益处的分子信号事件具有挑战性。我们开发了一种优化的工作流程,用于在高强度间歇性自行车运动之前、之后和恢复三小时之后对单个人体肌肉纤维进行转录谱分析。从转录的角度来看,我们发现纤维激活并不存在二分法,即纤维被招募或未被招募。相反,在运动后的恢复过程中,激活模式呈现出连续性,快速纤维与慢速纤维的反应更为一致。运动后立即出现的全转录组反应在慢纤维和快纤维中表现出一些不同的特征,尽管对运动反应最强烈的基因在两种纤维类型中是共通的。时间转录波使两种纤维类型的基因特征在运动恢复期间进一步趋于相似。此外,还观察到所有静息和运动纤维之间存在巨大的异质性,主要驱动因素与慢/快类型无关。除了基于肌球蛋白重链的分类外,这种深刻的异质性还延伸到纤维的不同运动反应。总之,我们的单纤维方法表明,肌肉纤维对高强度间歇运动的反应存在着巨大的纤维间多样性。
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Transcriptomic signatures of human single skeletal muscle fibers in response to high-intensity interval exercise.

The heterogeneous fiber type composition of skeletal muscle makes it challenging to decipher the molecular signaling events driving the health- and performance benefits of exercise. We developed an optimized workflow for transcriptional profiling of individual human muscle fibers before, immediately after, and after 3 h of recovery from high-intensity interval cycling exercise. From a transcriptional point-of-view, we observe that there is no dichotomy in fiber activation, which could refer to a fiber being recruited or nonrecruited. Rather, the activation pattern displays a continuum with a more uniform response within fast versus slow fibers during the recovery from exercise. The transcriptome-wide response immediately after exercise is characterized by some distinct signatures for slow versus fast fibers, although the most exercise-responsive genes are common between the two fiber types. The temporal transcriptional waves further converge the gene signatures of both fiber types toward a more similar profile during the recovery from exercise. Furthermore, a large heterogeneity among all resting and exercised fibers was observed, with the principal drivers being independent of a slow/fast typology. This profound heterogeneity extends to distinct exercise responses of fibers beyond a classification based on myosin heavy chains. Collectively, our single-fiber methodological approach points to a substantial between-fiber diversity in muscle fiber responses to high-intensity interval exercise.NEW & NOTEWORTHY By development of a single-fiber transcriptomics technology, we assessed the transcriptional events in individual human skeletal muscle fibers upon high-intensity exercise. We demonstrate a large variability in transcriptional activation of fibers, with shared and distinct gene signatures for slow and fast fibers. The heterogeneous fiber-specific exercise response extends beyond this traditional slow/fast categorization. These findings expand on our understanding of exercise responses and uncover a profound between-fiber diversity in muscle fiber activation and transcriptional perturbations.

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来源期刊
CiteScore
9.10
自引率
1.80%
发文量
252
审稿时长
1 months
期刊介绍: The American Journal of Physiology-Cell Physiology is dedicated to innovative approaches to the study of cell and molecular physiology. Contributions that use cellular and molecular approaches to shed light on mechanisms of physiological control at higher levels of organization also appear regularly. Manuscripts dealing with the structure and function of cell membranes, contractile systems, cellular organelles, and membrane channels, transporters, and pumps are encouraged. Studies dealing with integrated regulation of cellular function, including mechanisms of signal transduction, development, gene expression, cell-to-cell interactions, and the cell physiology of pathophysiological states, are also eagerly sought. Interdisciplinary studies that apply the approaches of biochemistry, biophysics, molecular biology, morphology, and immunology to the determination of new principles in cell physiology are especially welcome.
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