通过空间转录组分析识别乳腺癌患者的高复发风险

IF 1.6 4区 医学 Q4 ONCOLOGY Anticancer research Pub Date : 2024-10-01 DOI:10.21873/anticanres.17268
Jinah Chu, Sung-Im DO, Hyun-Soo Kim
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引用次数: 0

摘要

背景/目的:利用空间分辨转录组分析比较复发风险的基因表达谱尚未见报道。本研究旨在确定与高Oncotype DX复发评分(ORS)相关的乳腺癌的不同遗传特征:将患者分为两组:ORS-高(ORS-H;两名患者)和ORS-非高(ORS-NH;五名患者)。我们进行了数字空间图谱分析和生物信息学分析,以研究空间转录组图谱:结果:溶菌酶(LYZ)、补体C1q C链(C1QC)和补体C1q B链(C1QB)在ORS-H组基质区的变化倍数最高。对 ORS-H 组进行的基因本体富集分析显示,基质区与免疫反应相关的基因显著上调,包括淋巴细胞介导的免疫、与免疫球蛋白超家族相关的适应性免疫反应和白细胞介导的免疫。基因组富集分析表明,基质区与干扰素(IFN)反应和补体途径相关的基因组明显正富集:本研究强调了ORS-H和ORS-NH乳腺癌在基因表达谱和空间分辨转录活性方面的显著差异。在ORS-H组的基质区,与细胞介导免疫、IFN反应和补体C1q相关的基因和通路明显上调,这值得在更大的人群队列中进行进一步评估。
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Identifying High Recurrence Risk in Breast Carcinoma Patients Through Spatial Transcriptomic Analysis.

Background/aim: Comparing gene expression profiles according to recurrence risk using spatially resolved transcriptomic analysis has not been reported. This study aimed to identify distinct genetic features of breast carcinoma associated with a high Oncotype DX Recurrence Score (ORS).

Patients and methods: Patients were categorized into two groups, ORS-high (ORS-H; two patients) and ORS-non-high (ORS-NH; five patients). We performed digital spatial profiling and bioinformatic analyses to investigate the spatial transcriptomic profiles.

Results: Lysozyme (LYZ), complement C1q C chain (C1QC), and complement C1q B chain (C1QB) exhibited the highest fold changes in the stromal compartment of the ORS-H group. Gene ontology enrichment analysis of the ORS-H group revealed significant up-regulation of genes associated with immune response in the stromal compartment, including lymphocyte-mediated immunity, adaptive immune response related to the immunoglobulin superfamily, and leukocyte-mediated immunity. Gene set enrichment analysis showed significant positive enrichment of gene sets associated with interferon (IFN) response and complement pathways in the stromal compartment.

Conclusion: This study highlights significant differences in gene expression profiles and spatially resolved transcriptional activities between ORS-H and ORS-NH breast carcinomas. The significant up-regulation of genes and pathways associated with cell-mediated immunity, IFN response, and complement C1q in the stromal compartment of the ORS-H group warrants further evaluation with larger population cohorts.

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来源期刊
Anticancer research
Anticancer research 医学-肿瘤学
CiteScore
3.70
自引率
10.00%
发文量
566
审稿时长
2 months
期刊介绍: ANTICANCER RESEARCH is an independent international peer-reviewed journal devoted to the rapid publication of high quality original articles and reviews on all aspects of experimental and clinical oncology. Prompt evaluation of all submitted articles in confidence and rapid publication within 1-2 months of acceptance are guaranteed. ANTICANCER RESEARCH was established in 1981 and is published monthly (bimonthly until the end of 2008). Each annual volume contains twelve issues and index. Each issue may be divided into three parts (A: Reviews, B: Experimental studies, and C: Clinical and Epidemiological studies). Special issues, presenting the proceedings of meetings or groups of papers on topics of significant progress, will also be included in each volume. There is no limitation to the number of pages per issue.
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