PDIA4 在肺腺癌中的潜在抗肿瘤特性

IF 1.6 4区 医学 Q4 ONCOLOGY Anticancer research Pub Date : 2024-10-01 DOI:10.21873/anticanres.17260
Hyeon Ji Kim, DO-Yeon Kim
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引用次数: 0

摘要

背景/目的:鉴于肺癌的高发病率和高死亡率,人们开展了各种分子研究,以了解癌症的病理生理学并开发新的治疗策略。参与蛋白质组装和内质网平衡的 PDIA4 基因在各种肺癌亚型中都有过表达。然而,它在肺腺癌(LUAD)中的确切功能仍不明确。本研究旨在研究 PDIA4 在 LUAD 中的作用,并探索其作为双试剂基因的作用:采用 Crispr/Cas9 技术敲除 A549 和 LA-4 肺腺瘤细胞中 PDIA4 的表达。结果:PDIA4 基因缺陷导致 A549 和 LA-4 肺腺瘤细胞的生长、迁移和凋亡增加:结果:PDIA4缺陷导致A549和LA-4肺癌细胞的生长速度加快、迁移能力增强、对凋亡的抵抗力增强。从机理上讲,氧化应激的上调以及 NF-[式中:见正文]B的活化可能是导致 PDIA4 沉默后出现肿瘤促进效应的原因:结论:PDIA4在肺腺癌中似乎具有肿瘤抑制因子的功能,这表明PDIA4可能是一种双效基因,根据不同的情况既能抑制肿瘤,也能促进肿瘤生长。
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Potential Anti-tumor Properties of PDIA4 in Lung Adenocarcinoma.

Background/aim: Given the high frequency and mortality rate of lung cancer, diverse molecular studies have been undertaken to understand cancer pathophysiology and develop novel treatment strategies. The PDIA4 gene, which is involved in protein assembly and endoplasmic reticulum homeostasis, is overexpressed in various lung cancer subtypes. However, its exact function in lung adenocarcinoma (LUAD) remains elusive. The study aimed to investigate the role of PDIA4 in LUAD and explore its role as double-agent gene.

Materials and methods: PDIA4 expression was knocked out in A549 and LA-4 lung adenoma cells using the Crispr/Cas9 technology. Cell growth, migration, and apoptosis were analyzed in control and PDIA4-deficient cells.

Results: PDIA4 deficiency resulted in increased cell growth, enhanced migration capacity, and greater resistance to apoptosis in both A549 and LA-4 lung cancer cells. Mechanistically, up-regulation of oxidative stress followed by NF-[Formula: see text]B activation may contribute to tumor-promoting effects observed upon PDIA4 silencing.

Conclusion: PDIA4 appears to function as a tumor suppressor in lung adenocarcinoma, suggesting that PDIA4 may act as a double-agent gene, with roles both on tumor suppression and promotion depending on the context.

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来源期刊
Anticancer research
Anticancer research 医学-肿瘤学
CiteScore
3.70
自引率
10.00%
发文量
566
审稿时长
2 months
期刊介绍: ANTICANCER RESEARCH is an independent international peer-reviewed journal devoted to the rapid publication of high quality original articles and reviews on all aspects of experimental and clinical oncology. Prompt evaluation of all submitted articles in confidence and rapid publication within 1-2 months of acceptance are guaranteed. ANTICANCER RESEARCH was established in 1981 and is published monthly (bimonthly until the end of 2008). Each annual volume contains twelve issues and index. Each issue may be divided into three parts (A: Reviews, B: Experimental studies, and C: Clinical and Epidemiological studies). Special issues, presenting the proceedings of meetings or groups of papers on topics of significant progress, will also be included in each volume. There is no limitation to the number of pages per issue.
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