早期胃癌淋巴结转移的预测性生物标志物:临床病理特征、蛋白表达、Epstein-Barr 病毒状态和微卫星不稳定性的参考文献

IF 1.6 4区 医学 Q4 ONCOLOGY Anticancer research Pub Date : 2024-10-01 DOI:10.21873/anticanres.17273
Sun-Ju Byeon, Mee Soo Chang, Hye Eun Park, Doehee Kang, Yuting Wang, Dong-Seok Han, Hye Sung Ahn, Seung Chul Heo, Myong Seok Lee, Won Kim, Su Hwan Kim, Dong-Won Ahn, Kook Lae Lee
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引用次数: 0

摘要

背景/目的:预测早期胃癌(EGC)的淋巴结转移(LNM)对治疗决策至关重要。本研究旨在确认淋巴结转移的风险因素,并确定预测EGC淋巴结转移的新型辅助生物标志物:我们建立了一个训练集,其中包括 63 名 LNM-EGC 患者和 274 名非 LNM EGC 患者;还建立了一个测试集,其中包括 19 名 LNM-EGC 患者和 146 名非 LNM EGC 患者。对淋巴管生成和相关通路成分(VEGF-C、TGF-β1、SMAD2/3、VEGF-D、pSTAT3、E-cadherin、CD44、c-MET、YAP和HER2)进行了免疫组化,对Epstein-Barr病毒编码的小RNA进行了原位杂交,并对微卫星不稳定性进行了多重PCR检测:结果:在训练集中,劳伦的弥漫/混合分类、基质脱落、粘膜下浸润≥500 μm、淋巴浸润、VEGF-C和SMAD2/3高表达是LNM的独立危险因素(p结论:基质脱落、粘膜下浸润≥500 μm、淋巴浸润、VEGF-C和SMAD2/3高表达是LNM的独立危险因素:基质脱落、粘膜下侵袭和 VEGF-C 高表达是 EGC LNM 的潜在生物标志物。VEGF-C的表达可作为辅助生物标志物,用于预测初诊时钳取活检组织中的LNM。
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Predictive Biomarkers of Lymph Node Metastasis in Early Gastric Cancer: A Reference of Clinicopathological Characteristics, Protein Expression, Epstein-Barr Virus Status, and Microsatellite Instability.

Background/aim: Predicting lymph node metastasis (LNM) in early gastric cancer (EGC) is crucial for making treatment decisions. This study aimed to confirm risk factors for LNM and identify novel auxiliary biomarkers for predicting LNM in EGC.

Patients and methods: We established a training set, comprising 63 patients with LNM-EGC and 274 patients with non-LNM EGC, and a test set, comprising 19 patients with LNM-EGC and 146 non-LNM EGC. Immunohistochemistry for lymphangiogenic and related pathway components (VEGF-C, TGF-β1, SMAD2/3, VEGF-D, pSTAT3, E-cadherin, CD44, c-MET, YAP, and HER2), in situ hybridization for Epstein-Barr virus-encoded small RNAs, and multiplex PCR for microsatellite instability were conducted.

Results: In the training set, Lauren's diffuse/mixed classification, stromal desmoplasia, submucosal invasion ≥500 μm, lymphatic invasion, and high VEGF-C and SMAD2/3 expression were independent risk factors for LNM (p<0.05). A large tumor size, mixed histology, submucosal invasion, perineural invasion, and ulceration were determined as risk factors using univariate analysis (p<0.05). The tumor cutoff size for predicting LNM was 2.65 cm, based on a ROC analysis. The test set study verified that stromal desmoplasia, submucosal invasion, and high VEGF-C expression were independent risk factors for LNM (p<0.05). Moreover, mixed histology, lymphatic invasion, ulceration, and high SMAD 2/3 expression were identified as additional risk factors using univariate analysis (p<0.05).

Conclusion: Stromal desmoplasia, submucosal invasion, and high VEGF-C expression are potential biomarkers for LNM in EGC. VEGF-C expression might serve as an adjunct biomarker for predicting LNM on forceps-biopsy tissue at initial diagnosis.

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来源期刊
Anticancer research
Anticancer research 医学-肿瘤学
CiteScore
3.70
自引率
10.00%
发文量
566
审稿时长
2 months
期刊介绍: ANTICANCER RESEARCH is an independent international peer-reviewed journal devoted to the rapid publication of high quality original articles and reviews on all aspects of experimental and clinical oncology. Prompt evaluation of all submitted articles in confidence and rapid publication within 1-2 months of acceptance are guaranteed. ANTICANCER RESEARCH was established in 1981 and is published monthly (bimonthly until the end of 2008). Each annual volume contains twelve issues and index. Each issue may be divided into three parts (A: Reviews, B: Experimental studies, and C: Clinical and Epidemiological studies). Special issues, presenting the proceedings of meetings or groups of papers on topics of significant progress, will also be included in each volume. There is no limitation to the number of pages per issue.
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