血清 CD5L 作为妊娠期甲状腺激素状态的潜在生物标志物。

IF 5 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY BioFactors Pub Date : 2024-09-30 DOI:10.1002/biof.2123
Sabrina Asaad, Thilo Samson Chillon, Dorota Filipowicz, Britta Wilms, Frank Strenge, Ewelina Szczepanek-Parulska, Waldemar B Minich, Sebastian M Meyhöfer, Jens U Marquardt, Jens Mittag, Henrik Oster, Marek Ruchala, Lutz Schomburg
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引用次数: 0

摘要

甲状腺激素(TH)状态通常通过促甲状腺激素(TSH)和甲状腺素(T4)进行评估。这两种生物标志物主要受 TH 受体 beta 的调节,而许多外周器官则使用 alpha 受体。血清分化簇 5 样分子(CD5L)是一种来源于肝脏的蛋白质,受两种 TH 受体异构体的控制。然而,有关其与 TH 状态关系的临床数据却很少。尤其是在妊娠期间,常规生物标志物会受到动态干扰,因此还需要一种额外的生物标志物来反映 TH 状态。本研究旨在确定调节血清 CD5L 的可能协变量,并测试其作为孕期 TH 附加生物标志物的潜在适用性。研究人员使用新研制的抗体建立了血清 CD5L 的夹心 ELISA 方法。评估了昼夜节律效应和肝病对血清 CD5L 浓度的影响。对TH和微量元素状态特征明确的孕妇血清样本进行了分析,CD5L浓度与TH状态的其他指标(包括促甲状腺激素、fT4、fT3、铜和硒浓度)相关。新的 CD5L 定量检测方法显示出很高的准确性。血清 CD5L 在稀释和再冷冻实验中表现稳定,没有表现出强烈的昼夜节律变化,也不依赖于肝脏疾病。在孕妇血清中,CD5L 与 fT3 呈正相关,但与 fT4 或 TSH 无关。CD5L与血清中对TH有反应的微量元素硒和铜的水平呈显著正相关。这些数据支持将血清 CD5L 作为额外的 TH 状态标记物的潜在适用性,它对妊娠和甲状腺疾病具有潜在价值。
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Serum CD5L as potential biomarker of thyroid hormone status during pregnancy.

The thyroid hormone (TH) status is routinely assessed by thyrotropin (TSH) and thyroxine (T4). Both biomarkers are mainly regulated by TH receptor beta, whereas many peripheral organs employ the alpha receptor. Serum cluster of differentiation 5-like molecule (CD5L) is a liver-derived protein under control of both TH receptor isoforms. However, clinical data on its relation to TH status are sparse. An additional biomarker of TH status is needed in particular during pregnancy, where the routine biomarkers become dynamically disturbed. This study aimed to determine possible covariates regulating serum CD5L and to test its potential suitability as additional TH biomarker during pregnancy. A sandwich ELISA for serum CD5L was established using newly raised antibodies. Circadian effects and the impact of liver disease on serum CD5L concentrations were assessed. Serum samples from pregnant women with well-characterized TH and trace element status were analyzed, and CD5L concentrations were correlated with other indicators of TH status including TSH, fT4, fT3, copper, and selenium concentrations. The new quantitative assay for CD5L showed high accuracy. Serum CD5L was stable in dilution and refreezing experiments and did not show strong circadian variance or dependency on liver disease. In serum of pregnant women, CD5L correlated positively to fT3, but not to fT4 or TSH. Significant positive correlations of CD5L were observed with serum levels of the TH-responsive trace elements selenium and copper. The data support the potential suitability of serum CD5L as an additional marker of TH status, with potential value for pregnancy and thyroid disease.

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来源期刊
BioFactors
BioFactors 生物-内分泌学与代谢
CiteScore
11.50
自引率
3.30%
发文量
96
审稿时长
6-12 weeks
期刊介绍: BioFactors, a journal of the International Union of Biochemistry and Molecular Biology, is devoted to the rapid publication of highly significant original research articles and reviews in experimental biology in health and disease. The word “biofactors” refers to the many compounds that regulate biological functions. Biological factors comprise many molecules produced or modified by living organisms, and present in many essential systems like the blood, the nervous or immunological systems. A non-exhaustive list of biological factors includes neurotransmitters, cytokines, chemokines, hormones, coagulation factors, transcription factors, signaling molecules, receptor ligands and many more. In the group of biofactors we can accommodate several classical molecules not synthetized in the body such as vitamins, micronutrients or essential trace elements. In keeping with this unified view of biochemistry, BioFactors publishes research dealing with the identification of new substances and the elucidation of their functions at the biophysical, biochemical, cellular and human level as well as studies revealing novel functions of already known biofactors. The journal encourages the submission of studies that use biochemistry, biophysics, cell and molecular biology and/or cell signaling approaches.
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