{"title":"胆汁蛋白质组学和代谢组学分析的整合揭示了高海拔地区不同类型胆结石的新见解。","authors":"Xiaofeng Jing, Ying Ma, Defu Li, Tiecheng Zhang, Haiqi Xiang, Fan Xu, Yonghong Xia","doi":"10.1186/s12876-024-03422-5","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>To explore the pathogenesis of different subtypes of gallstones in high-altitude populations from a molecular perspective.</p><p><strong>Methods: </strong>We collected bile samples from 20 cholesterol gallstone disease (CGD) patients and 20 pigment gallstone disease (PGD) patients. Proteomics analysis was performed by LC/MS DIA, while metabolomics analysis was performed by UPLC- Q-TOF/MS.</p><p><strong>Results: </strong>We identified 154 up-regulated and 196 down-regulated differentially expressed proteins, which were significantly enriched in neurodegenerative diseases, energy metabolism, amino acid metabolism etc. In metabolomics analysis, 20 up-regulated and 63 down-regulated differentially expressed metabolites were identified, and they were significantly enriched in vitamin B6 metabolism. Three pathways of integrated proteomics and metabolomics were significantly enriched: porphyrin and chlorophyll metabolism, riboflavin metabolism and aminoacyl-tRNA biosynthesis. Remarkably, 7 differentially expressed proteins and metabolites showed excellent predictive performance and were selected as potential biomarkers.</p><p><strong>Conclusion: </strong>The findings of our metabolomics and proteomics analyses help to elucidate the underlying mechanisms of gallstone formation in high-altitude populations.</p>","PeriodicalId":9129,"journal":{"name":"BMC Gastroenterology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Integration of bile proteomics and metabolomics analyses reveals novel insights into different types of gallstones in a high-altitude area.\",\"authors\":\"Xiaofeng Jing, Ying Ma, Defu Li, Tiecheng Zhang, Haiqi Xiang, Fan Xu, Yonghong Xia\",\"doi\":\"10.1186/s12876-024-03422-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>To explore the pathogenesis of different subtypes of gallstones in high-altitude populations from a molecular perspective.</p><p><strong>Methods: </strong>We collected bile samples from 20 cholesterol gallstone disease (CGD) patients and 20 pigment gallstone disease (PGD) patients. Proteomics analysis was performed by LC/MS DIA, while metabolomics analysis was performed by UPLC- Q-TOF/MS.</p><p><strong>Results: </strong>We identified 154 up-regulated and 196 down-regulated differentially expressed proteins, which were significantly enriched in neurodegenerative diseases, energy metabolism, amino acid metabolism etc. In metabolomics analysis, 20 up-regulated and 63 down-regulated differentially expressed metabolites were identified, and they were significantly enriched in vitamin B6 metabolism. Three pathways of integrated proteomics and metabolomics were significantly enriched: porphyrin and chlorophyll metabolism, riboflavin metabolism and aminoacyl-tRNA biosynthesis. Remarkably, 7 differentially expressed proteins and metabolites showed excellent predictive performance and were selected as potential biomarkers.</p><p><strong>Conclusion: </strong>The findings of our metabolomics and proteomics analyses help to elucidate the underlying mechanisms of gallstone formation in high-altitude populations.</p>\",\"PeriodicalId\":9129,\"journal\":{\"name\":\"BMC Gastroenterology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-09-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BMC Gastroenterology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1186/s12876-024-03422-5\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"GASTROENTEROLOGY & HEPATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Gastroenterology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s12876-024-03422-5","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"GASTROENTEROLOGY & HEPATOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
背景:从分子角度探讨高海拔人群中不同亚型胆结石的发病机制:从分子角度探讨高海拔人群中不同亚型胆结石的发病机制:我们采集了20名胆固醇性胆石症(CGD)患者和20名色素性胆石症(PGD)患者的胆汁样本。采用 LC/MS DIA 进行蛋白质组学分析,采用 UPLC- Q-TOF/MS 进行代谢组学分析:结果:我们发现了154个上调和196个下调的差异表达蛋白,这些蛋白在神经退行性疾病、能量代谢、氨基酸代谢等方面有明显的富集。在代谢组学分析中,发现了20个上调和63个下调的差异表达代谢物,它们在维生素B6代谢中明显富集。综合蛋白质组学和代谢组学研究发现,卟啉和叶绿素代谢、核黄素代谢和氨基酰-tRNA生物合成这三个途径的表达明显富集。值得注意的是,有 7 种差异表达的蛋白质和代谢物显示出极佳的预测性能,并被选为潜在的生物标记物:我们的代谢组学和蛋白质组学分析结果有助于阐明高海拔人群胆石形成的潜在机制。
Integration of bile proteomics and metabolomics analyses reveals novel insights into different types of gallstones in a high-altitude area.
Background: To explore the pathogenesis of different subtypes of gallstones in high-altitude populations from a molecular perspective.
Methods: We collected bile samples from 20 cholesterol gallstone disease (CGD) patients and 20 pigment gallstone disease (PGD) patients. Proteomics analysis was performed by LC/MS DIA, while metabolomics analysis was performed by UPLC- Q-TOF/MS.
Results: We identified 154 up-regulated and 196 down-regulated differentially expressed proteins, which were significantly enriched in neurodegenerative diseases, energy metabolism, amino acid metabolism etc. In metabolomics analysis, 20 up-regulated and 63 down-regulated differentially expressed metabolites were identified, and they were significantly enriched in vitamin B6 metabolism. Three pathways of integrated proteomics and metabolomics were significantly enriched: porphyrin and chlorophyll metabolism, riboflavin metabolism and aminoacyl-tRNA biosynthesis. Remarkably, 7 differentially expressed proteins and metabolites showed excellent predictive performance and were selected as potential biomarkers.
Conclusion: The findings of our metabolomics and proteomics analyses help to elucidate the underlying mechanisms of gallstone formation in high-altitude populations.
期刊介绍:
BMC Gastroenterology is an open access, peer-reviewed journal that considers articles on all aspects of the prevention, diagnosis and management of gastrointestinal and hepatobiliary disorders, as well as related molecular genetics, pathophysiology, and epidemiology.