实验性自身免疫性脑脊髓炎小鼠脊髓神经元中巨噬细胞集落刺激因子 CSF-1 的表达

IF 0.9 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-01 Epub Date: 2024-10-01 DOI:10.1007/s10517-024-06249-y

A V Balashov, V P Balashov, N P Shikhanov, S V Gushchina

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引用次数: 0

摘要

在急性和慢性实验性自身免疫性脑脊髓炎（EAE）模型小鼠中，用酶联免疫吸附法对脊髓和血浆中CSF-1和IL-34蛋白水平的动态进行了定量研究，并用免疫组织学方法检测了CSF-1在脊髓细胞中表达的特异性。在急性和慢性 EAE 小鼠中，脊髓中的 CSF-1 水平明显升高，并发现了具有强烈 CSF-1 免疫反应的运动神经元群。研究结果表明，CSF-1 在 EAE/多发性硬化症的发病机制和进展过程中可能发挥作用。

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Expression of Macrophage Colony-Stimulating Factor CSF-1 in Spinal Cord Neurons in Mice with Experimental Autoimmune Encephalomyelitis.

In mice with acute and chronic models of experimental autoimmune encephalomyelitis (EAE), a quantitative study of the dynamics of CSF-1 and IL-34 protein levels in the spinal cord and blood plasma was conducted by ELISA and the specificity of CSF-1 expression in spinal cord cells was examined by immunohistological methods. A significant increase in the level of CSF-1 in the spinal cord was detected and populations of motoneurons with intense CSF-1 immunoreactivity were identified in mice with acute and chronic EAE. The obtained results suggest a possible role of CSF-1 in the pathogenesis and progression of EAE/multiple sclerosis.

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.