性激素对有机阴离子转运多肽 1B3 (OATP1B3) 的调控。

IF 0.9 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-01 Epub Date: 2024-09-30 DOI:10.1007/s10517-024-06238-1

A A Slepnev, Yu V Abalenikhina, A V Shchulkin, P D Ananyeva, E N Yakusheva

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引用次数: 0

摘要

使用 HepG2 细胞研究了性激素对有机阴离子转运多肽 OATP1B3 的调控机制。向细胞中添加雌二醇、孕酮和睾酮，浓度分别为 1、10 和 100 μM，持续 24 小时。浓度为 10 和 100 μM 的雌二醇通过法尼类固醇 X 受体增加了 OATP1B3 的水平，浓度为 1、10 和 100 μM 的睾酮通过对肝 X 受体亚型 α（LXRα）的影响增加了转运蛋白的表达，而孕酮则不影响 OATP1B3 的表达。

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Regulation of the Organic Anion Transporting Polypeptide 1B3 (OATP1B3) by Sex Hormones.

The mechanisms of regulation of the organic anion transporting polypeptide OATP1B3 by sex hormones were studied using HepG2 cells. Estradiol, progesterone, and testosterone were added to cells at concentrations of 1, 10, 100 μM for 24 h. The relative content of OATP1B3 was evaluated by Western blotting. Estradiol at concentrations of 10 and 100 μM increased the level of OATP1B3 acting through the farnesoid X-receptor, testosterone at concentrations of 1, 10, and 100 μM increased the expression of the transporter protein due to its effect on the liver X-receptor subtype α (LXRα), and progesterone did not affect the expression of OATP1B3.

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.