探索纤维蛋白凝胶在促进细胞迁移以形成血管方面的作用

IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Journal of Functional Biomaterials Pub Date : 2024-09-12 DOI:10.3390/jfb15090265
Joana A Moura, Hugh J Barlow, Shareen H Doak, Karl Hawkins, Iris Muller, Martin J D Clift
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引用次数: 0

摘要

血管生成的一个标志是内皮细胞的萌发。要在体外复制这一过程,生物材料方法可在促进细胞迁移方面发挥重要作用。为了研究纤维蛋白支架(纤维蛋白原:凝血酶混合物)支持内皮细胞迁移的能力,我们测量了用 HULEC 细胞系形成的球形细胞的迁移面积。在将细胞包裹在纤维蛋白凝胶中之前,首先使用悬滴技术让细胞形成球形。然后在纤维蛋白凝胶中包埋两天后测量细胞的迁移面积。除了流变学、孔隙率和纤维厚度分析外,还对影响纤维蛋白凝胶聚合的各种条件(如不同浓度的纤维蛋白原和凝血酶)进行了评估,以了解这些因素如何影响细胞在复合生物材料中的行为。数据表明凝血酶在调节纤维蛋白凝胶聚合中的作用;浓度越高,凝胶硬度越低(损失正切在 0.07 和 0.034 之间),细胞迁移率越高(测试的最大浓度:5 U/mL)。本文介绍的方法可以更精确地确定纤维蛋白凝胶的交联条件,从而用于刺激血管新生萌芽。
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Exploring the Role of Fibrin Gels in Enhancing Cell Migration for Vasculature Formation.

A hallmark of angiogenesis is the sprouting of endothelial cells. To replicate this event in vitro, biomaterial approaches can play an essential role in promoting cell migration. To study the capacity of a scaffold of fibrin (fibrinogen:thrombin mix) to support the movement of the endothelial cells, the migration area of spheroids formed with the HULEC cell line was measured. The cells were first allowed to form a spheroid using the hanging drop technique before being encapsulated in the fibrin gel. The cells' migration area was then measured after two days of embedding in the fibrin gel. Various conditions affecting fibrin gel polymerization, such as different concentrations of fibrinogen and thrombin, were evaluated alongside rheology, porosity, and fiber thickness analysis to understand how these factors influenced cell behavior within the composite biomaterial. Data point toward thrombin's role in governing fibrin gel polymerization; higher concentrations result in less rigid gels (loss tangent between 0.07 and 0.034) and increased cell migration (maximum concentration tested: 5 U/mL). The herein presented method allows for a more precise determination of the crosslinking conditions of fibrin gel that can be used to stimulate angiogenic sprouting.

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来源期刊
Journal of Functional Biomaterials
Journal of Functional Biomaterials Engineering-Biomedical Engineering
CiteScore
4.60
自引率
4.20%
发文量
226
审稿时长
11 weeks
期刊介绍: Journal of Functional Biomaterials (JFB, ISSN 2079-4983) is an international and interdisciplinary scientific journal that publishes regular research papers (articles), reviews and short communications about applications of materials for biomedical use. JFB covers subjects from chemistry, pharmacy, biology, physics over to engineering. The journal focuses on the preparation, performance and use of functional biomaterials in biomedical devices and their behaviour in physiological environments. Our aim is to encourage scientists to publish their results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Several topical special issues will be published. Scope: adhesion, adsorption, biocompatibility, biohybrid materials, bio-inert materials, biomaterials, biomedical devices, biomimetic materials, bone repair, cardiovascular devices, ceramics, composite materials, dental implants, dental materials, drug delivery systems, functional biopolymers, glasses, hyper branched polymers, molecularly imprinted polymers (MIPs), nanomedicine, nanoparticles, nanotechnology, natural materials, self-assembly smart materials, stimuli responsive materials, surface modification, tissue devices, tissue engineering, tissue-derived materials, urological devices.
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