在不产生多羟基丁酸的突变株中发现膜囊泡的生物生成过程。

IF 2.1 4区环境科学与生态学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Microbes and Environments Pub Date : 2024-01-01 DOI:10.1264/jsme2.ME24007

Sangho Koh, Michio Sato, Hiromi Matsusaki, Seiichi Taguchi

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引用次数: 0

摘要

以前曾在大肠杆菌中检测到人工生产聚羟基丁酸（PHB）引起的胞外膜囊泡（MV）。我们在此观察了天然 PHB 生产者--坏死葡萄球菌 H16 的突变菌株（-PHB）的 MV 生物生成情况。通过对野生型菌株和突变型菌株进行比较电子显微镜分析，我们发现了这种反向关系。基于这些结果，我们推测 MV 生物生成与 PHB 生物合成之间存在生理关系。因此，我们认为 MV 的生物发生有可能成为一种发酵 "压力标记"，用于监控目标聚合物生产微生物平台的性能。

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The Discovery of Membrane Vesicle Biogenesis in the Polyhydroxybutyrate-non-producing Mutant Strain of Cupriavidus necator H16.

Extracellular membrane vesicles (MVs) caused by the artificial production of polyhydroxybutyrate (PHB) were previously detected in Escherichia coli. We herein observed MV biogenesis in the mutant strain (-PHB) of the natural PHB producer, Cupriavidus necator H16. This inverse relationship was revealed through comparative electron microscopic ana-lyses of wild-type and mutant strains. Based on these results, we speculate that a physiological relationship exists between MV biogenesis and PHB biosynthesis. Therefore, we propose the potential of MV biogenesis as a fermentative "stress marker" for monitoring the performance of target polymer-producing microbial platforms.

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来源期刊

Microbes and Environments 生物-生物工程与应用微生物

CiteScore

4.10

自引率

13.60%

发文量

审稿时长

3 months

期刊介绍： Microbial ecology in natural and engineered environments; Microbial degradation of xenobiotic compounds; Microbial processes in biogeochemical cycles; Microbial interactions and signaling with animals and plants; Interactions among microorganisms; Microorganisms related to public health; Phylogenetic and functional diversity of microbial communities; Genomics, metagenomics, and bioinformatics for microbiology; Application of microorganisms to agriculture, fishery, and industry; Molecular biology and biochemistry related to environmental microbiology; Methodology in general and environmental microbiology; Interdisciplinary research areas for microbial ecology (e.g., Astrobiology, and Origins of Life); Taxonomic description of novel microorganisms with ecological perspective; Physiology and metabolisms of microorganisms; Evolution of genes and microorganisms; Genome report of microorganisms with ecological perspective.