用于甲状腺新陈代谢研究的人体甲状腺类器官体外模型的开发和初步鉴定。

IF 3.8 3区 医学 Q2 CELL BIOLOGY Molecular and Cellular Endocrinology Pub Date : 2024-09-27 DOI:10.1016/j.mce.2024.112377
Sofia Cristiani , Andrea Bertolini , Vittoria Carnicelli , Lucia Contu , Valentina Vitelli , Alessandro Saba , Federica Saponaro , Grazia Chiellini , Antonietta Raffaella Maria Sabbatini , Maria Anita Giambelluca , Paola Lenzi , Francesco Fornai , Leonardo Rossi , Gabriele Materazzi , Carlo Enrico Ambrosini , Grazia Rutigliano , Riccardo Zucchi , Ranieri Bizzarri , Sandra Ghelardoni
{"title":"用于甲状腺新陈代谢研究的人体甲状腺类器官体外模型的开发和初步鉴定。","authors":"Sofia Cristiani ,&nbsp;Andrea Bertolini ,&nbsp;Vittoria Carnicelli ,&nbsp;Lucia Contu ,&nbsp;Valentina Vitelli ,&nbsp;Alessandro Saba ,&nbsp;Federica Saponaro ,&nbsp;Grazia Chiellini ,&nbsp;Antonietta Raffaella Maria Sabbatini ,&nbsp;Maria Anita Giambelluca ,&nbsp;Paola Lenzi ,&nbsp;Francesco Fornai ,&nbsp;Leonardo Rossi ,&nbsp;Gabriele Materazzi ,&nbsp;Carlo Enrico Ambrosini ,&nbsp;Grazia Rutigliano ,&nbsp;Riccardo Zucchi ,&nbsp;Ranieri Bizzarri ,&nbsp;Sandra Ghelardoni","doi":"10.1016/j.mce.2024.112377","DOIUrl":null,"url":null,"abstract":"<div><div>A 3D thyroid model was developed to address the limitations of 2D cultures and study the effects of compounds like 3-MNT on dehalogenase 1 (IYD) and metabolic activity. Morphology was assessed by TEM, and the expression of tissue-specific genes (<em>TPO</em>, <em>TSHR</em>, <em>PAX8</em>, <em>TTF-1</em>, <em>NIS</em>, <em>IYD</em>, <em>TG</em>) and metabolic features were analyzed using qRT-PCR, immunofluorescence, western blotting, ELISA, and LC-MS/MS, with and without TSH stimulus and 3-MNT treatment. Confocal and TEM analyses confirmed a follicle-like 3D structure. Expression of <em>TPO</em>, <em>NIS</em>, <em>TG</em>, <em>TSH</em>, and <em>PAX</em> markers was significantly higher (p &lt; 0.05) in 3D versus 2D cultures, and ELISA showed increased TG protein production. 3-MNT treatment inhibited IYD activity, indicated by increased MIT and DIT in the media, and significantly altered (p &lt; 0.05) <em>NIS</em>, <em>TG</em>, <em>IYD</em>, <em>TSHR</em>, and <em>TPO</em> expression. These findings suggest 3D thyroid cultures closely replicate tissue traits and functionality, providing a valuable tool for thyroid research.</div></div>","PeriodicalId":18707,"journal":{"name":"Molecular and Cellular Endocrinology","volume":"594 ","pages":"Article 112377"},"PeriodicalIF":3.8000,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development and primary characterization of a human thyroid organoid in vitro model for thyroid metabolism investigation\",\"authors\":\"Sofia Cristiani ,&nbsp;Andrea Bertolini ,&nbsp;Vittoria Carnicelli ,&nbsp;Lucia Contu ,&nbsp;Valentina Vitelli ,&nbsp;Alessandro Saba ,&nbsp;Federica Saponaro ,&nbsp;Grazia Chiellini ,&nbsp;Antonietta Raffaella Maria Sabbatini ,&nbsp;Maria Anita Giambelluca ,&nbsp;Paola Lenzi ,&nbsp;Francesco Fornai ,&nbsp;Leonardo Rossi ,&nbsp;Gabriele Materazzi ,&nbsp;Carlo Enrico Ambrosini ,&nbsp;Grazia Rutigliano ,&nbsp;Riccardo Zucchi ,&nbsp;Ranieri Bizzarri ,&nbsp;Sandra Ghelardoni\",\"doi\":\"10.1016/j.mce.2024.112377\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>A 3D thyroid model was developed to address the limitations of 2D cultures and study the effects of compounds like 3-MNT on dehalogenase 1 (IYD) and metabolic activity. Morphology was assessed by TEM, and the expression of tissue-specific genes (<em>TPO</em>, <em>TSHR</em>, <em>PAX8</em>, <em>TTF-1</em>, <em>NIS</em>, <em>IYD</em>, <em>TG</em>) and metabolic features were analyzed using qRT-PCR, immunofluorescence, western blotting, ELISA, and LC-MS/MS, with and without TSH stimulus and 3-MNT treatment. Confocal and TEM analyses confirmed a follicle-like 3D structure. Expression of <em>TPO</em>, <em>NIS</em>, <em>TG</em>, <em>TSH</em>, and <em>PAX</em> markers was significantly higher (p &lt; 0.05) in 3D versus 2D cultures, and ELISA showed increased TG protein production. 3-MNT treatment inhibited IYD activity, indicated by increased MIT and DIT in the media, and significantly altered (p &lt; 0.05) <em>NIS</em>, <em>TG</em>, <em>IYD</em>, <em>TSHR</em>, and <em>TPO</em> expression. These findings suggest 3D thyroid cultures closely replicate tissue traits and functionality, providing a valuable tool for thyroid research.</div></div>\",\"PeriodicalId\":18707,\"journal\":{\"name\":\"Molecular and Cellular Endocrinology\",\"volume\":\"594 \",\"pages\":\"Article 112377\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2024-09-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular and Cellular Endocrinology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0303720724002338\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular and Cellular Endocrinology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0303720724002338","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

为了解决二维培养的局限性,并研究 3-MNT 等化合物对脱卤酶 1(IYD)和代谢活动的影响,我们开发了一种三维甲状腺模型。在有或没有促甲状腺激素刺激和 3-MNT 处理的情况下,用 TEM 评估了形态学,并用 qRT-PCR、免疫荧光、Western 印迹、ELISA 和 LC-MS/MS 分析了组织特异性基因(TPO、TSHR、PAX8、TTF-1、NIS、IYD、TG)的表达和代谢特征。共聚焦和 TEM 分析证实了滤泡样三维结构。与二维培养物相比,三维培养物中 TPO、NIS、TG、TSH 和 PAX 标记物的表达量明显更高(p < 0.05),ELISA 显示 TG 蛋白生成量增加。3-MNT 处理可抑制 IYD 活性(表现为培养基中 MIT 和 DIT 的增加),并显著改变 NIS、TG、IYD、TSHR 和 TPO 的表达(p < 0.05)。这些研究结果表明,三维甲状腺培养物密切复制了组织的特征和功能,为甲状腺研究提供了一种有价值的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Development and primary characterization of a human thyroid organoid in vitro model for thyroid metabolism investigation
A 3D thyroid model was developed to address the limitations of 2D cultures and study the effects of compounds like 3-MNT on dehalogenase 1 (IYD) and metabolic activity. Morphology was assessed by TEM, and the expression of tissue-specific genes (TPO, TSHR, PAX8, TTF-1, NIS, IYD, TG) and metabolic features were analyzed using qRT-PCR, immunofluorescence, western blotting, ELISA, and LC-MS/MS, with and without TSH stimulus and 3-MNT treatment. Confocal and TEM analyses confirmed a follicle-like 3D structure. Expression of TPO, NIS, TG, TSH, and PAX markers was significantly higher (p < 0.05) in 3D versus 2D cultures, and ELISA showed increased TG protein production. 3-MNT treatment inhibited IYD activity, indicated by increased MIT and DIT in the media, and significantly altered (p < 0.05) NIS, TG, IYD, TSHR, and TPO expression. These findings suggest 3D thyroid cultures closely replicate tissue traits and functionality, providing a valuable tool for thyroid research.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Molecular and Cellular Endocrinology
Molecular and Cellular Endocrinology 医学-内分泌学与代谢
CiteScore
9.00
自引率
2.40%
发文量
174
审稿时长
42 days
期刊介绍: Molecular and Cellular Endocrinology was established in 1974 to meet the demand for integrated publication on all aspects related to the genetic and biochemical effects, synthesis and secretions of extracellular signals (hormones, neurotransmitters, etc.) and to the understanding of cellular regulatory mechanisms involved in hormonal control.
期刊最新文献
Editorial Board Luteal fibroblasts produce prostaglandins in response to IL1β in a MAPK-mediated manner Oridonin attenuates diabetic retinopathy progression by suppressing NLRP3 inflammasome pathway Molecular basis of photoinduced seasonal energy rheostasis in Japanese quail (Coturnix japonica) ACSL5 promotes lipid deposition and lipoapoptosis in proximal tubular epithelial cells of diabetic kidney disease
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1