Laura M. Bartos , Sebastian T. Kunte , Stephan Wagner , Philipp Beumers , Rebecca Schaefer , Artem Zatcepin , Yunlei Li , Maria Griessl , Leonie Hoermann , Karin Wind-Mark , Peter Bartenstein , Sabina Tahirovic , Sibylle Ziegler , Matthias Brendel , Johannes Gnörich
{"title":"星状胶质细胞葡萄糖摄取决定小鼠健康衰老过程中大脑 FDG-PET 改变和代谢连接。","authors":"Laura M. Bartos , Sebastian T. Kunte , Stephan Wagner , Philipp Beumers , Rebecca Schaefer , Artem Zatcepin , Yunlei Li , Maria Griessl , Leonie Hoermann , Karin Wind-Mark , Peter Bartenstein , Sabina Tahirovic , Sibylle Ziegler , Matthias Brendel , Johannes Gnörich","doi":"10.1016/j.neuroimage.2024.120860","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><div>2-Fluorodeoxyglucose-PET (FDG-PET) is a powerful tool to study glucose metabolism in mammalian brains, but cellular sources of glucose uptake and metabolic connectivity during aging are not yet understood.</div></div><div><h3>Methods</h3><div>Healthy wild-type mice of both sexes (2–21 months of age) received FDG-PET and cell sorting after in vivo tracer injection (scRadiotracing). FDG uptake per cell was quantified in isolated microglia, astrocytes and neurons. Cerebral FDG uptake and metabolic connectivity were determined by PET. A subset of mice received measurement of blood glucose levels to study associations with cellular FDG uptake during aging.</div></div><div><h3>Results</h3><div>Cerebral FDG-PET signals in healthy mice increased linearly with age. Cellular FDG uptake of neurons increased between 2 and 12 months of age, followed by a strong decrease towards late ages. Contrarily, FDG uptake in microglia and astrocytes exhibited a U-shaped function with respect to age, comprising the predominant cellular source of higher cerebral FDG uptake in the later stages. Metabolic connectivity was closely associated with the ratio of glucose uptake in astroglial cells relative to neurons. Cellular FDG uptake was not associated with blood glucose levels and increasing FDG brain uptake as a function of age was still observed after adjusting for blood glucose levels.</div></div><div><h3>Conclusion</h3><div>Trajectories of astroglial glucose uptake drive brain FDG-PET alterations and metabolic connectivity during aging.</div></div>","PeriodicalId":19299,"journal":{"name":"NeuroImage","volume":"300 ","pages":"Article 120860"},"PeriodicalIF":4.7000,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Astroglial glucose uptake determines brain FDG-PET alterations and metabolic connectivity during healthy aging in mice\",\"authors\":\"Laura M. Bartos , Sebastian T. Kunte , Stephan Wagner , Philipp Beumers , Rebecca Schaefer , Artem Zatcepin , Yunlei Li , Maria Griessl , Leonie Hoermann , Karin Wind-Mark , Peter Bartenstein , Sabina Tahirovic , Sibylle Ziegler , Matthias Brendel , Johannes Gnörich\",\"doi\":\"10.1016/j.neuroimage.2024.120860\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Purpose</h3><div>2-Fluorodeoxyglucose-PET (FDG-PET) is a powerful tool to study glucose metabolism in mammalian brains, but cellular sources of glucose uptake and metabolic connectivity during aging are not yet understood.</div></div><div><h3>Methods</h3><div>Healthy wild-type mice of both sexes (2–21 months of age) received FDG-PET and cell sorting after in vivo tracer injection (scRadiotracing). FDG uptake per cell was quantified in isolated microglia, astrocytes and neurons. Cerebral FDG uptake and metabolic connectivity were determined by PET. A subset of mice received measurement of blood glucose levels to study associations with cellular FDG uptake during aging.</div></div><div><h3>Results</h3><div>Cerebral FDG-PET signals in healthy mice increased linearly with age. Cellular FDG uptake of neurons increased between 2 and 12 months of age, followed by a strong decrease towards late ages. Contrarily, FDG uptake in microglia and astrocytes exhibited a U-shaped function with respect to age, comprising the predominant cellular source of higher cerebral FDG uptake in the later stages. Metabolic connectivity was closely associated with the ratio of glucose uptake in astroglial cells relative to neurons. Cellular FDG uptake was not associated with blood glucose levels and increasing FDG brain uptake as a function of age was still observed after adjusting for blood glucose levels.</div></div><div><h3>Conclusion</h3><div>Trajectories of astroglial glucose uptake drive brain FDG-PET alterations and metabolic connectivity during aging.</div></div>\",\"PeriodicalId\":19299,\"journal\":{\"name\":\"NeuroImage\",\"volume\":\"300 \",\"pages\":\"Article 120860\"},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2024-09-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"NeuroImage\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1053811924003574\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"NEUROIMAGING\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"NeuroImage","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1053811924003574","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"NEUROIMAGING","Score":null,"Total":0}
Astroglial glucose uptake determines brain FDG-PET alterations and metabolic connectivity during healthy aging in mice
Purpose
2-Fluorodeoxyglucose-PET (FDG-PET) is a powerful tool to study glucose metabolism in mammalian brains, but cellular sources of glucose uptake and metabolic connectivity during aging are not yet understood.
Methods
Healthy wild-type mice of both sexes (2–21 months of age) received FDG-PET and cell sorting after in vivo tracer injection (scRadiotracing). FDG uptake per cell was quantified in isolated microglia, astrocytes and neurons. Cerebral FDG uptake and metabolic connectivity were determined by PET. A subset of mice received measurement of blood glucose levels to study associations with cellular FDG uptake during aging.
Results
Cerebral FDG-PET signals in healthy mice increased linearly with age. Cellular FDG uptake of neurons increased between 2 and 12 months of age, followed by a strong decrease towards late ages. Contrarily, FDG uptake in microglia and astrocytes exhibited a U-shaped function with respect to age, comprising the predominant cellular source of higher cerebral FDG uptake in the later stages. Metabolic connectivity was closely associated with the ratio of glucose uptake in astroglial cells relative to neurons. Cellular FDG uptake was not associated with blood glucose levels and increasing FDG brain uptake as a function of age was still observed after adjusting for blood glucose levels.
Conclusion
Trajectories of astroglial glucose uptake drive brain FDG-PET alterations and metabolic connectivity during aging.
期刊介绍：
NeuroImage, a Journal of Brain Function provides a vehicle for communicating important advances in acquiring, analyzing, and modelling neuroimaging data and in applying these techniques to the study of structure-function and brain-behavior relationships. Though the emphasis is on the macroscopic level of human brain organization, meso-and microscopic neuroimaging across all species will be considered if informative for understanding the aforementioned relationships.
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