{"title":"以天然光敏剂为介导的抗菌光动力疗法减少丙烯酸义齿基托模型上的多菌种生物膜","authors":"Ali Shahi Ardakani, Stefano Benedicenti, Luca Solimei, Sima Shahabi, Shima Afrasiabi","doi":"10.3390/ph17091232","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>The aim of this study is to investigate the antimicrobial efficacy of antimicrobial photodynamic therapy (PDT) using natural photosensitizers (curcumin, riboflavin, and phycocyanin) and light-emitting diode (LED) irradiation against multispecies biofilms in an acrylic denture base model.</p><p><strong>Materials and methods: </strong>Forty-five acrylic specimens were fabricated using heat-curing acrylic resin. The specimens were then infected with a mixed culture of bacterial and fungal species (including <i>Streptococcus mutans</i>, <i>Streptococcus sanguinis</i>, <i>Candida albicans</i>, and <i>Candida glabrata</i>) for 4 days. The acrylic discs were divided into nine groups, with each group containing five discs: control, 0.2% chlorhexidine, 5.25% sodium hypochlorite, curcumin, riboflavin, phycocyanin alone or along with LED. After treatment, the number of colony-forming units (CFUs) per milliliter was counted. In addition, the extent of biofilm degradation was assessed using the crystal violet staining method and scanning electron microscopy.</p><p><strong>Results: </strong>All experimental groups exhibited a significant reduction in colony numbers for both bacterial and fungal species compared to the control (<i>p</i> < 0.001). The PDT groups exhibited a statistically significant reduction in colony counts for both bacteria and fungi compared to the photosensitizer-only groups.</p><p><strong>Conclusions: </strong>The results of this in vitro study show that PDT with natural photosensitizers and LED devices can effectively reduce the viability and eradicate the biofilm of microorganisms responsible for causing denture infections.</p>","PeriodicalId":20198,"journal":{"name":"Pharmaceuticals","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11435042/pdf/","citationCount":"0","resultStr":"{\"title\":\"Reduction of Multispecies Biofilms on an Acrylic Denture Base Model by Antimicrobial Photodynamic Therapy Mediated by Natural Photosensitizers.\",\"authors\":\"Ali Shahi Ardakani, Stefano Benedicenti, Luca Solimei, Sima Shahabi, Shima Afrasiabi\",\"doi\":\"10.3390/ph17091232\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>The aim of this study is to investigate the antimicrobial efficacy of antimicrobial photodynamic therapy (PDT) using natural photosensitizers (curcumin, riboflavin, and phycocyanin) and light-emitting diode (LED) irradiation against multispecies biofilms in an acrylic denture base model.</p><p><strong>Materials and methods: </strong>Forty-five acrylic specimens were fabricated using heat-curing acrylic resin. The specimens were then infected with a mixed culture of bacterial and fungal species (including <i>Streptococcus mutans</i>, <i>Streptococcus sanguinis</i>, <i>Candida albicans</i>, and <i>Candida glabrata</i>) for 4 days. The acrylic discs were divided into nine groups, with each group containing five discs: control, 0.2% chlorhexidine, 5.25% sodium hypochlorite, curcumin, riboflavin, phycocyanin alone or along with LED. After treatment, the number of colony-forming units (CFUs) per milliliter was counted. In addition, the extent of biofilm degradation was assessed using the crystal violet staining method and scanning electron microscopy.</p><p><strong>Results: </strong>All experimental groups exhibited a significant reduction in colony numbers for both bacterial and fungal species compared to the control (<i>p</i> < 0.001). The PDT groups exhibited a statistically significant reduction in colony counts for both bacteria and fungi compared to the photosensitizer-only groups.</p><p><strong>Conclusions: </strong>The results of this in vitro study show that PDT with natural photosensitizers and LED devices can effectively reduce the viability and eradicate the biofilm of microorganisms responsible for causing denture infections.</p>\",\"PeriodicalId\":20198,\"journal\":{\"name\":\"Pharmaceuticals\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-09-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11435042/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmaceuticals\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3390/ph17091232\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MEDICINAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmaceuticals","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3390/ph17091232","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
引用次数: 0
摘要
研究目的本研究旨在探讨使用天然光敏剂(姜黄素、核黄素和藻蓝蛋白)和发光二极管(LED)照射抗菌光动力疗法(PDT)对丙烯酸义齿基托模型中多菌种生物膜的抗菌效果:使用热固化丙烯酸树脂制作了 45 个丙烯酸试样。然后用细菌和真菌混合培养物(包括变异链球菌、血链球菌、白色念珠菌和光滑念珠菌)感染这些试样 4 天。丙烯酸盘被分为九组,每组包含五个盘:对照组、0.2% 洗必泰组、5.25% 次氯酸钠组、姜黄素组、核黄素组、单独或与 LED 一起使用的植物花青素组。处理后,计算每毫升菌落形成单位(CFU)的数量。此外,还使用水晶紫染色法和扫描电子显微镜评估了生物膜的降解程度:结果:与对照组相比,所有实验组的细菌和真菌菌落数都明显减少(p < 0.001)。与仅使用光敏剂的实验组相比,PDT 实验组的细菌和真菌菌落数均有明显减少:这项体外研究的结果表明,使用天然光敏剂和 LED 设备的光动力疗法可以有效降低导致义齿感染的微生物的存活率并根除生物膜。
Reduction of Multispecies Biofilms on an Acrylic Denture Base Model by Antimicrobial Photodynamic Therapy Mediated by Natural Photosensitizers.
Objectives: The aim of this study is to investigate the antimicrobial efficacy of antimicrobial photodynamic therapy (PDT) using natural photosensitizers (curcumin, riboflavin, and phycocyanin) and light-emitting diode (LED) irradiation against multispecies biofilms in an acrylic denture base model.
Materials and methods: Forty-five acrylic specimens were fabricated using heat-curing acrylic resin. The specimens were then infected with a mixed culture of bacterial and fungal species (including Streptococcus mutans, Streptococcus sanguinis, Candida albicans, and Candida glabrata) for 4 days. The acrylic discs were divided into nine groups, with each group containing five discs: control, 0.2% chlorhexidine, 5.25% sodium hypochlorite, curcumin, riboflavin, phycocyanin alone or along with LED. After treatment, the number of colony-forming units (CFUs) per milliliter was counted. In addition, the extent of biofilm degradation was assessed using the crystal violet staining method and scanning electron microscopy.
Results: All experimental groups exhibited a significant reduction in colony numbers for both bacterial and fungal species compared to the control (p < 0.001). The PDT groups exhibited a statistically significant reduction in colony counts for both bacteria and fungi compared to the photosensitizer-only groups.
Conclusions: The results of this in vitro study show that PDT with natural photosensitizers and LED devices can effectively reduce the viability and eradicate the biofilm of microorganisms responsible for causing denture infections.