冷血浆可改善咪喹莫特诱导的小鼠牛皮癣样皮肤炎症

IF 2 4区 医学 Q3 DERMATOLOGY Skin Research and Technology Pub Date : 2024-10-01 DOI:10.1111/srt.70071
Yu-Jin Kim, Beom Joon Kim, Joon Seok, Hye Sung Han, Kwang Ho Yoo, Sun Young Choi
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引用次数: 0

摘要

目的:冷血浆通过减少炎症反应和调节细胞因子的表达,在各种皮肤病应用中显示出疗效。因此,本研究旨在探讨冷血浆对银屑病的治疗效果:方法:在使用冷血浆的银屑病 HaCaT 细胞中,我们证实了参与银屑病形成的炎症细胞因子、MAPK 通路、细胞周期和细胞凋亡相关因子的表达。在银屑病样 BALB/c 小鼠模型中,我们目测了冷血浆处理对皮肤的影响。通过 qPCR、Western 印迹和免疫组化证实了银屑病相关因子的表达:结果:冷血浆减少了银屑病细胞系中的炎症细胞因子,包括 IL-17A、IL-23A、IL-24、IL-1β 和 TNF-α。它还能调节参与 MAPK 通路和细胞周期的因子。在牛皮癣样小鼠模型中,冷血浆可改善皮肤厚度、红斑、鳞屑和 PASI。此外,还观察到 INF-γ、IL-23 和 S100a7 等炎症细胞因子的减少,以及 MAPK 通路激活、细胞凋亡和其他牛皮癣相关因子的改善:通过体外和体内研究,我们的研究强调了冷血浆作为一种新型银屑病治疗方法的潜力。此外,冷血浆还可作为皮肤免疫疾病的辅助疗法。
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Cold Plasma Ameliorates Imiquimod-Induced Psoriasis-Like Skin Inflammation in Mice.

Objectives: Cold plasma has shown efficacy in various dermatological applications by reduces inflammatory responses and modulating cytokine expression. Therefore, this study aimed to investigate the therapeutic effects of cold plasma on psoriasis.

Methods: In psoriasis HaCaT cells with cold plasma, we confirmed the expression of inflammatory cytokines involved in psoriasis formation and MAPK pathway, cell cycle, and apoptosis-related factors. In psoriasis-like BALB/c mice model, the effects of cold plasma treatment on skin were visually assessed. The expression of psoriasis-related factors was confirmed through qPCR, Western blotting, and Immunohistochemistry.

Results: Cold plasma led to a reduction in inflammatory cytokines including IL-17A, IL-23A, IL-24, IL-1β, and TNF-α in the psoriasis cell line. It also modulated factors involved in the MAPK pathway and the cell cycle. In the psoriasis-like mice model, cold plasma resulted in improvements in skin thickness, erythema, scaling, and PASI. Additionally, decreases in inflammatory cytokines like INF-γ, IL-23, and S100a7 were observed, along with improvements in MAPK pathway activation, apoptosis, and other psoriasis-related factors.

Conclusion: Through in vitro and in vivo studies, our research highlights the potential of cold plasma as a novel therapeutic approach for psoriasis. Furthermore, cold plasma could serve as an adjunctive treatment for skin immunological diseases.

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来源期刊
Skin Research and Technology
Skin Research and Technology 医学-皮肤病学
CiteScore
3.30
自引率
9.10%
发文量
95
审稿时长
6-12 weeks
期刊介绍: Skin Research and Technology is a clinically-oriented journal on biophysical methods and imaging techniques and how they are used in dermatology, cosmetology and plastic surgery for noninvasive quantification of skin structure and functions. Papers are invited on the development and validation of methods and their application in the characterization of diseased, abnormal and normal skin. Topics include blood flow, colorimetry, thermography, evaporimetry, epidermal humidity, desquamation, profilometry, skin mechanics, epiluminiscence microscopy, high-frequency ultrasonography, confocal microscopy, digital imaging, image analysis and computerized evaluation and magnetic resonance. Noninvasive biochemical methods (such as lipids, keratin and tissue water) and the instrumental evaluation of cytological and histological samples are also covered. The journal has a wide scope and aims to link scientists, clinical researchers and technicians through original articles, communications, editorials and commentaries, letters, reviews, announcements and news. Contributions should be clear, experimentally sound and novel.
期刊最新文献
Using Wavelet Analysis of Blood Flow Oscillations to Investigate Differences in Skin Blood Flow Regulations Between the Upper and Lower Limbs. Cold Plasma Ameliorates Imiquimod-Induced Psoriasis-Like Skin Inflammation in Mice. Commentary on "Analysis of Factors Influencing the Recurrence of Diabetic Foot Ulcers". Development and Validation of a Total Body Photo-Numeric Freckling Density Scale. Involvement of Sex Hormones and Their Receptors in the Pathogenesis of Classic Kaposi's Sarcoma in Xinjiang.
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