{"title":"基于光声遥感显微技术的全纤维微型非接触式光声探针,用于体内血管成像。","authors":"Xingye Tang, Jiasheng Zhou, Siqi Liang, Jitong Zhang, Junjie Xiong, Lin Ma, Sung-Liang Chen","doi":"10.1364/OL.539208","DOIUrl":null,"url":null,"abstract":"<p><p>Photoacoustic (PA) remote sensing (PARS) microscopy represents a significant advancement by eliminating the need for traditional acoustic coupling media in PA microscopy (PAM), thereby broadening its potential applications. However, current PARS microscopy setups predominantly rely on free-space optical components, which can be cumbersome to implement and limit the scope of imaging applications. In this study, we develop an all-fiber miniature non-contact PA probe based on PARS microscopy, utilizing a 532-nm excitation wavelength, and showcase its effectiveness in in vivo vascular imaging. Our approach integrates various fiber-optic components, including a wavelength division multiplexer, a mode field adaptor, a fiber lens, and an optical circulator, to streamline the implementation of the PARS microscopy system. Additionally, we have successfully developed a miniature PA probe with a diameter of 4 mm. The efficacy of our imaging setup is demonstrated through in vivo imaging of mouse brain vessels. By introducing this all-fiber miniature PA probe, our work may open up new opportunities for non-contact PAM applications.</p>","PeriodicalId":19540,"journal":{"name":"Optics letters","volume":null,"pages":null},"PeriodicalIF":3.1000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"All-fiber miniature non-contact photoacoustic probe based on photoacoustic remote sensing microscopy for vascular imaging in vivo.\",\"authors\":\"Xingye Tang, Jiasheng Zhou, Siqi Liang, Jitong Zhang, Junjie Xiong, Lin Ma, Sung-Liang Chen\",\"doi\":\"10.1364/OL.539208\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Photoacoustic (PA) remote sensing (PARS) microscopy represents a significant advancement by eliminating the need for traditional acoustic coupling media in PA microscopy (PAM), thereby broadening its potential applications. However, current PARS microscopy setups predominantly rely on free-space optical components, which can be cumbersome to implement and limit the scope of imaging applications. In this study, we develop an all-fiber miniature non-contact PA probe based on PARS microscopy, utilizing a 532-nm excitation wavelength, and showcase its effectiveness in in vivo vascular imaging. Our approach integrates various fiber-optic components, including a wavelength division multiplexer, a mode field adaptor, a fiber lens, and an optical circulator, to streamline the implementation of the PARS microscopy system. Additionally, we have successfully developed a miniature PA probe with a diameter of 4 mm. The efficacy of our imaging setup is demonstrated through in vivo imaging of mouse brain vessels. By introducing this all-fiber miniature PA probe, our work may open up new opportunities for non-contact PAM applications.</p>\",\"PeriodicalId\":19540,\"journal\":{\"name\":\"Optics letters\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2024-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Optics letters\",\"FirstCategoryId\":\"101\",\"ListUrlMain\":\"https://doi.org/10.1364/OL.539208\",\"RegionNum\":2,\"RegionCategory\":\"物理与天体物理\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"OPTICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Optics letters","FirstCategoryId":"101","ListUrlMain":"https://doi.org/10.1364/OL.539208","RegionNum":2,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"OPTICS","Score":null,"Total":0}
引用次数: 0
摘要
光声(PA)遥感(PARS)显微镜无需使用传统的 PA 显微镜(PAM)中的声耦合介质,从而拓宽了其潜在应用领域,是一项重大进步。然而,目前的 PARS 显微镜装置主要依赖于自由空间光学元件,实施起来非常麻烦,而且限制了成像应用的范围。在本研究中,我们利用 532-nm 的激发波长,开发了一种基于 PARS 显微镜的全光纤微型非接触式 PA 探头,并展示了其在体内血管成像中的有效性。我们的方法集成了各种光纤组件,包括波分复用器、模式场适配器、光纤透镜和光循环器,以简化 PARS 显微系统的实施。此外,我们还成功开发了直径为 4 毫米的微型 PA 探头。通过对小鼠脑血管的活体成像,证明了我们成像装置的功效。通过引入这种全纤维微型 PA 探头,我们的工作可能会为非接触式 PAM 应用带来新的机遇。
All-fiber miniature non-contact photoacoustic probe based on photoacoustic remote sensing microscopy for vascular imaging in vivo.
Photoacoustic (PA) remote sensing (PARS) microscopy represents a significant advancement by eliminating the need for traditional acoustic coupling media in PA microscopy (PAM), thereby broadening its potential applications. However, current PARS microscopy setups predominantly rely on free-space optical components, which can be cumbersome to implement and limit the scope of imaging applications. In this study, we develop an all-fiber miniature non-contact PA probe based on PARS microscopy, utilizing a 532-nm excitation wavelength, and showcase its effectiveness in in vivo vascular imaging. Our approach integrates various fiber-optic components, including a wavelength division multiplexer, a mode field adaptor, a fiber lens, and an optical circulator, to streamline the implementation of the PARS microscopy system. Additionally, we have successfully developed a miniature PA probe with a diameter of 4 mm. The efficacy of our imaging setup is demonstrated through in vivo imaging of mouse brain vessels. By introducing this all-fiber miniature PA probe, our work may open up new opportunities for non-contact PAM applications.
期刊介绍:
The Optical Society (OSA) publishes high-quality, peer-reviewed articles in its portfolio of journals, which serve the full breadth of the optics and photonics community.
Optics Letters offers rapid dissemination of new results in all areas of optics with short, original, peer-reviewed communications. Optics Letters covers the latest research in optical science, including optical measurements, optical components and devices, atmospheric optics, biomedical optics, Fourier optics, integrated optics, optical processing, optoelectronics, lasers, nonlinear optics, optical storage and holography, optical coherence, polarization, quantum electronics, ultrafast optical phenomena, photonic crystals, and fiber optics. Criteria used in determining acceptability of contributions include newsworthiness to a substantial part of the optics community and the effect of rapid publication on the research of others. This journal, published twice each month, is where readers look for the latest discoveries in optics.