Xinyu Hu, Bob van Sluijs, Óscar García-Blay, Wilhelm T S Huck, Maike M K Hansen
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引用次数: 0
摘要
了解复杂生物系统中单个细胞的分子特征对于破译细胞异质性和揭示调控机制至关重要。在此，我们介绍一种利用空间单细胞图谱同时多重检测小鼠胚胎干细胞中选定的 mRNA 和（磷酸化）蛋白质的方案。我们介绍了使用单链 DNA（ssDNA）标记的抗生素、挂锁探针和滚动圈扩增来实现亚细胞分辨率下 mRNA 和（磷酸）蛋白同步可视化的步骤。该方案可用于识别异质生物微环境中的细胞。有关该方案使用和执行的完整细节，请参阅 Hu 等人的文章1。
Protocol for simultaneous detection of mRNAs and (phospho-)proteins with ARTseq-FISH in mouse embryonic stem cells.
Understanding the molecular signatures of individual cells within complex biological systems is crucial for deciphering cellular heterogeneity and uncovering regulatory mechanisms. Here, we present a protocol for simultaneous multiplexed detection of selected mRNAs and (phospho-)proteins in mouse embryonic stem cells using spatial single-cell profiling. We describe steps for employing single-stranded DNA (ssDNA)-labeled antibo'dies, padlock probes, and rolling circle amplification to achieve simultaneous visualization of mRNAs and (phospho-)proteins at subcellular resolution. This protocol has potential application in identifying cells in heterogeneous biological microenvironments. For complete details on the use and execution of this protocol, please refer to Hu et al.1.
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