Mey-Fann Lee, Yi-Hsing Chen, Chu-Hui Chiang, Chi-Sheng Wu, Min-Hou Li, Nancy M Wang
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引用次数: 0
摘要
目的:特应性行军是指在儿童早期出现特应性皮炎。最近,我们通过对来自屋尘螨和蟑螂的空气过敏原进行皮肤过敏,建立了特应性行军小鼠模型。本研究旨在利用这一模型,评估携带特异性抗原的乳酸乳球菌口服免疫疗法对特应性进行性皮炎进展的疗效:方法:尘螨主要过敏原Der p 2和蟑螂Per a 2-372在乳球菌中表达为融合重组克隆(D2P2)。将 L. lactis-D2P2 灌胃给航空过敏原贴片致敏小鼠,每天一次,共 35 次。对血清中的免疫变量、搔抓行为、气道高反应性(AHR)以及肺部和皮肤的病理变化进行了评估:结果:我们的数据显示,与对照组小鼠相比,L.lactis-D2P2能显著降低免疫球蛋白E的总水平,减少抓挠行为,缓解气道高反应性。皮肤和肺组织的组织学分析表明,L.lactis-D2P2 通过减少嗜酸性粒细胞浸润和降低关键细胞因子白细胞介素(IL)-31 和 IL-13 的表达来调节免疫反应:结论:研究结果表明,与传统的皮下过敏原特异性免疫疗法相比,L. lactis-D2P2 的粘膜过敏原特异性免疫疗法是一种更具成本效益的替代疗法。这项研究为开发新型口服蛋白疫苗以早期预防过敏症提供了一个前景广阔的平台。
Oral Administration of Lactococcus lactis Expressing Mite and Cockroach Major Allergens Alleviates Progression of Atopic March in a Mouse Model.
Purpose: Atopic march is defined as the development of atopic dermatitis in early childhood. We recently developed an atopic march mouse model through skin sensitization with aeroallergens from house dust mites and cockroaches. Using this model, this study aimed to evaluate the oral immunotherapy efficacy of Lactococcus lactis harboring specific antigens on the progression of atopic march.
Methods: Dust mite major allergen Der p 2 and cockroach Per a 2-372 were expressed in L. lactis as a fusion recombinant clone (D2P2). L. lactis-D2P2 was administered intragastrically to Aeroallergen patch-sensitized mice once a day for a total of 35 times. The immunological variables in sera, scratching behavior, airway hyperresponsiveness (AHR), and pathology of lungs and skin were evaluated.
Results: Our data showed that L. lactis-D2P2 significantly lowered total immunoglobulin E levels, decreased scratch bouts, and relieved AHR compared with the control mice. Histological analysis of the skin and lung tissue demonstrated the therapeutic effects of L. lactis-D2P2 to modulate immune responses via decreased eosinophil infiltration and reduced expression of key cytokines, interleukin (IL)-31 and IL-13, respectively.
Conclusions: The results imply that mucosal allergen-specific immunotherapy of L. lactis-D2P2 is a more cost-effective alternative to conventional subcutaneous allergen-specific immunotherapy. This study provides a promising platform for the development of novel oral protein-based vaccines in the early prevention of allergies.
期刊介绍:
The journal features cutting-edge original research, brief communications, and state-of-the-art reviews in the specialties of allergy, asthma, and immunology, including clinical and experimental studies and instructive case reports. Contemporary reviews summarize information on topics for researchers and physicians in the fields of allergy and immunology. As of January 2017, AAIR do not accept case reports. However, if it is a clinically important case, authors can submit it in the form of letter to the Editor. Editorials and letters to the Editor explore controversial issues and encourage further discussion among physicians dealing with allergy, immunology, pediatric respirology, and related medical fields. AAIR also features topics in practice and management and recent advances in equipment and techniques for clinicians concerned with clinical manifestations of allergies and pediatric respiratory diseases.