Nan Wang, Qianqian Ma, Junxian Zhang, Jie Wang, Xiaojun Li, Yan Liang, Xueqiong Wu
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Finally, GEO databases and RT-qPCR were used to verify the results of expression profile.</p><p><strong>Result: </strong>After MTB infection, most upregulated DE genes in mice were immune-related genes, including <i>cxcl9</i>, <i>camp</i>, <i>cfb</i>, <i>c4b</i>, <i>serpina3g</i>, and <i>ngp</i>. Downregulated DE genes included <i>lrrc74b</i>, <i>sult1d1</i>, <i>cxxc4</i>, and <i>grip2</i>. After treatment with NBXH, especially high-dose NBXH, the abnormal gene expression was significantly corrected. Some DE genes have been confirmed in multiple GEO datasets or in pulmonary TB patients through RT-qPCR.</p><p><strong>Conclusion: </strong>MTB infection led to extensive changes in host gene expression and mainly caused the host's anti-TB immune responses. The treatment using high-dose NBXH partially repaired the abnormal gene expression, further enhanced the anti-TB immunity included autophagy and NK cell-mediated cytotoxicity, and had a certain inhibitory effect on overactivated immune responses.</p>","PeriodicalId":12491,"journal":{"name":"Frontiers in Pharmacology","volume":null,"pages":null},"PeriodicalIF":4.4000,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11446850/pdf/","citationCount":"0","resultStr":"{\"title\":\"Transcriptomics-based anti-tuberculous mechanism of traditional Chinese polyherbal preparation NiuBeiXiaoHe intermediates.\",\"authors\":\"Nan Wang, Qianqian Ma, Junxian Zhang, Jie Wang, Xiaojun Li, Yan Liang, Xueqiong Wu\",\"doi\":\"10.3389/fphar.2024.1415951\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Integrated traditional Chinese medicine and biomedicine is an effective method to treat tuberculosis (TB). 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引用次数: 0
摘要
背景:中药与生物医药相结合是治疗结核病的有效方法。在我们之前的研究中,中药制剂牛膝细辛(NBXH)在动物实验和临床实践中取得了明显的抗结核效果。然而,NBXH 的作用机制尚未阐明:方法:采集外周血单核细胞(PBMC)提取 mRNA,利用基因芯片技术获得差异表达基因(DE)。最后,利用 GEO 数据库和 RT-qPCR 验证表达谱结果:结果:小鼠感染MTB后,大多数上调的DE基因为免疫相关基因,包括cxcl9、camp、cfb、c4b、serpina3g和ngp。下调的 DE 基因包括 lrrc74b、sult1d1、cxxc4 和 grip2。经 NBXH(尤其是大剂量 NBXH)治疗后,异常基因表达得到明显纠正。一些 DE 基因已在多个 GEO 数据集或肺结核患者中通过 RT-qPCR 得到证实:结论:MTB 感染导致宿主基因表达的广泛变化,主要引起宿主的抗结核免疫反应。结论:MTB 感染导致宿主基因表达发生广泛变化,主要引起宿主的抗结核免疫反应,大剂量 NBXH 治疗可部分修复异常基因表达,进一步增强自噬和 NK 细胞介导的细胞毒性等抗结核免疫能力,并对过度激活的免疫反应有一定的抑制作用。
Transcriptomics-based anti-tuberculous mechanism of traditional Chinese polyherbal preparation NiuBeiXiaoHe intermediates.
Background: Integrated traditional Chinese medicine and biomedicine is an effective method to treat tuberculosis (TB). In our previous research, traditional Chinese medicine preparation NiuBeiXiaoHe (NBXH) achieved obvious anti-TB effects in animal experiments and clinical practice. However, the action mechanism of NBXH has not been elucidated.
Method: Peripheral blood mononuclear cells (PBMCs) were collected to extract mRNA and differentially expressed (DE) genes were obtained using gene microarray technology. Finally, GEO databases and RT-qPCR were used to verify the results of expression profile.
Result: After MTB infection, most upregulated DE genes in mice were immune-related genes, including cxcl9, camp, cfb, c4b, serpina3g, and ngp. Downregulated DE genes included lrrc74b, sult1d1, cxxc4, and grip2. After treatment with NBXH, especially high-dose NBXH, the abnormal gene expression was significantly corrected. Some DE genes have been confirmed in multiple GEO datasets or in pulmonary TB patients through RT-qPCR.
Conclusion: MTB infection led to extensive changes in host gene expression and mainly caused the host's anti-TB immune responses. The treatment using high-dose NBXH partially repaired the abnormal gene expression, further enhanced the anti-TB immunity included autophagy and NK cell-mediated cytotoxicity, and had a certain inhibitory effect on overactivated immune responses.
期刊介绍:
Frontiers in Pharmacology is a leading journal in its field, publishing rigorously peer-reviewed research across disciplines, including basic and clinical pharmacology, medicinal chemistry, pharmacy and toxicology. Field Chief Editor Heike Wulff at UC Davis is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.