蛋白酶体抑制剂可抑制小鼠全身性脂多糖挑战时脂钙蛋白-2的诱导。

IF 3.3 3区 医学 Q2 NEUROSCIENCES Molecular Brain Pub Date : 2024-10-03 DOI:10.1186/s13041-024-01147-w
Jin-Sil Bae, Ji-Eun Heo, Kwon-Yul Ryu
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引用次数: 0

摘要

脂联素-2(Lcn2)是一种由免疫激活细胞(包括反应性星形胶质细胞)分泌的蛋白质,对大脑有害并诱发神经退行性变。我们以前曾发现,蛋白酶体抑制剂硼替佐米(BTZ)治疗后,原代小鼠星形胶质细胞中的 Lcn2 水平会降低。然而,在体内是否也能观察到硼替佐米(BTZ)治疗后 Lcn2 水平的降低,以及在脂多糖(LPS)诱导的体内全身性炎症过程中,Lcn2 是否会降低神经毒性,这些仍是未知数。为了回答这些问题,我们对小鼠进行了腹腔注射 LPS 挑战实验,发现 Lcn2 在大脑中的水平显著升高,再现了使用星形胶质细胞进行的体外实验。与 LPS 处理的对照组相比,联合给药 LPS 和 BTZ 可降低 Lcn2 的水平。在 LPS 挑战下,小鼠大脑中胶质标记基因的表达水平上调。值得注意的是,联合使用 BTZ 会阻碍这种上调。综上所述,我们的研究结果表明,BTZ 可降低 LPS 诱导的 Lcn2 水平,并可减轻 LPS 诱导的小鼠神经炎症和神经毒性。
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Proteasome inhibition suppresses the induction of lipocalin-2 upon systemic lipopolysaccharide challenge in mice.

Lipocalin-2 (Lcn2), a protein secreted by immune-activated cells, including reactive astrocytes, is detrimental to the brain and induces neurodegeneration. We previously showed that Lcn2 levels are reduced in primary mouse astrocytes after treatment with the proteasome inhibitor bortezomib (BTZ). However, it remains unknown whether a decrease in Lcn2 levels after BTZ treatment can also be observed in vivo and whether it reduces neurotoxicity during lipopolysaccharide (LPS)-induced systemic inflammation in vivo. To answer these questions, we performed LPS challenge experiments by intraperitoneal injection in mice and found that Lcn2 levels were significantly increased in the brain, recapitulating in vitro experiments using astrocytes. Co-administration of LPS and BTZ reduced the Lcn2 levels compared to the levels in LPS-treated controls. Upon LPS challenge, the expression levels of glial marker genes were upregulated in the mouse brain. Of note, this upregulation was hampered by the co-administration of BTZ. Taken together, our results suggested that BTZ can reduce LPS-induced Lcn2 levels and may alleviate LPS-induced neuroinflammation and neurotoxicity in mice.

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来源期刊
Molecular Brain
Molecular Brain NEUROSCIENCES-
CiteScore
7.30
自引率
0.00%
发文量
97
审稿时长
>12 weeks
期刊介绍: Molecular Brain is an open access, peer-reviewed journal that considers manuscripts on all aspects of studies on the nervous system at the molecular, cellular, and systems level providing a forum for scientists to communicate their findings. Molecular brain research is a rapidly expanding research field in which integrative approaches at the genetic, molecular, cellular and synaptic levels yield key information about the physiological and pathological brain. These studies involve the use of a wide range of modern techniques in molecular biology, genomics, proteomics, imaging and electrophysiology.
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