Suzanne Schmidt, Robert Murphy, Joel Vizueta, Signe Kjærsgaard Schierbech, Benjamin H. Conlon, Nina B. Kreuzenbeck, Sabine M. E. Vreeburg, Lennart J. J. van de Peppel, Duur K. Aanen, Kolotchèlèma S. Silué, N’Golo A. Kone, Christine Beemelmanns, Tilmann Weber, Michael Poulsen
{"title":"比较基因组学揭示了白蚁养殖的真菌物种(白蚁真菌)中具有不同进化轨迹的丰富的生物合成基因簇","authors":"Suzanne Schmidt, Robert Murphy, Joel Vizueta, Signe Kjærsgaard Schierbech, Benjamin H. Conlon, Nina B. Kreuzenbeck, Sabine M. E. Vreeburg, Lennart J. J. van de Peppel, Duur K. Aanen, Kolotchèlèma S. Silué, N’Golo A. Kone, Christine Beemelmanns, Tilmann Weber, Michael Poulsen","doi":"10.1038/s42003-024-06887-y","DOIUrl":null,"url":null,"abstract":"The use of compounds produced by hosts or symbionts for defence against antagonists has been identified in many organisms, including in fungus-farming termites (Macrotermitinae). The obligate mutualistic fungus Termitomyces plays a pivotal role in plant biomass decomposition and as the primary food source for these termites. Despite the isolation of various specialized metabolites from different Termitomyces species, our grasp of their natural product repertoire remains incomplete. To address this knowledge gap, we conducted a comprehensive analysis of 39 Termitomyces genomes, representing 21 species associated with members of five termite host genera. We identified 754 biosynthetic gene clusters (BGCs) coding for specialized metabolites and categorized 660 BGCs into 61 biosynthetic gene cluster families (GCFs) spanning five compound classes. Seven GCFs were shared by all 21 Termitomyces species and 21 GCFs were present in all genomes of subsets of species. Evolutionary constraint analyses on the 25 most abundant GCFs revealed distinctive evolutionary histories, signifying that millions of years of termite-fungus symbiosis have influenced diverse biosynthetic pathways. This study unveils a wealth of non-random and largely undiscovered chemical potential within Termitomyces and contributes to our understanding of the intricate evolutionary trajectories of biosynthetic gene clusters in the context of long-standing symbiosis. Systematic identification and comparison of biosynthetic gene clusters among Termitomyces species provides insight into their evolutionary history and potential functions of BGCs and the chemicals synthesized by these pathways.","PeriodicalId":10552,"journal":{"name":"Communications Biology","volume":null,"pages":null},"PeriodicalIF":5.2000,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s42003-024-06887-y.pdf","citationCount":"0","resultStr":"{\"title\":\"Comparative genomics unravels a rich set of biosynthetic gene clusters with distinct evolutionary trajectories across fungal species (Termitomyces) farmed by termites\",\"authors\":\"Suzanne Schmidt, Robert Murphy, Joel Vizueta, Signe Kjærsgaard Schierbech, Benjamin H. Conlon, Nina B. Kreuzenbeck, Sabine M. E. Vreeburg, Lennart J. J. van de Peppel, Duur K. Aanen, Kolotchèlèma S. Silué, N’Golo A. Kone, Christine Beemelmanns, Tilmann Weber, Michael Poulsen\",\"doi\":\"10.1038/s42003-024-06887-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The use of compounds produced by hosts or symbionts for defence against antagonists has been identified in many organisms, including in fungus-farming termites (Macrotermitinae). The obligate mutualistic fungus Termitomyces plays a pivotal role in plant biomass decomposition and as the primary food source for these termites. Despite the isolation of various specialized metabolites from different Termitomyces species, our grasp of their natural product repertoire remains incomplete. To address this knowledge gap, we conducted a comprehensive analysis of 39 Termitomyces genomes, representing 21 species associated with members of five termite host genera. We identified 754 biosynthetic gene clusters (BGCs) coding for specialized metabolites and categorized 660 BGCs into 61 biosynthetic gene cluster families (GCFs) spanning five compound classes. Seven GCFs were shared by all 21 Termitomyces species and 21 GCFs were present in all genomes of subsets of species. Evolutionary constraint analyses on the 25 most abundant GCFs revealed distinctive evolutionary histories, signifying that millions of years of termite-fungus symbiosis have influenced diverse biosynthetic pathways. This study unveils a wealth of non-random and largely undiscovered chemical potential within Termitomyces and contributes to our understanding of the intricate evolutionary trajectories of biosynthetic gene clusters in the context of long-standing symbiosis. 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Comparative genomics unravels a rich set of biosynthetic gene clusters with distinct evolutionary trajectories across fungal species (Termitomyces) farmed by termites
The use of compounds produced by hosts or symbionts for defence against antagonists has been identified in many organisms, including in fungus-farming termites (Macrotermitinae). The obligate mutualistic fungus Termitomyces plays a pivotal role in plant biomass decomposition and as the primary food source for these termites. Despite the isolation of various specialized metabolites from different Termitomyces species, our grasp of their natural product repertoire remains incomplete. To address this knowledge gap, we conducted a comprehensive analysis of 39 Termitomyces genomes, representing 21 species associated with members of five termite host genera. We identified 754 biosynthetic gene clusters (BGCs) coding for specialized metabolites and categorized 660 BGCs into 61 biosynthetic gene cluster families (GCFs) spanning five compound classes. Seven GCFs were shared by all 21 Termitomyces species and 21 GCFs were present in all genomes of subsets of species. Evolutionary constraint analyses on the 25 most abundant GCFs revealed distinctive evolutionary histories, signifying that millions of years of termite-fungus symbiosis have influenced diverse biosynthetic pathways. This study unveils a wealth of non-random and largely undiscovered chemical potential within Termitomyces and contributes to our understanding of the intricate evolutionary trajectories of biosynthetic gene clusters in the context of long-standing symbiosis. Systematic identification and comparison of biosynthetic gene clusters among Termitomyces species provides insight into their evolutionary history and potential functions of BGCs and the chemicals synthesized by these pathways.
期刊介绍:
Communications Biology is an open access journal from Nature Research publishing high-quality research, reviews and commentary in all areas of the biological sciences. Research papers published by the journal represent significant advances bringing new biological insight to a specialized area of research.