高级糖化终产物通过 PKC/p47 phox 轴促进骨骼肌细胞产生 ROS。

IF 2.6 4区医学 Q2 PHYSIOLOGY Journal of Physiological Sciences Pub Date : 2024-10-05 DOI:10.1186/s12576-024-00944-1

Shinichiro Suzuki, Tatsuya Hayashi, Tatsuro Egawa

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引用次数: 0

摘要

高级糖化终产物（AGEs）是包括肌肉疏松症在内的多种疾病的危险因素。AGEs 的有害影响之一是诱导骨骼肌产生异常的活性氧（ROS）。然而，人们对其潜在机制仍知之甚少。因此，本研究旨在阐明 AGEs 如何诱导骨骼肌细胞产生 ROS。本研究表明，AGEs 处理可促进肌母细胞和肌管中 ROS 的产生，而 PKC 抑制剂可抑制 AGEs 刺激下的 ROS 产生。p47 phox 被 PKCα 等激酶磷酸化是形成 Nox2 复合物的必要条件，而 Nox2 复合物会诱导 ROS 的产生。在本研究中，AGEs 处理促进了成肌细胞和肌管中 PKCα 和 p47 phox 的磷酸化。我们的研究结果表明，AGEs 可通过磷酸化骨骼肌细胞中的 PKCα 和 p47 phox 促进 ROS 的产生。

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Advanced glycation end products promote ROS production via PKC/p47 phox axis in skeletal muscle cells.

Advanced glycation end products (AGEs) are risk factors for various diseases, including sarcopenia. One of the deleterious effects of AGEs is the induction of abnormal reactive oxygen species (ROS) production in skeletal muscle. However, the underlying mechanism remains poorly understood. Therefore, the aim of this study was to elucidate how AGEs induce ROS production in skeletal muscle cells. This study demonstrated that AGEs treatment promoted ROS production in myoblasts and myotubes while PKC inhibitor abolished ROS production by AGEs stimulation. Phosphorylation of p47 phox by kinases such as PKCα is required to form the Nox2 complex, which induces ROS production. In this study, AGEs treatment promoted the phosphorylation of PKCα and p47 phox in myoblasts and myotubes. Our findings suggest that AGEs promote ROS production through the phosphorylation of PKCα and p47 phox in skeletal muscle cells.

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来源期刊

Journal of Physiological Sciences 医学-生理学

CiteScore

4.40

自引率

4.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal of Physiological Sciences publishes peer-reviewed original papers, reviews, short communications, technical notes, and letters to the editor, based on the principles and theories of modern physiology and addressed to the international scientific community. All fields of physiology are covered, encompassing molecular, cellular and systems physiology. The emphasis is on human and vertebrate physiology, but comparative papers are also considered. The process of obtaining results must be ethically sound. Fields covered: Adaptation and environment Autonomic nervous function Biophysics Cell sensors and signaling Central nervous system and brain sciences Endocrinology and metabolism Excitable membranes and neural cell physiology Exercise physiology Gastrointestinal and kidney physiology Heart and circulatory physiology Molecular and cellular physiology Muscle physiology Physiome/systems biology Respiration physiology Senses.