三阴性乳腺癌患者血清和粪便代谢物谱与肠道微生物组的关系

IF 1.8 Q3 ONCOLOGY Breast Cancer : Basic and Clinical Research Pub Date : 2024-10-05 eCollection Date: 2024-01-01 DOI:10.1177/11782234241285645
Jiawei Liu, Jing Shi, Tingting Zhang, Mie Chen, Zhennan Li, Cheng Lu, Fengliang Wang
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引用次数: 0

摘要

背景:三阴性乳腺癌(TNBC)是乳腺癌的一种亚型,由于缺乏有效的靶向疗法,其预后较差。新的证据表明,肠道微生物群及其代谢产物在 TNBC 的发生和发展中起着关键作用。本研究旨在探讨与 TNBC 相关的代谢变化和潜在机制:本研究旨在探讨 TNBC 靶向代谢物与肠道微生物群之间的潜在关系:我们招募了8名参与者,包括4名TNBC患者和4名良性纤维腺瘤对照组:方法:使用元基因组学分析粪便样本中的肠道微生物群。采用液相色谱-质谱法(LC-MS)鉴定血清和粪便样本中的不同代谢物。利用斯皮尔曼相关分析法分析了肠道微生物群与代谢物之间的相关性:结果:对 TNBC 组血清代谢物变化的分析表明,羧酸及其衍生物、苯和取代衍生物的变化尤为明显。京都基因与基因组百科全书(KEGG)代谢途径分析显示,有18条途径发生了显著富集。在粪便代谢物方面,两组之间的差异还包括羧酸及其衍生物、苯和取代衍生物,根据 KEGG 通路分析,28 条代谢通路被富集。元基因组学分析表明,Anaerococcus、Fischerella 和 Schizosaccharomyces 的相对丰度在属种水平上存在差异,而这些属种以前曾与乳腺癌有关。此外,4种血清代谢物-谷氨酰胺、柠檬酸盐、肌酐和肌酸,以及9种粪便代谢物与上述微生物群相关:我们的研究结果突显了 TNBC 患者血清和粪便中不同的代谢物特征。肠道微生物群及其相关代谢物的鉴定为了解 TNBC 的病理生理机制提供了新的视角。
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Serum and Fecal Metabolite Profiles Linking With Gut Microbiome in Triple-Negative Breast Cancer Patients.

Background: Triple-negative breast cancer (TNBC) is a subtype of breast cancer characterized by poor prognosis due to the absence of effective targeted therapies. Emerging evidence indicates that the gut microbiota and its metabolites play a key role in the occurrence and development of TNBC. This study aimed to explore the metabolic changes and potential mechanisms associated with TNBC.

Objectives: This study aimed to explore the potential relationship between targeted metabolites and the gut microbiota in TNBC.

Design: We recruited 8 participants, including 4 with TNBC and 4 with benign fibroadenomas as controls.

Methods: The gut microbiota was analyzed using metagenomics on fecal samples. Liquid chromatography-mass spectrometry (LC-MS) was employed to identify differential metabolites in serum and fecal samples. The correlation between the gut microbiota and metabolites was analyzed using Spearman's correlation analysis.

Results: Analysis of altered serum metabolites in the TNBC group revealed changes, particularly in carboxylic acids and derivatives, benzene, and substituted derivatives. Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway analysis revealed significant enrichment in 18 pathways. Regarding fecal metabolites, differences between the 2 groups also included carboxylic acids and derivatives, benzene, and substituted derivatives, with 28 metabolic pathways enriched based on KEGG pathway analysis. Metagenomics analysis showed differences in the relative abundance of Anaerococcus, Fischerella, and Schizosaccharomyces at the genus level, which have been previously associated with breast cancer. Furthermore, 4 serum metabolites-L-glutamine, citrate, creatinine, and creatine-along with 9 fecal metabolites, were associated with the aforementioned microbiota.

Conclusion: Our findings highlight distinct metabolite profiles in the serum and feces of patients with TNBC. The identification of gut microbiota and their associated metabolites provides new insights into the pathophysiological mechanisms underlying TNBC.

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来源期刊
CiteScore
5.10
自引率
3.40%
发文量
22
审稿时长
8 weeks
期刊介绍: Breast Cancer: Basic and Clinical Research is an international, open access, peer-reviewed, journal which considers manuscripts on all areas of breast cancer research and treatment. We welcome original research, short notes, case studies and review articles related to breast cancer-related research. Specific areas of interest include, but are not limited to, breast cancer sub types, pathobiology, metastasis, genetics and epigenetics, mammary gland biology, breast cancer models, prevention, detection, therapy and clinical interventions, and epidemiology and population genetics.
期刊最新文献
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