术前应用糖皮质激素对伴有脉络膜脱离的流变性视网膜脱离患者玻璃体副视网膜相关蛋白表达的影响

IF 1.7 4区医学 Q3 OPHTHALMOLOGY Current Eye Research Pub Date : 2024-10-07 DOI:10.1080/02713683.2024.2408755

Yue Guo, Ziyang Ye, Can Deng, Lin Wang, Qihong Gu, Kangkang Ji, Xiaomeng Li, Kai Dong

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引用次数: 0

摘要

目的凋亡诱导因子（AIF）、巨噬细胞迁移抑制因子（MIF）、白细胞介素-1β（IL-1β）、聚ADP核糖聚合酶-1（PARP-1）、聚（ADP-核糖）（PAR）、α-突触核蛋白（α-SYN）、单核细胞趋化蛋白-1（MCP-1）和肿瘤坏死因子-α（TNF-α）的蛋白浓度、单核细胞趋化蛋白-1（MCP-1）和肿瘤坏死因子-α（TNF-α）。研究方法共纳入 57 例患者样本。30 例 RRD 患者为对照组，27 例 RRDCD 患者为实验组（16 例术前使用糖皮质激素（GC+），11 例术前未使用糖皮质激素（GC-））。采用酶联免疫吸附试验（ELISA）检测对照组和实验组患者玻璃体内 AIF、MIF、IL-1β、PARP-1、PAR、α-SYN、MCP-1 和 TNF-α 的水平：RRD组玻璃体内AIF浓度（9.96 ± 2.78 ng/ml）高于RRDCD（GC+）组（7.65 ± 2.13 ng/ml，P = 0.006），RRDCD（GC+）组低于RRDCD（GC-）组（10.28 ± 2.81 ng/ml）（P = 0.013）。玻璃体液中 MIF 的浓度在 RRDCD（GC+）组（61.21 ± 17.56 ng/ml）低于 RRDCD（GC-）组（74.30 ± 9.66 ng/ml，p = 0.039）。实验组中，术前PVR分级C级（284.93 ± 54.96 ng/ml）组的MCP-1蛋白浓度高于D级（225.94 ± 24.05 ng/ml）（P = 0.050）；RRDCD（GC+）组患者眼轴的MIF蛋白浓度低于P = 0.043）.结论：结论：Parthanatos相关蛋白在RRDCD（GC+）患者玻璃体内表达较低，术前使用糖皮质激素治疗可降低Parthanatos相关蛋白的表达。

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Effect of Preoperative Glucocorticoid Application on Vitreous Parthanatos-Related Protein Expression in Patients with Rhegmatogenous Retinal Detachment Associated with Choroidal Detachment.

Purpose: The protein concentrations of apoptosis inducing factor (AIF), macrophage migration inhibitory factor (MIF), interleukin-1β (IL-1β), poly ADP ribose polymerase-1 (PARP-1), poly (ADP-ribose) (PAR), α-synuclein (α-SYN), monocyte chemotactic protein‑1 (MCP-1) and tumor necrosis factor-α (TNF-α) in the vitreous of eyes with rhegmatogenous retinal detachment associated with choroidal detachment (RRDCD) were observed and analyzed.

Methods: A total of 57 patients' samples were included. 30 patients with RRD were set as the control group, 27 patients with RRDCD were set as the experimental group (16 patients with preoperative glucocorticosteroid (GC+) and 11 patients without preoperative glucocorticosteroid (GC-)). The levels of AIF, MIF, IL-1β, PARP-1, PAR, α-SYN, MCP-1 and TNF-α in vitreous of patients in the control and experimental groups were detected by enzyme-linked immunosorbent assay (ELISA).

Results: The concentration of AIF in the vitreous was higher in the RRD group (9.96 ± 2.78 ng/ml) than in the RRDCD (GC+) group (7.65 ± 2.13 ng/ml, p = 0.006),the RRDCD (GC+) group was lower than the RRDCD (GC-) group (10.28 ± 2.81 ng/ml) (p = 0.013). The concentration of MIF in vitreous fluid was lower in the RRDCD (GC+) group (61.21 ± 17.56 ng/ml) than in the RRDCD (GC-) group (74.30 ± 9.66 ng/ml, p = 0.039). In the experimental group, the protein concentration of MCP-1 in the RRDCD (GC+) group was higher in the preoperative PVR grading C (284.93 ± 54.96 ng/ml) grade than in the D grade (225.94 ± 24.05 ng/ml) (p = 0.050); The protein concentration of MIF was lower in the RRDCD (GC+) group of patients with an ocular axis of <26 mm (56.19 ± 6.99 ng/ml) than in those with an ocular axis of ≥26 mm (76.26 ± 26.60 ng/ml, p = 0.043).

Conclusion: Low expression of Parthanatos-related proteins is present in the vitreous of patients with RRDCD (GC+), and preoperative treatment with glucocorticoids may reduce the expression of Parthanatos-related proteins.

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来源期刊

Current Eye Research 医学-眼科学

CiteScore

4.60

自引率

0.00%

发文量

163

审稿时长

12 months

期刊介绍： The principal aim of Current Eye Research is to provide rapid publication of full papers, short communications and mini-reviews, all high quality. Current Eye Research publishes articles encompassing all the areas of eye research. Subject areas include the following: clinical research, anatomy, physiology, biophysics, biochemistry, pharmacology, developmental biology, microbiology and immunology.