{"title":"通过单细胞基因组分析，利用四个细胞从混合血斑中识别个体。","authors":"Shunsuke Yamada, Yukiko Dozen, Kensho Tatsumi, Masaomi Okamoto, Chihiro Ikemoto, Tomomi Michiue, Atsushi Nagai","doi":"10.1016/j.forsciint.2024.112242","DOIUrl":null,"url":null,"abstract":"<p><p>We endeavored to identify individuals from mixed-blood spots by conducting a singlecell genomic analysis on four cells. Based on the electropherograms of 15 STRs (short tandem repeats) and amelogenin, we selected samples presumed to be nonmixed. These samples were then segregated into two groups and combined within eachgroup. We forecasted the DNA profiles of each donor using these predictions. The outcomes aligned with the original DNA profiles of each donor.</p>","PeriodicalId":12341,"journal":{"name":"Forensic science international","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Individual identification from mixed-blood spots by using four cells with single-cell genomic analysis.\",\"authors\":\"Shunsuke Yamada, Yukiko Dozen, Kensho Tatsumi, Masaomi Okamoto, Chihiro Ikemoto, Tomomi Michiue, Atsushi Nagai\",\"doi\":\"10.1016/j.forsciint.2024.112242\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>We endeavored to identify individuals from mixed-blood spots by conducting a singlecell genomic analysis on four cells. Based on the electropherograms of 15 STRs (short tandem repeats) and amelogenin, we selected samples presumed to be nonmixed. These samples were then segregated into two groups and combined within eachgroup. We forecasted the DNA profiles of each donor using these predictions. The outcomes aligned with the original DNA profiles of each donor.</p>\",\"PeriodicalId\":12341,\"journal\":{\"name\":\"Forensic science international\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-10-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Forensic science international\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.forsciint.2024.112242\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MEDICINE, LEGAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Forensic science international","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.forsciint.2024.112242","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MEDICINE, LEGAL","Score":null,"Total":0}
引用次数: 0
摘要
我们通过对四个细胞进行单细胞基因组分析，努力从混血样本中识别个体。根据 15 个 STR（短串联重复序列）和淀粉样蛋白的电泳图，我们选出了推测为非混血的样本。然后将这些样本分成两组，并在每组中进行组合。我们利用这些预测结果预测了每个捐献者的 DNA 图谱。结果与每个捐献者的原始 DNA 图谱一致。
Individual identification from mixed-blood spots by using four cells with single-cell genomic analysis.
We endeavored to identify individuals from mixed-blood spots by conducting a singlecell genomic analysis on four cells. Based on the electropherograms of 15 STRs (short tandem repeats) and amelogenin, we selected samples presumed to be nonmixed. These samples were then segregated into two groups and combined within eachgroup. We forecasted the DNA profiles of each donor using these predictions. The outcomes aligned with the original DNA profiles of each donor.
期刊介绍：
Forensic Science International is the flagship journal in the prestigious Forensic Science International family, publishing the most innovative, cutting-edge, and influential contributions across the forensic sciences. Fields include: forensic pathology and histochemistry, chemistry, biochemistry and toxicology, biology, serology, odontology, psychiatry, anthropology, digital forensics, the physical sciences, firearms, and document examination, as well as investigations of value to public health in its broadest sense, and the important marginal area where science and medicine interact with the law.
The journal publishes:
Case Reports
Commentaries
Letters to the Editor
Original Research Papers (Regular Papers)
Rapid Communications
Review Articles
Technical Notes.
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