{"title":"从毛细管电泳用熔融石英毛细管上去除聚酰亚胺涂层的简单方法。","authors":"Pan Wang, Yulin Cheng, Wei Wei","doi":"10.1016/j.chroma.2024.465395","DOIUrl":null,"url":null,"abstract":"<div><div>A simple and cost-effective method for making capillary electrophoresis (CE) detection windows is reported. A low-cost laser engraving machine equipped with a 450 nm high-power blue laser was used to remove the polyimide coating from the fused silica capillary by laser scanning the desired section. The entire process is automated and controlled in real time by software. This method can be easily adopted by many CE users for routine practice and is scalable for high-throughput production of detection windows across multiple capillaries.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1736 ","pages":"Article 465395"},"PeriodicalIF":3.8000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A simple method to remove polyimide coating from fused silica capillaries for capillary electrophoresis\",\"authors\":\"Pan Wang, Yulin Cheng, Wei Wei\",\"doi\":\"10.1016/j.chroma.2024.465395\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>A simple and cost-effective method for making capillary electrophoresis (CE) detection windows is reported. A low-cost laser engraving machine equipped with a 450 nm high-power blue laser was used to remove the polyimide coating from the fused silica capillary by laser scanning the desired section. The entire process is automated and controlled in real time by software. This method can be easily adopted by many CE users for routine practice and is scalable for high-throughput production of detection windows across multiple capillaries.</div></div>\",\"PeriodicalId\":347,\"journal\":{\"name\":\"Journal of Chromatography A\",\"volume\":\"1736 \",\"pages\":\"Article 465395\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2024-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Chromatography A\",\"FirstCategoryId\":\"1\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0021967324007696\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Chromatography A","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0021967324007696","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
摘要
本报告介绍了一种制作毛细管电泳（CE）检测窗的简单而经济的方法。使用配备 450 nm 高功率蓝色激光的低成本激光雕刻机，通过激光扫描所需的部分，去除熔融石英毛细管上的聚酰亚胺涂层。整个过程由软件自动实时控制。这种方法很容易被许多 CE 用户用于日常实践，并可扩展用于多个毛细管检测窗口的高通量生产。
A simple method to remove polyimide coating from fused silica capillaries for capillary electrophoresis
A simple and cost-effective method for making capillary electrophoresis (CE) detection windows is reported. A low-cost laser engraving machine equipped with a 450 nm high-power blue laser was used to remove the polyimide coating from the fused silica capillary by laser scanning the desired section. The entire process is automated and controlled in real time by software. This method can be easily adopted by many CE users for routine practice and is scalable for high-throughput production of detection windows across multiple capillaries.
期刊介绍：
The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.
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