John Roberts Padde , Yinghui Zhou , Yunxuan Chen , Yuxiao Zhu , Yuxuan Yang , Min Hou , Lu Chen , Zhipeng Xu , Donghui Zhang , Lin Chen , MinJun Ji
{"title":"库蚊对极端零星热应激的适应性和延续效应","authors":"John Roberts Padde , Yinghui Zhou , Yunxuan Chen , Yuxiao Zhu , Yuxuan Yang , Min Hou , Lu Chen , Zhipeng Xu , Donghui Zhang , Lin Chen , MinJun Ji","doi":"10.1016/j.actatropica.2024.107417","DOIUrl":null,"url":null,"abstract":"<div><div>Mosquitoes, as temperature-sensitive ectothermic vectors, exhibit temperature-dependence. This study investigates <em>Culex pipiens pallens (Cx. pallens</em>) responses to abrupt temperature increases and their implications on mosquito physiology.</div><div>First instar larvae (24hr post hatching) and newly enclosed adults (24hr post emergence) were separately exposed to heat shock regimes of 33°C, 37°C, and 42°C for 3 days alongside a control temperature of 27°C. Results showed that mortality was triggered at 42°C within a day. Adult male mosquitoes were less tolerant to all temperatures than larvae and adult females (<em>p < 0.05</em>). Exposing larvae to constant temperatures for 3 days significantly decreased larvae's development time, growth rate and adult emergence (<em>p</em> < <em>0.05</em>). Reproductive fitness was significantly reduced (<em>p < 0.05</em>) in males emerging from larvae exposed to 37°C. Life table parameters showed significant increased mortality rate, kill power and decreased life expectancy at the embryonic stage (<em>p < 0.05</em>). Furthermore, heatwaves deactivated the Transient receptor protein ankyrin 1 at 37°C (<em>p</em> < <em>0.05</em>) in larvae but not adults. Calmodium, Heat shock protein 90, and small heat shock protein expression were significantly decreased in larvae at 37°C (<em>p < 0.05</em>) as compared to larvae raised at 33°C and 27°C.</div><div>In conclusion, we classified the heat waves into three categories: adaptable (33°C), critical (37°C), and fatal (42°C). Prolonged exposure of <em>Culex pallens</em> larvae to extreme heat affects the male reproductive output. These findings may serve as an important reference for forecasting vector and pest dynamics and used to tailor mosquito prevention and control measures.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Adaptation and Carry Over Effects of Extreme Sporadic Heat Stress in Culex Mosquitoes.\",\"authors\":\"John Roberts Padde , Yinghui Zhou , Yunxuan Chen , Yuxiao Zhu , Yuxuan Yang , Min Hou , Lu Chen , Zhipeng Xu , Donghui Zhang , Lin Chen , MinJun Ji\",\"doi\":\"10.1016/j.actatropica.2024.107417\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Mosquitoes, as temperature-sensitive ectothermic vectors, exhibit temperature-dependence. This study investigates <em>Culex pipiens pallens (Cx. pallens</em>) responses to abrupt temperature increases and their implications on mosquito physiology.</div><div>First instar larvae (24hr post hatching) and newly enclosed adults (24hr post emergence) were separately exposed to heat shock regimes of 33°C, 37°C, and 42°C for 3 days alongside a control temperature of 27°C. Results showed that mortality was triggered at 42°C within a day. Adult male mosquitoes were less tolerant to all temperatures than larvae and adult females (<em>p < 0.05</em>). Exposing larvae to constant temperatures for 3 days significantly decreased larvae's development time, growth rate and adult emergence (<em>p</em> < <em>0.05</em>). Reproductive fitness was significantly reduced (<em>p < 0.05</em>) in males emerging from larvae exposed to 37°C. Life table parameters showed significant increased mortality rate, kill power and decreased life expectancy at the embryonic stage (<em>p < 0.05</em>). Furthermore, heatwaves deactivated the Transient receptor protein ankyrin 1 at 37°C (<em>p</em> < <em>0.05</em>) in larvae but not adults. Calmodium, Heat shock protein 90, and small heat shock protein expression were significantly decreased in larvae at 37°C (<em>p < 0.05</em>) as compared to larvae raised at 33°C and 27°C.</div><div>In conclusion, we classified the heat waves into three categories: adaptable (33°C), critical (37°C), and fatal (42°C). Prolonged exposure of <em>Culex pallens</em> larvae to extreme heat affects the male reproductive output. These findings may serve as an important reference for forecasting vector and pest dynamics and used to tailor mosquito prevention and control measures.</div></div>\",\"PeriodicalId\":7240,\"journal\":{\"name\":\"Acta tropica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-10-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta tropica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0001706X24002985\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta tropica","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0001706X24002985","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Adaptation and Carry Over Effects of Extreme Sporadic Heat Stress in Culex Mosquitoes.
Mosquitoes, as temperature-sensitive ectothermic vectors, exhibit temperature-dependence. This study investigates Culex pipiens pallens (Cx. pallens) responses to abrupt temperature increases and their implications on mosquito physiology.
First instar larvae (24hr post hatching) and newly enclosed adults (24hr post emergence) were separately exposed to heat shock regimes of 33°C, 37°C, and 42°C for 3 days alongside a control temperature of 27°C. Results showed that mortality was triggered at 42°C within a day. Adult male mosquitoes were less tolerant to all temperatures than larvae and adult females (p < 0.05). Exposing larvae to constant temperatures for 3 days significantly decreased larvae's development time, growth rate and adult emergence (p < 0.05). Reproductive fitness was significantly reduced (p < 0.05) in males emerging from larvae exposed to 37°C. Life table parameters showed significant increased mortality rate, kill power and decreased life expectancy at the embryonic stage (p < 0.05). Furthermore, heatwaves deactivated the Transient receptor protein ankyrin 1 at 37°C (p < 0.05) in larvae but not adults. Calmodium, Heat shock protein 90, and small heat shock protein expression were significantly decreased in larvae at 37°C (p < 0.05) as compared to larvae raised at 33°C and 27°C.
In conclusion, we classified the heat waves into three categories: adaptable (33°C), critical (37°C), and fatal (42°C). Prolonged exposure of Culex pallens larvae to extreme heat affects the male reproductive output. These findings may serve as an important reference for forecasting vector and pest dynamics and used to tailor mosquito prevention and control measures.
期刊介绍:
Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.