库蚊对极端零星热应激的适应性和延续效应

IF 2.1 3区 医学 Q2 PARASITOLOGY Acta tropica Pub Date : 2024-10-09 DOI:10.1016/j.actatropica.2024.107417
John Roberts Padde , Yinghui Zhou , Yunxuan Chen , Yuxiao Zhu , Yuxuan Yang , Min Hou , Lu Chen , Zhipeng Xu , Donghui Zhang , Lin Chen , MinJun Ji
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引用次数: 0

摘要

蚊子作为对温度敏感的体外温媒,表现出温度依赖性。本研究调查了库蚊(Cx. pipiens pallens)对温度骤然升高的反应及其对蚊子生理的影响。将第一龄幼虫(孵化后 24 小时)和新孵化的成虫(出壳后 24 小时)分别暴露在 33°C、37°C 和 42°C 的热冲击环境中 3 天,同时将温度控制在 27°C。结果表明,在 42°C 的温度下,蚊子在一天之内就会死亡。与幼虫和成年雌蚊相比,成年雄蚊对所有温度的耐受性都较差(p < 0.05)。将幼虫暴露在恒温中 3 天会显著减少幼虫的发育时间、生长速度和成虫出现(p < 0.05)。暴露在 37°C 温度下的幼虫所产雄性成虫的生殖能力明显降低(p < 0.05)。生命表参数显示,胚胎阶段的死亡率、杀伤力和预期寿命明显增加(p < 0.05)。此外,37°C的热浪会使幼虫的瞬时受体蛋白ankyrin 1失活(p < 0.05),但不会使成虫的瞬时受体蛋白ankyrin 1失活。与 33°C 和 27°C 的幼虫相比,37°C 的幼虫体内钙钠蛋白、热休克蛋白 90 和小热休克蛋白的表达明显减少(p < 0.05)。总之,我们将热浪分为三类:适应性热浪(33°C)、临界热浪(37°C)和致命热浪(42°C)。库蚊幼虫长期暴露在极端高温下会影响雄性的生殖输出。这些发现可作为预测病媒和害虫动态的重要参考,并用于制定蚊虫预防和控制措施。
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Adaptation and Carry Over Effects of Extreme Sporadic Heat Stress in Culex Mosquitoes.
Mosquitoes, as temperature-sensitive ectothermic vectors, exhibit temperature-dependence. This study investigates Culex pipiens pallens (Cx. pallens) responses to abrupt temperature increases and their implications on mosquito physiology.
First instar larvae (24hr post hatching) and newly enclosed adults (24hr post emergence) were separately exposed to heat shock regimes of 33°C, 37°C, and 42°C for 3 days alongside a control temperature of 27°C. Results showed that mortality was triggered at 42°C within a day. Adult male mosquitoes were less tolerant to all temperatures than larvae and adult females (p < 0.05). Exposing larvae to constant temperatures for 3 days significantly decreased larvae's development time, growth rate and adult emergence (p < 0.05). Reproductive fitness was significantly reduced (p < 0.05) in males emerging from larvae exposed to 37°C. Life table parameters showed significant increased mortality rate, kill power and decreased life expectancy at the embryonic stage (p < 0.05). Furthermore, heatwaves deactivated the Transient receptor protein ankyrin 1 at 37°C (p < 0.05) in larvae but not adults. Calmodium, Heat shock protein 90, and small heat shock protein expression were significantly decreased in larvae at 37°C (p < 0.05) as compared to larvae raised at 33°C and 27°C.
In conclusion, we classified the heat waves into three categories: adaptable (33°C), critical (37°C), and fatal (42°C). Prolonged exposure of Culex pallens larvae to extreme heat affects the male reproductive output. These findings may serve as an important reference for forecasting vector and pest dynamics and used to tailor mosquito prevention and control measures.
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来源期刊
Acta tropica
Acta tropica 医学-寄生虫学
CiteScore
5.40
自引率
11.10%
发文量
383
审稿时长
37 days
期刊介绍: Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.
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