双酚 A 对新生大鼠卵泡发育过程中 Notch(Notch2 和 Jagged2)信号通路的影响

IF 1.6 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnic & Histochemistry Pub Date : 2024-10-09 DOI:10.1080/10520295.2024.2361313
Özlem Özden Akkaya, Artay Yağci, Berrin Zik, A S M Golam Kibria, Sabire Güler, Sefa Çelik, Korhan Altunbaş
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引用次数: 0

摘要

原始卵泡的形成决定了卵巢中卵泡池的大小,对女性的生殖能力至关重要。卵母细胞巢的破裂和原始卵泡的形成在很大程度上取决于卵母细胞与周围前浆膜细胞之间的交流。神经原位点缺口同源蛋白(Notch)信号通路是这种细胞间通信的关键角色,负责原始卵泡的形成。然而,不同的内分泌干扰物,包括双酚 A(BPA,一种增塑剂和可重复使用塑料容器的成分)可能会影响 Notch 信号通路,并可能通过 Notch 信号通路诱发卵巢功能障碍。因此,我们研究了双酚 A 处理对卵巢中卵泡期的比例分布、卵泡数量、凋亡水平以及 Notch2 和 Jagged2 表达的可能影响。在体内新生儿期和胎儿期,以50微克/千克/天或50毫克/千克/天的剂量和不同的时间间隔注射双酚A。收集各实验组的卵巢后,对卵泡进行计数,并通过 TUNEL 试验测定卵泡凋亡的频率。此外,还通过免疫组化染色和 qPCR 评估了 Notch2 和 Jagged2 的表达。总之,双酚A处理会影响卵泡数量和凋亡水平,Notch2和Jagged2的表达随卵泡阶段而变化。还观察到这些参数与双酚 A 暴露的剂量和时间有关。
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The effect of bisphenol A on the Notch (Notch2 and Jagged2) signaling pathway in the follicular development of the neonatal rat ovary.

The formation of primordial follicles determines the pool size of follicles in the ovary, and is crucial for female reproductivity. Oocyte nest breakdown, and the formation of primordial follicles, largely depend upon the communication between oocytes and the surrounding pregranulosa cells. The neurogenic locus notch homolog protein (Notch) signaling pathway is the key player for this cell-to-cell communication, and is responsible for primordial folliculogenesis. However, different endocrine disruptors, including bisphenol A (BPA; a plasticizer and a constituent of reusable plastic containers) may affect the Notch signaling pathway, and might induce ovary dysfunction via Notch signaling. Consequently, we investigated the possible influence of BPA treatment on the proportional distribution of the follicular stages, follicle numbers, levels of apoptosis, and on Notch2 and Jagged2 expressions in the ovary. BPA was administered at doses of either 50 µg/kg/day or 50 mg/kg/day, at different time intervals, during neonatal and fetal periods in vivo. After collecting the ovaries from the various experimental groups, follicles were counted, and frequency of apoptosis was determined by TUNEL assay. In addition, Notch2 and Jagged2 expressions were assessed by immunohistochemical staining and qPCR. In summary, BPA treatment affected the follicle numbers and apoptosis level, and Notch2 and Jagged2 expressions varied with follicular stage. It was also observed that these parameters were dose and time dependent with respect to BPA exposure.

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来源期刊
Biotechnic & Histochemistry
Biotechnic & Histochemistry 生物-生物工程与应用微生物
CiteScore
3.40
自引率
6.20%
发文量
46
审稿时长
6-12 weeks
期刊介绍: Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.
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