{"title":"斯里兰卡甘帕哈地区库蚊中肠共生细菌的多样性和物种组成","authors":"Randi Hathnagoda, Pinidi Gunathilake, Thilini Buddhinee, Pabasara Welgama, Hasini Gunarathna, Harshani Perera, Koshila Ranasinghe","doi":"10.1155/2024/1832200","DOIUrl":null,"url":null,"abstract":"<p><p>Mosquitoes, notorious for their deadly impact as disease vectors, also hold economic value owing to their roles in disease transmission. The present study focuses on the importance of understanding mosquito gut microbiota for implementing innovative vector control strategies, thereby mitigating disease transmission. The study was conducted in the Gampaha Medical Office of Health (MOH) area of Sri Lanka with the focus of elucidating the microbial diversity within the midgut of <i>Culex quinquefasciatus</i>, a crucial step to support ongoing paratransgenesis efforts. Sampling was performed by utilizing standard mosquito sampling techniques and their midgut homogenates were plated on Plate Count Agar to isolate bacteria, which were then identified through biochemical tests. Subsequently, the most abundant bacterial families were subjected to DNA extraction, PCR amplification, and gene sequencing for species identification. The study revealed the presence of four bacterial families (Staphylococcaceae, Streptococcaceae, Neisseriaceae, and Moraxellaceae) in adult mosquitoes, while larvae harbored an additional family, Micrococcaceae. Interestingly, the relative distribution of midgut bacteria varied significantly among field-caught larval and adult strains from different study areas (chi-square = 1.673; <i>P</i> < 0.05), indicating similar bacterial flora across mosquito life stages and geographical locations. Of particular interest is the identification of <i>Lysinibacillus sphaericus</i>, a bacterium with potential for paratransgenesis applications. Given the high mosquito density in the study area, leveraging paratransgenesis for <i>Cx. quinquefasciatus</i> control is recommended. Furthermore, insights into gut microbes could inform the integration of gut microflora from modified strains into existing Sterile Insect Technique (SIT) and Incompatible Insect Technique (IIT) approaches in Sri Lanka.</p>","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"2024 ","pages":"1832200"},"PeriodicalIF":2.1000,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11458302/pdf/","citationCount":"0","resultStr":"{\"title\":\"Diversity and Species Composition of Midgut Symbiotic Bacteria in <i>Culex quinquefasciatus</i> Mosquitoes in Gampaha District, Sri Lanka.\",\"authors\":\"Randi Hathnagoda, Pinidi Gunathilake, Thilini Buddhinee, Pabasara Welgama, Hasini Gunarathna, Harshani Perera, Koshila Ranasinghe\",\"doi\":\"10.1155/2024/1832200\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Mosquitoes, notorious for their deadly impact as disease vectors, also hold economic value owing to their roles in disease transmission. 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The study revealed the presence of four bacterial families (Staphylococcaceae, Streptococcaceae, Neisseriaceae, and Moraxellaceae) in adult mosquitoes, while larvae harbored an additional family, Micrococcaceae. Interestingly, the relative distribution of midgut bacteria varied significantly among field-caught larval and adult strains from different study areas (chi-square = 1.673; <i>P</i> < 0.05), indicating similar bacterial flora across mosquito life stages and geographical locations. Of particular interest is the identification of <i>Lysinibacillus sphaericus</i>, a bacterium with potential for paratransgenesis applications. Given the high mosquito density in the study area, leveraging paratransgenesis for <i>Cx. quinquefasciatus</i> control is recommended. 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引用次数: 0
摘要
蚊子是臭名昭著的致命病媒,但由于其在疾病传播中的作用,也具有经济价值。本研究的重点是了解蚊子肠道微生物群对实施创新病媒控制策略的重要性,从而减少疾病传播。这项研究在斯里兰卡甘帕哈卫生医疗办公室(MOH)地区进行,重点是阐明五带喙库蚊中肠微生物的多样性,这是支持正在进行的副传代工作的关键一步。利用标准的蚊子取样技术进行取样,将它们的中肠匀浆培养在平板计数琼脂上以分离细菌,然后通过生化测试进行鉴定。随后,对数量最多的细菌家族进行 DNA 提取、PCR 扩增和基因测序,以确定物种。研究发现,成蚊体内存在四个细菌科(葡萄球菌科、链球菌科、奈瑟氏菌科和莫拉菌科),而幼虫体内则有一个细菌科,即小球菌科。有趣的是,中肠细菌的相对分布在不同研究地区的野外捕获幼虫和成蚊菌株之间有显著差异(chi-square = 1.673; P < 0.05),表明蚊子不同生命阶段和不同地理位置的细菌群相似。尤其令人感兴趣的是,发现了一种具有副基因应用潜力的细菌--Lysinibacillus sphaericus。鉴于研究地区的蚊子密度很高,建议利用副转化技术来控制五步蛇。此外,对肠道微生物的了解可以为将改良菌株的肠道微生物菌群纳入斯里兰卡现有的昆虫不育技术(SIT)和昆虫不相容技术(IIT)方法提供信息。
Diversity and Species Composition of Midgut Symbiotic Bacteria in Culex quinquefasciatus Mosquitoes in Gampaha District, Sri Lanka.
Mosquitoes, notorious for their deadly impact as disease vectors, also hold economic value owing to their roles in disease transmission. The present study focuses on the importance of understanding mosquito gut microbiota for implementing innovative vector control strategies, thereby mitigating disease transmission. The study was conducted in the Gampaha Medical Office of Health (MOH) area of Sri Lanka with the focus of elucidating the microbial diversity within the midgut of Culex quinquefasciatus, a crucial step to support ongoing paratransgenesis efforts. Sampling was performed by utilizing standard mosquito sampling techniques and their midgut homogenates were plated on Plate Count Agar to isolate bacteria, which were then identified through biochemical tests. Subsequently, the most abundant bacterial families were subjected to DNA extraction, PCR amplification, and gene sequencing for species identification. The study revealed the presence of four bacterial families (Staphylococcaceae, Streptococcaceae, Neisseriaceae, and Moraxellaceae) in adult mosquitoes, while larvae harbored an additional family, Micrococcaceae. Interestingly, the relative distribution of midgut bacteria varied significantly among field-caught larval and adult strains from different study areas (chi-square = 1.673; P < 0.05), indicating similar bacterial flora across mosquito life stages and geographical locations. Of particular interest is the identification of Lysinibacillus sphaericus, a bacterium with potential for paratransgenesis applications. Given the high mosquito density in the study area, leveraging paratransgenesis for Cx. quinquefasciatus control is recommended. Furthermore, insights into gut microbes could inform the integration of gut microflora from modified strains into existing Sterile Insect Technique (SIT) and Incompatible Insect Technique (IIT) approaches in Sri Lanka.
期刊介绍:
Journal of Tropical Medicine is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on all aspects of tropical diseases. Articles on the pathology, diagnosis, and treatment of tropical diseases, parasites and their hosts, epidemiology, and public health issues will be considered. Journal of Tropical Medicine aims to facilitate the communication of advances addressing global health and mortality relating to tropical diseases.