盘基竹荪的抗菌效应器:对不同细菌种类的特异性活性。

IF 3.7 2区 生物学 Q2 MICROBIOLOGY mSphere Pub Date : 2024-10-29 Epub Date: 2024-10-08 DOI:10.1128/msphere.00471-24
Raphael Munoz-Ruiz, Otmane Lamrabet, Tania Jauslin, Cyril Guilhen, Alixia Bourbon, Pierre Cosson
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引用次数: 0

摘要

盘基变形虫(Dictyostelium discoideum)是一种吞噬型变形虫,能不断吞噬、杀死和消化细菌。以前的研究发现,盘基变形虫细胞提取物具有对肺炎克雷伯氏菌有效的溶菌活性。在本研究中,我们研究了盘尾丝虫细胞提取物对五种不同细菌(肺炎克雷伯菌、大肠杆菌、绿脓杆菌、金黄色葡萄球菌和枯草杆菌)的细菌分解活性。我们首先分析了在不同 pH 值范围内对这五种细菌的平行细菌溶解活性。然后,我们测量了盘状芽孢杆菌 kil1 和 modA 基因敲除突变体的剩余溶菌活性。我们还对盘菌提取物进行了部分分馏,并评估了其对不同细菌的活性。我们的研究结果表明,对不同细菌的最佳溶菌活性来自于不同效应器的作用。通过蛋白质组分析,我们提出了一份潜在的溶菌作用因子清单。在过去的几十年中,许多抗菌作用因子都已被鉴定,但它们的生物学重要性、作用方式和特异性往往仍在研究之中。在这里,我们研究了盘尾丝藻提取物对五种革兰氏阴性和革兰氏阳性细菌的体外溶菌活性。我们的研究结果表明,不同细菌的最佳裂解作用需要调动不同的效应因子。蛋白质组分析产生了一份潜在的细菌溶解效应物清单。这项工作为今后分析盘尾丝虫活细胞中单个效应器的作用开辟了道路。
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Antibacterial effectors in Dictyostelium discoideum: specific activity against different bacterial species.

Dictyostelium discoideum is a phagocytic amoeba continuously eating, killing, and digesting bacteria. Previous studies have detected in D. discoideum cell extracts a bacteriolytic activity effective against Klebsiella pneumoniae bacteria. In this study, we characterized bacteriolytic activities found in D. discoideum cell extracts against five different bacteria (K. pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtilis). We first analyzed the bacteriolytic activity against these five bacteria in parallel over a range of pH values. We then measured the remaining bacteriolytic activity in D. discoideum kil1 and modA knockout mutants. We also performed partial fractionation of D. discoideum extracts and assessed activity against different bacteria. Together our results indicate that optimal bacteriolytic activity against different bacteria results from the action of different effectors. Proteomic analysis allowed us to propose a list of potential bacteriolytic effectors.IMPORTANCEMany antibacterial effectors have been characterized over the past decades, and their biological importance, mode of action, and specificity are often still under study. Here we characterized in vitro bacteriolytic activity in D. discoideum extracts against five species of Gram-negative and Gram-positive bacteria. Our results reveal that optimal lysis of different bacteria mobilizes different effectors. Proteomic analysis generated a list of potential bacteriolytic effectors. This work opens the way for future analysis of the role of individual effectors in living D. discoideum cells.

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来源期刊
mSphere
mSphere Immunology and Microbiology-Microbiology
CiteScore
8.50
自引率
2.10%
发文量
192
审稿时长
11 weeks
期刊介绍: mSphere™ is a multi-disciplinary open-access journal that will focus on rapid publication of fundamental contributions to our understanding of microbiology. Its scope will reflect the immense range of fields within the microbial sciences, creating new opportunities for researchers to share findings that are transforming our understanding of human health and disease, ecosystems, neuroscience, agriculture, energy production, climate change, evolution, biogeochemical cycling, and food and drug production. Submissions will be encouraged of all high-quality work that makes fundamental contributions to our understanding of microbiology. mSphere™ will provide streamlined decisions, while carrying on ASM''s tradition for rigorous peer review.
期刊最新文献
Erratum for Choi et al., "Human saliva modifies growth, biofilm architecture, and competitive behaviors of oral streptococci". mSphere of Influence: Venturing outside the biology canon with sex and gender. Pneumococcal extracellular vesicles mediate horizontal gene transfer via the transformation machinery. Adaptation to zinc restriction in Streptococcus agalactiae: role of the ribosomal protein and zinc-importers regulated by AdcR. Analytical measuring interval, linearity, and precision of serology assays for detection of SARS-CoV-2 antibodies according to CLSI guidelines.
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