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引用次数: 0
摘要
与大多数粘合剂不同,生物捕捉键会随着张力的增加而增强。这一特性对于特定的受体-配体相互作用至关重要,是生物粘附动力学、细胞通讯和机械传感的基础。虽然已经构想出了人工捕捉键,但其捕捉行为的可调性却很有限。在这里,我们展示了鱼钩,这是一种经过合理设计的 DNA 捕获键,可以根据各种捕获行为进行微调。我们开发了设计这些 DNA 结构的模型,并通过单分子力谱仪对不同的捕捉行为进行了实验验证。鱼钩结构支持广阔的序列依赖行为空间,使其成为重新编程生物相互作用和工程力强化材料的重要工具。
Unlike most adhesive bonds, biological catch bonds strengthen with increased tension. This characteristic is essential to specific receptor-ligand interactions, underpinning biological adhesion dynamics, cell communication, and mechanosensing. While artificial catch bonds have been conceived, the tunability of their catch behaviour is limited. Here, we present the fish-hook, a rationally designed DNA catch bond that can be finely adjusted to a wide range of catch behaviours. We develop models to design these DNA structures and experimentally validate different catch behaviours by single-molecule force spectroscopy. The fish-hook architecture supports a vast sequence-dependent behaviour space, making it a valuable tool for reprogramming biological interactions and engineering force-strengthening materials.
期刊介绍:
Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.