基于 ZIF-8 纳米粒子包裹的 dsRNA 对 Nilaparvata lugens (Stål) 中 CYP303A1 及其潜在应用的表征

IF 3.8 1区农林科学 Q1 AGRONOMY Pest Management Science Pub Date : 2024-10-12 DOI:10.1002/ps.8479

Zuyi Du, Guijian Zhang, Chang Yu, Yao Qin, Shun He, Jianhong Li, Le Guo, Hu Wan

{"title":"基于 ZIF-8 纳米粒子包裹的 dsRNA 对 Nilaparvata lugens (Stål) 中 CYP303A1 及其潜在应用的表征","authors":"Zuyi Du, Guijian Zhang, Chang Yu, Yao Qin, Shun He, Jianhong Li, Le Guo, Hu Wan","doi":"10.1002/ps.8479","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> BACKGROUND</h3>\n \n <p>RNA interference (RNAi) technology has been put forward as a promising method for pest control and resistance management. Mining highly efficient lethal genes and constructing stable double-stranded RNA (dsRNA) delivery systems are of great significance to improve the application potential of RNAi technology.</p>\n </section>\n \n <section>\n \n <h3> RESULTS</h3>\n \n <p>In this study, we characterized a molting-related gene, <i>NlCYP303A1</i>, in <i>Nilaparvata lugens</i> that was highly expressed in the cuticle and at the end stages of each instar in nymphs. Silencing the expression of <i>NlCYP303A1</i> in <i>N. lugens</i> resulted in a deformed phenotype and a significant increase in mortality. Furthermore, interfering with <i>NlCYP303A1</i> changed the relative expression of key genes in the chitin synthesis and degradation pathway. Finally, we used the nanocarrier zeolitic imidazolate framework-8 (ZIF-8) to load ds<i>NlCYP303A1</i>, forming a complex denoted as ds<i>NlCYP303A1</i>@ZIF-8. The results of both feeding and rice-seedling dip experiments indicated that the expression of <i>NlCYP303A1</i> was dramatically and persistently suppressed by the ds<i>NlCYP303A1</i>@ZIF-8 treatment, compared with treatment with ds<i>NlCYP303A1</i>, suggesting that ZIF-8 can enhance the interference efficiency as well as the stability of ds<i>NlCYP303A1</i>.</p>\n </section>\n \n <section>\n \n <h3> CONCLUSIONS</h3>\n \n <p>Our results demonstrate that the lethal gene <i>NlCYP303A1</i> can be employed as an excellent target for RNAi technology by loading onto a nano-delivery system, and provide new insights into the creation of innovative pest control approaches. © 2024 Society of Chemical Industry.</p>\n </section>\n </div>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"81 2","pages":"766-776"},"PeriodicalIF":3.8000,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Characterization of CYP303A1 and its potential application based on ZIF-8 nanoparticle-wrapped dsRNA in Nilaparvata lugens (Stål)\",\"authors\":\"Zuyi Du, Guijian Zhang, Chang Yu, Yao Qin, Shun He, Jianhong Li, Le Guo, Hu Wan\",\"doi\":\"10.1002/ps.8479\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> BACKGROUND</h3>\\n \\n <p>RNA interference (RNAi) technology has been put forward as a promising method for pest control and resistance management. Mining highly efficient lethal genes and constructing stable double-stranded RNA (dsRNA) delivery systems are of great significance to improve the application potential of RNAi technology.</p>\\n </section>\\n \\n <section>\\n \\n <h3> RESULTS</h3>\\n \\n <p>In this study, we characterized a molting-related gene, <i>NlCYP303A1</i>, in <i>Nilaparvata lugens</i> that was highly expressed in the cuticle and at the end stages of each instar in nymphs. Silencing the expression of <i>NlCYP303A1</i> in <i>N. lugens</i> resulted in a deformed phenotype and a significant increase in mortality. Furthermore, interfering with <i>NlCYP303A1</i> changed the relative expression of key genes in the chitin synthesis and degradation pathway. Finally, we used the nanocarrier zeolitic imidazolate framework-8 (ZIF-8) to load ds<i>NlCYP303A1</i>, forming a complex denoted as ds<i>NlCYP303A1</i>@ZIF-8. The results of both feeding and rice-seedling dip experiments indicated that the expression of <i>NlCYP303A1</i> was dramatically and persistently suppressed by the ds<i>NlCYP303A1</i>@ZIF-8 treatment, compared with treatment with ds<i>NlCYP303A1</i>, suggesting that ZIF-8 can enhance the interference efficiency as well as the stability of ds<i>NlCYP303A1</i>.</p>\\n </section>\\n \\n <section>\\n \\n <h3> CONCLUSIONS</h3>\\n \\n <p>Our results demonstrate that the lethal gene <i>NlCYP303A1</i> can be employed as an excellent target for RNAi technology by loading onto a nano-delivery system, and provide new insights into the creation of innovative pest control approaches. © 2024 Society of Chemical Industry.</p>\\n </section>\\n </div>\",\"PeriodicalId\":218,\"journal\":{\"name\":\"Pest Management Science\",\"volume\":\"81 2\",\"pages\":\"766-776\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2024-10-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pest Management Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.8479\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pest Management Science","FirstCategoryId":"97","ListUrlMain":"https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.8479","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

摘要

RNA 干扰（RNAi）技术被认为是一种前景广阔的害虫控制和抗性管理方法。挖掘高效致死基因和构建稳定的双链 RNA（dsRNA）传递系统对于提高 RNAi 技术的应用潜力具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

摘要图片

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

Characterization of CYP303A1 and its potential application based on ZIF-8 nanoparticle-wrapped dsRNA in Nilaparvata lugens (Stål)

BACKGROUND

RNA interference (RNAi) technology has been put forward as a promising method for pest control and resistance management. Mining highly efficient lethal genes and constructing stable double-stranded RNA (dsRNA) delivery systems are of great significance to improve the application potential of RNAi technology.

RESULTS

In this study, we characterized a molting-related gene, NlCYP303A1, in Nilaparvata lugens that was highly expressed in the cuticle and at the end stages of each instar in nymphs. Silencing the expression of NlCYP303A1 in N. lugens resulted in a deformed phenotype and a significant increase in mortality. Furthermore, interfering with NlCYP303A1 changed the relative expression of key genes in the chitin synthesis and degradation pathway. Finally, we used the nanocarrier zeolitic imidazolate framework-8 (ZIF-8) to load dsNlCYP303A1, forming a complex denoted as dsNlCYP303A1@ZIF-8. The results of both feeding and rice-seedling dip experiments indicated that the expression of NlCYP303A1 was dramatically and persistently suppressed by the dsNlCYP303A1@ZIF-8 treatment, compared with treatment with dsNlCYP303A1, suggesting that ZIF-8 can enhance the interference efficiency as well as the stability of dsNlCYP303A1.

CONCLUSIONS

Our results demonstrate that the lethal gene NlCYP303A1 can be employed as an excellent target for RNAi technology by loading onto a nano-delivery system, and provide new insights into the creation of innovative pest control approaches. © 2024 Society of Chemical Industry.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.