造成蒂尔基耶东北部猕猴桃藤蔓衰退和根腐病的阿米拉里亚种:猕猴桃健康日益受到关注

IF 1.1 4区 农林科学 Q3 PLANT SCIENCES Journal of Phytopathology Pub Date : 2024-10-10 DOI:10.1111/jph.13409
Muharrem Türkkan, Tuğba Bozoğlu, Sibel Derviş, İsmail Erper, Göksel Özer
{"title":"造成蒂尔基耶东北部猕猴桃藤蔓衰退和根腐病的阿米拉里亚种:猕猴桃健康日益受到关注","authors":"Muharrem Türkkan,&nbsp;Tuğba Bozoğlu,&nbsp;Sibel Derviş,&nbsp;İsmail Erper,&nbsp;Göksel Özer","doi":"10.1111/jph.13409","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Kiwifruit (<i>Actinidia deliciosa</i>) production in northeastern Türkiye is facing an emerging threat from Armillaria root rot. Surveys conducted in August 2021 and 2022 revealed that 10.5%–17.5% of the 200–500 vines within each of 35 kiwifruit orchards in the Perşembe, Gülyalı and Altınordu districts of Ordu province exhibited symptoms of Armillaria root rot, including complete defoliation. Diseased plants exhibited diagnostic signs on their root collars and woody roots, including white mycelial fans, extensive wood rot and rhizomorphs. A single <i>Armillaria</i> isolate was obtained from each of the 35 symptomatic orchards. Molecular identification, employing DNA sequencing of the large subunit (LSU) region of rDNA, the translation elongation factor subunit 1-alpha (<i>TEF1</i>) gene and the second largest RNA polymerase II B-subunit (<i>RPB2</i>) gene, identified 20 isolates as <i>Armillaria gallica</i> and 15 as <i>A</i>. <i>mellea</i>. Phylogenetic analyses, based on <i>TEF1</i> alignments, further supported the species identification with a 100% bootstrap value. To examine genetic diversity, start codon targeted (SCoT) marker 13 was used, successfully differentiating the two <i>Armillaria</i> species and revealing three distinct clades. Clades I and II comprised 15 and 5 isolates of <i>A</i>. <i>gallica</i>, respectively, while Clade III contained all 15 <i>A</i>. <i>mellea</i> isolates. Pathogenicity testing on kiwifruit seedlings (cv. Hayward) revealed the ability of both <i>A</i>. <i>gallica</i> and <i>A</i>. <i>mellea</i> isolates to induce disease. Rhizomorph-producing isolates in kiwifruit seedlings displayed high virulence, inducing severe leaf necrosis and seedling mortality, while isolates lacking these structures were non-pathogenic. This study identifies the first global instance of <i>A</i>. <i>gallica</i> causing kiwifruit root rot. This finding, along with the identification of <i>A</i>. <i>mellea</i> as a causal agent in the country, highlights the emerging threat of Armillaria root rot to kiwifruit production in the region.</p>\n </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Armillaria Species Causing Kiwifruit Vine Decline and Root Rot in Northeastern Türkiye: A Growing Concern for Kiwifruit Health\",\"authors\":\"Muharrem Türkkan,&nbsp;Tuğba Bozoğlu,&nbsp;Sibel Derviş,&nbsp;İsmail Erper,&nbsp;Göksel Özer\",\"doi\":\"10.1111/jph.13409\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>Kiwifruit (<i>Actinidia deliciosa</i>) production in northeastern Türkiye is facing an emerging threat from Armillaria root rot. Surveys conducted in August 2021 and 2022 revealed that 10.5%–17.5% of the 200–500 vines within each of 35 kiwifruit orchards in the Perşembe, Gülyalı and Altınordu districts of Ordu province exhibited symptoms of Armillaria root rot, including complete defoliation. Diseased plants exhibited diagnostic signs on their root collars and woody roots, including white mycelial fans, extensive wood rot and rhizomorphs. A single <i>Armillaria</i> isolate was obtained from each of the 35 symptomatic orchards. Molecular identification, employing DNA sequencing of the large subunit (LSU) region of rDNA, the translation elongation factor subunit 1-alpha (<i>TEF1</i>) gene and the second largest RNA polymerase II B-subunit (<i>RPB2</i>) gene, identified 20 isolates as <i>Armillaria gallica</i> and 15 as <i>A</i>. <i>mellea</i>. Phylogenetic analyses, based on <i>TEF1</i> alignments, further supported the species identification with a 100% bootstrap value. To examine genetic diversity, start codon targeted (SCoT) marker 13 was used, successfully differentiating the two <i>Armillaria</i> species and revealing three distinct clades. Clades I and II comprised 15 and 5 isolates of <i>A</i>. <i>gallica</i>, respectively, while Clade III contained all 15 <i>A</i>. <i>mellea</i> isolates. Pathogenicity testing on kiwifruit seedlings (cv. Hayward) revealed the ability of both <i>A</i>. <i>gallica</i> and <i>A</i>. <i>mellea</i> isolates to induce disease. Rhizomorph-producing isolates in kiwifruit seedlings displayed high virulence, inducing severe leaf necrosis and seedling mortality, while isolates lacking these structures were non-pathogenic. This study identifies the first global instance of <i>A</i>. <i>gallica</i> causing kiwifruit root rot. This finding, along with the identification of <i>A</i>. <i>mellea</i> as a causal agent in the country, highlights the emerging threat of Armillaria root rot to kiwifruit production in the region.</p>\\n </div>\",\"PeriodicalId\":16843,\"journal\":{\"name\":\"Journal of Phytopathology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2024-10-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Phytopathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jph.13409\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jph.13409","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

土耳其东北部的猕猴桃(Actinidia deliciosa)生产正面临着新出现的根腐病威胁。2021 年 8 月和 2022 年 8 月进行的调查显示,在奥尔杜省佩尔森贝、居利亚勒和阿尔特诺尔杜地区的 35 个猕猴桃果园中,每个果园的 200-500 株葡萄藤中有 10.5%-17.5% 出现了根腐病的症状,包括完全落叶。病株的根领和木质根上出现了诊断性症状,包括白色菌丝扇、大面积木质腐烂和根瘤。从 35 个有症状的果园中,每个果园都获得了一个阿米拉氏菌分离株。通过对 rDNA 大亚基(LSU)区域、翻译延伸因子亚基 1-α(TEF1)基因和第二大 RNA 聚合酶 II B-亚基(RPB2)基因进行 DNA 测序进行分子鉴定,确定 20 个分离株为五倍子臂孢属(Armillaria gallica),15 个分离株为梅勒氏臂孢属(A. mellea)。基于 TEF1 的系统发育分析进一步支持了物种鉴定,引导值达到 100%。为了研究遗传多样性,使用了起始密码子目标(SCoT)标记 13,成功地区分了这两种节肢动物,并发现了三个不同的支系。支系 I 和支系 II 分别包含 15 个和 5 个 A. gallica 分离物,而支系 III 则包含所有 15 个 A. mellea 分离物。对猕猴桃幼苗(变种 Hayward)的致病性测试表明,A. gallica 和 A. mellea 分离物都有诱发疾病的能力。在猕猴桃幼苗中产生根状茎的分离株显示出很强的致病力,可诱导严重的叶片坏死和幼苗死亡,而缺乏这些结构的分离株则不致病。这项研究首次在全球发现了 A. gallica 导致猕猴桃根腐病的实例。这一发现以及 A. mellea 在该国的病原鉴定,凸显了根腐病对该地区猕猴桃生产的新威胁。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Armillaria Species Causing Kiwifruit Vine Decline and Root Rot in Northeastern Türkiye: A Growing Concern for Kiwifruit Health

Kiwifruit (Actinidia deliciosa) production in northeastern Türkiye is facing an emerging threat from Armillaria root rot. Surveys conducted in August 2021 and 2022 revealed that 10.5%–17.5% of the 200–500 vines within each of 35 kiwifruit orchards in the Perşembe, Gülyalı and Altınordu districts of Ordu province exhibited symptoms of Armillaria root rot, including complete defoliation. Diseased plants exhibited diagnostic signs on their root collars and woody roots, including white mycelial fans, extensive wood rot and rhizomorphs. A single Armillaria isolate was obtained from each of the 35 symptomatic orchards. Molecular identification, employing DNA sequencing of the large subunit (LSU) region of rDNA, the translation elongation factor subunit 1-alpha (TEF1) gene and the second largest RNA polymerase II B-subunit (RPB2) gene, identified 20 isolates as Armillaria gallica and 15 as A. mellea. Phylogenetic analyses, based on TEF1 alignments, further supported the species identification with a 100% bootstrap value. To examine genetic diversity, start codon targeted (SCoT) marker 13 was used, successfully differentiating the two Armillaria species and revealing three distinct clades. Clades I and II comprised 15 and 5 isolates of A. gallica, respectively, while Clade III contained all 15 A. mellea isolates. Pathogenicity testing on kiwifruit seedlings (cv. Hayward) revealed the ability of both A. gallica and A. mellea isolates to induce disease. Rhizomorph-producing isolates in kiwifruit seedlings displayed high virulence, inducing severe leaf necrosis and seedling mortality, while isolates lacking these structures were non-pathogenic. This study identifies the first global instance of A. gallica causing kiwifruit root rot. This finding, along with the identification of A. mellea as a causal agent in the country, highlights the emerging threat of Armillaria root rot to kiwifruit production in the region.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Phytopathology
Journal of Phytopathology 生物-植物科学
CiteScore
2.90
自引率
0.00%
发文量
88
审稿时长
4-8 weeks
期刊介绍: Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays. Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes. Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.
期刊最新文献
Characterisation of the Phenotypic Reaction of Brazilian Soybean Genotypes to Sclerotinia sclerotiorum Under Controlled Conditions Spark Architecture and Ensemble-Based Feature Selection With Hybrid Optimisation Enabled Deep Long Short-Term Memory for Crop Yield Prediction Pathogenic Variability and Race Structure of Colletotrichum lindemuthianum Isolates From Common Bean in Ethiopia Identification of QoI-Resistant Isolates of the Banana Pathogen Pseudocercospora fijiensis in Mexico In Situ Diagnosis and Digital Cataloguing of Plant Pathogenic Fungi Through Mobile-Based Foldscope Microscopy
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1