Rachiplusia nu（鳞翅目：夜蛾科）对 Cry1Ac 抗性的遗传基础：遗传模式、交叉抗性模式和适应成本

IF 3.8 1区农林科学 Q1 AGRONOMY Pest Management Science Pub Date : 2024-10-15 DOI:10.1002/ps.8475

Alexandre C Reis, Eduardo A Steinhaus, Daniela N Godoy, Luiz F Warpechowski, Luis H M Diniz, Arthur Dallanora, Renato J Horikoshi, Ramiro F L Ovejero, Samuel Martinelli, Geraldo U Berger, Graham P Head, Patrick M Dourado, Oderlei Bernardi

{"title":"Rachiplusia nu（鳞翅目：夜蛾科）对 Cry1Ac 抗性的遗传基础：遗传模式、交叉抗性模式和适应成本","authors":"Alexandre C Reis, Eduardo A Steinhaus, Daniela N Godoy, Luiz F Warpechowski, Luis H M Diniz, Arthur Dallanora, Renato J Horikoshi, Ramiro F L Ovejero, Samuel Martinelli, Geraldo U Berger, Graham P Head, Patrick M Dourado, Oderlei Bernardi","doi":"10.1002/ps.8475","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> BACKGROUND</h3>\n \n <p><i>Rachiplusia nu</i> (Guenée) was historically a secondary soybean pest in Brazil, but a key soybean pest in Argentina. From 2021 onwards, injury caused by <i>R. nu</i> has been reported in soybean that expresses the Cry1Ac toxin from <i>Bacillus thuringiensis</i> (Berliner) in both countries. In this study, we selected resistant and susceptible strains of <i>R. nu</i> to Cry1Ac using Cry1Ac-containing leaf tissue and characterized the inheritance of resistance, cross-resistance patterns and fitness cost.</p>\n </section>\n \n <section>\n \n <h3> RESULTS</h3>\n \n <p>Neonates of the Cry1Ac-resistant strain of <i>R. nu</i> were able to develop on Cry1Ac soybean leaves and emerge as fertile adults, while neonates from the susceptible and heterozygous strains did not survive beyond 10 days. The resistance ratio to Cry1Ac estimated in diet-overlay bioassays in the resistant strain was > 736.92-fold. The inheritance pattern of Cry1Ac resistance in <i>R. nu</i> was characterized as autosomal recessive and monogenic. The Cry1Ac-resistant strain of <i>R. nu</i> also exhibited high resistance to Cry1A.105 (resistance ratio > 159.87-fold), but negligible resistance to Cry2Ab2 (resistance ratio = 1.25-fold). Life history data showed that the resistance to Cry1Ac in <i>R. nu</i> is not associated with a substantial fitness cost.</p>\n </section>\n \n <section>\n \n <h3> CONCLUSIONS</h3>\n \n <p>The inheritance pattern of Cry1Ac resistance in <i>R. nu</i> is autosomal recessive, monogenic and not associated with obvious fitness costs. Cross-resistance occurred between Cry1Ac and Cry1A.105 in <i>R. nu</i> but not between Cry1Ac and Cry2Ab2, indicating that Cry1A.105/Cry2Ab2/Cry1Ac soybean is a valuable tool to manage Cry1Ac resistance in <i>R. nu</i>. This is the first study reporting the genetic basis of Cry1Ac resistance in <i>R. nu</i>. © 2024 Society of Chemical Industry.</p>\n </section>\n </div>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"81 2","pages":"727-735"},"PeriodicalIF":3.8000,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genetic basis of resistance to Cry1Ac in Rachiplusia nu (Lepidoptera: Noctuidae): inheritance mode, cross-resistance patterns and fitness cost\",\"authors\":\"Alexandre C Reis, Eduardo A Steinhaus, Daniela N Godoy, Luiz F Warpechowski, Luis H M Diniz, Arthur Dallanora, Renato J Horikoshi, Ramiro F L Ovejero, Samuel Martinelli, Geraldo U Berger, Graham P Head, Patrick M Dourado, Oderlei Bernardi\",\"doi\":\"10.1002/ps.8475\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> BACKGROUND</h3>\\n \\n <p><i>Rachiplusia nu</i> (Guenée) was historically a secondary soybean pest in Brazil, but a key soybean pest in Argentina. From 2021 onwards, injury caused by <i>R. nu</i> has been reported in soybean that expresses the Cry1Ac toxin from <i>Bacillus thuringiensis</i> (Berliner) in both countries. In this study, we selected resistant and susceptible strains of <i>R. nu</i> to Cry1Ac using Cry1Ac-containing leaf tissue and characterized the inheritance of resistance, cross-resistance patterns and fitness cost.</p>\\n </section>\\n \\n <section>\\n \\n <h3> RESULTS</h3>\\n \\n <p>Neonates of the Cry1Ac-resistant strain of <i>R. nu</i> were able to develop on Cry1Ac soybean leaves and emerge as fertile adults, while neonates from the susceptible and heterozygous strains did not survive beyond 10 days. The resistance ratio to Cry1Ac estimated in diet-overlay bioassays in the resistant strain was > 736.92-fold. The inheritance pattern of Cry1Ac resistance in <i>R. nu</i> was characterized as autosomal recessive and monogenic. The Cry1Ac-resistant strain of <i>R. nu</i> also exhibited high resistance to Cry1A.105 (resistance ratio > 159.87-fold), but negligible resistance to Cry2Ab2 (resistance ratio = 1.25-fold). Life history data showed that the resistance to Cry1Ac in <i>R. nu</i> is not associated with a substantial fitness cost.</p>\\n </section>\\n \\n <section>\\n \\n <h3> CONCLUSIONS</h3>\\n \\n <p>The inheritance pattern of Cry1Ac resistance in <i>R. nu</i> is autosomal recessive, monogenic and not associated with obvious fitness costs. Cross-resistance occurred between Cry1Ac and Cry1A.105 in <i>R. nu</i> but not between Cry1Ac and Cry2Ab2, indicating that Cry1A.105/Cry2Ab2/Cry1Ac soybean is a valuable tool to manage Cry1Ac resistance in <i>R. nu</i>. This is the first study reporting the genetic basis of Cry1Ac resistance in <i>R. nu</i>. © 2024 Society of Chemical Industry.</p>\\n </section>\\n </div>\",\"PeriodicalId\":218,\"journal\":{\"name\":\"Pest Management Science\",\"volume\":\"81 2\",\"pages\":\"727-735\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2024-10-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pest Management Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.8475\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pest Management Science","FirstCategoryId":"97","ListUrlMain":"https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.8475","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

摘要

Rachiplusia nu（Guenée）历来是巴西大豆的次要害虫，但却是阿根廷大豆的主要害虫。从 2021 年起，两国都有关于 R. nu 对表达苏云金芽孢杆菌（柏林根）Cry1Ac 毒素的大豆造成伤害的报道。在本研究中，我们利用含 Cry1Ac 的叶片组织筛选出对 Cry1Ac 有抗性和易感性的 R. nu 株系，并描述了抗性遗传、交叉抗性模式和适应成本。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

摘要图片

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

Genetic basis of resistance to Cry1Ac in Rachiplusia nu (Lepidoptera: Noctuidae): inheritance mode, cross-resistance patterns and fitness cost

BACKGROUND

Rachiplusia nu (Guenée) was historically a secondary soybean pest in Brazil, but a key soybean pest in Argentina. From 2021 onwards, injury caused by R. nu has been reported in soybean that expresses the Cry1Ac toxin from Bacillus thuringiensis (Berliner) in both countries. In this study, we selected resistant and susceptible strains of R. nu to Cry1Ac using Cry1Ac-containing leaf tissue and characterized the inheritance of resistance, cross-resistance patterns and fitness cost.

RESULTS

Neonates of the Cry1Ac-resistant strain of R. nu were able to develop on Cry1Ac soybean leaves and emerge as fertile adults, while neonates from the susceptible and heterozygous strains did not survive beyond 10 days. The resistance ratio to Cry1Ac estimated in diet-overlay bioassays in the resistant strain was > 736.92-fold. The inheritance pattern of Cry1Ac resistance in R. nu was characterized as autosomal recessive and monogenic. The Cry1Ac-resistant strain of R. nu also exhibited high resistance to Cry1A.105 (resistance ratio > 159.87-fold), but negligible resistance to Cry2Ab2 (resistance ratio = 1.25-fold). Life history data showed that the resistance to Cry1Ac in R. nu is not associated with a substantial fitness cost.

CONCLUSIONS

The inheritance pattern of Cry1Ac resistance in R. nu is autosomal recessive, monogenic and not associated with obvious fitness costs. Cross-resistance occurred between Cry1Ac and Cry1A.105 in R. nu but not between Cry1Ac and Cry2Ab2, indicating that Cry1A.105/Cry2Ab2/Cry1Ac soybean is a valuable tool to manage Cry1Ac resistance in R. nu. This is the first study reporting the genetic basis of Cry1Ac resistance in R. nu. © 2024 Society of Chemical Industry.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.