新发急性髓性白血病患者中 SNHG7 和 BGL3 表达的相关性;关于 lncRNA 在急性髓性白血病发病机制中 PI3K 信号转导背景下的作用的新见解

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemistry and Biophysics Reports Pub Date : 2024-10-18 DOI:10.1016/j.bbrep.2024.101850
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引用次数: 0

摘要

背景由于急性髓性白血病(AML)的异质性,以及为这种恶性肿瘤寻找合适治疗策略的障碍,发现一种可靠的生物标志物以改善治疗和预测患者预后已被确定为当务之急。考虑到长非编码RNA(lncRNA)SNHG7和BGL3在各种癌症中的参与,这些lncRNA的确切表达模式及其在急性髓性白血病(AML)中的临床意义仍然难以捉摸。为了证明急性髓性白血病发病的可能机制,我们的目标是检测 BGL3 和 SNHG7 lncRNA 在 PI3K 通路中的表达。采用 qRT-PCR 技术测定了 SNHG7 和 BGL3 lncRNA 及其靶基因 Akt 和 PTEN 的表达水平。结果在 AML 样本中,SNHG7 lncRNA 和 Akr 基因的表达水平显著升高,而 BGL3 lncRNA 和 PTEN 基因的表达水平明显降低。不过,上述基因/lncRNA的表达水平与年龄和性别之间并无明显关系。结论作为对研究结果最直观的解释,我们认为 SNHG7 和 BGL3 通过 Akt 和 PTEN 之间的相互作用而建立的关联可能在急性髓细胞性白血病的发病机制中起着至关重要的作用,而这一轴心的任何元素都可能成为进一步深入治疗策略的潜在新靶点。然而,对于血液恶性肿瘤,尤其是急性髓细胞性白血病,还需要在这一领域进行更详细的研究,以阐明这一有趣的睾丸特异性通路所发挥的确切作用。
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Correlation of SNHG7 and BGL3 expression in patients with de novo acute myeloid leukemia; novel insights into lncRNA effect in PI3K signaling context in AML pathogenesis

Background

Acute myeloid leukemia (AML) has been identified as a top priority for discovering a reliable biomarker for treatment improvement and patient outcome prediction due to the heterogeneous nature of AML and the obstacle to find an appropriate treatment strategy for this malignancy. Considering the involvement of long noncoding RNA (lncRNA) SNHG7 and BGL3 found in various cancers, the exact expression pattern of these lncRNAs and their clinical implications in acute myeloid leukemia (AML) continue to be elusive. In order to demonstrate a possible mechanism underlying AML pathogenesis, our goal was to examine BGL3 and SNHG7 lncRNA expressions in PI3K pathway.

Methods

This case-control cross-sectional study were conducted on RNA extracted from blood samples of 30 patients diagnosed with AML (Ayatollah-Khansari hospital, Arak, Iran) and 30 (age and gender matched) healthy controls. The expression levels of SNHG7 and BGL3 lncRNAs and their target genes Akt and PTEN, were measured using qRT-PCR. Subsequently, by means of statistical analysis, we determined the plausible correlation between the expressions of the aforementioned genes and lncRNA respectively.

Results

In AML samples, a considerable increase in the expression levels of SNHG7 lncRNA and Akr gene was accompanied by a marked reduction in the expression levels of BGL3 lncRNA and PTEN gene. Nevertheless, No significant relationship between the expression level of the indicated genes/lncRNAs and age and sex was found. The remarkable correlation between the expression of genes/lncRNAs and the blast percentage in patients was the notable point in the result of this study.

Conclusions

As the most straightforward interpretation of our results, we propose that perhaps the association between SNHG7 and BGL3 built through the interaction between Akt and PTEN may play a crucial role in the AML pathogenesis and any element of this axis could be a potential novel target for further profound treatment strategies. Nonetheless, in the context of Hematological Malignancies, particularly AML, more detailed studies are needed in this area to elucidate the precise role played by this interesting testis-specific pathway.
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来源期刊
Biochemistry and Biophysics Reports
Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
4.60
自引率
0.00%
发文量
191
审稿时长
59 days
期刊介绍: Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.
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