快速筛选冬小麦幼苗对 Sitobion avenae (F.) 和 Rhopalosiphum padi (L.) 的抗性并选择高效的评估方法

IF 3.8 1区农林科学 Q1 AGRONOMY Pest Management Science Pub Date : 2024-10-18 DOI:10.1002/ps.8485

Ilma A Qonaah, Amma L Simon, Duncan Warner, Rosanna M Rostron, Toby J A Bruce, Rumiana V Ray

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引用次数: 0

摘要

Sitobion avenae (F.) 和 Rhopalosiphum padi (L.) 是小麦 [Triticum aestivum (L.)] 的有害害虫。目前尚未在商业小麦品种中发现抗蚜虫的基因，而抗性表型分析费时费力。在此，我们测试了一种高通量表型方法，用于筛选 29 个冬小麦商品品种的蚜虫抗性和抗药性。我们利用综合行为分析验证了这种方法，包括褐飞虱对挥发性有机化合物（VOCs）的吸引力和利用电穿透图（EPG）进行的摄食生物测定，随后突出了可能的抗性来源。

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Rapid screening for resistance to Sitobion avenae (F.) and Rhopalosiphum padi (L.) in winter wheat seedlings and selection of efficient assessment methods

BACKGROUND

Sitobion avenae (F.) and Rhopalosiphum padi (L.) are harmful pests of wheat [Triticum aestivum (L.)]. No genetic resistance against the aphids has been identified in commercial wheat varieties and resistance phenotyping can be time-consuming and laborious. Here, we tested a high-throughput phenotyping method to screen 29 commercial winter wheat varieties for alate antixenosis and antibiosis. We validated this method using comprehensive behavioural analyses, including alate attraction to volatile organic compounds (VOCs) and a feeding bioassay using an electrical penetration graph (EPG), subsequently highlighting possible sources of resistance.

RESULTS

We observed differences in alate behaviour upon assessing alate settlement on wheat seedlings and attraction towards VOCs, revealing the importance of visual and early post-alighting cues for alate host selection. Aphid settlement was four times higher on the most preferred variety than on the least preferred variety. Using an EPG bioassay, we identified phloem feeding and stylet derailment parameters linked to resistance. We found antibiosis assessment on detached leaves to be an inadequate screen because it produced results inconsistent with intact leaves assessment. Alate and nymph mortality were identified as key traits signifying antibiosis, showing significant positive relationships with alate reproduction and nymph mean relative growth rate.

CONCLUSIONS

Overall, antixenosis and antibiosis varietal responses were consistent for both aphid species. Alate settlement on wheat seedlings was a more efficient antixenosis screen than an olfactometer assay using VOCs. In addition to assessing alate and nymph survival for antibiosis, this allows for more rapid phenotyping of large numbers of genotypes to identify novel aphid resistance genes for varietal improvement. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.