Benjamin Schmuck, Gabriele Greco, Olga Shilkova, Anna Rising
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引用次数: 0
摘要
蜘蛛可以生产多达七种不同类型的丝,每种丝都具有独特的机械特性,这些特性源于蛛丝蛋白(spidroins)重复区域的变化。人工蛛丝可通过全水基纺丝工艺由小蜘蛛丝蛋白制成,但最强的纤维强度很少超过原生丝纤维的 25%。为了改善用迷你蜘蛛蛋白制成的蚕丝纤维的机械性能,并了解蛋白质设计与纤维机械性能之间的关系,我们设计了 16 种新的蜘蛛蛋白,它们的分子量从 31.7 到 59.5 kDa 不等,具有球状蜘蛛蛋白 N 端和 C 端结构域,但含有不同的重复序列。我们发现,将这些构建体挤入低pH值的水缓冲液中,50%以上的构建体可以纺成纤维,而重复区域来自主要安普拉蝶呤4(MaSp4)和弹性蛋白的蛋白质所制成的纤维最好。不同类型纤维的机械性能各不相同,但与基于重复区来源的预期性能并不相关,这表明除蛋白质设计外,还有其他因素影响着纤维的性能。
Effects of Mini-Spidroin Repeat Region on the Mechanical Properties of Artificial Spider Silk Fibers.
Spiders can produce up to seven different types of silk, each with unique mechanical properties that stem from variations in the repetitive regions of spider silk proteins (spidroins). Artificial spider silk can be made from mini-spidroins in an all-aqueous-based spinning process, but the strongest fibers seldom reach more than 25% of the strength of native silk fibers. With the aim to improve the mechanical properties of silk fibers made from mini-spidroins and to understand the relationship between the protein design and the mechanical properties of the fibers, we designed 16 new spidroins, ranging from 31.7 to 59.5 kDa, that feature the globular spidroin N- and C-terminal domains, but harbor different repetitive sequences. We found that more than 50% of these constructs could be spun by extruding them into low-pH aqueous buffer and that the best fibers were produced from proteins whose repeat regions were derived from major ampullate spidroin 4 (MaSp4) and elastin. The mechanical properties differed between fiber types but did not correlate with the expected properties based on the origin of the repeats, suggesting that additional factors beyond protein design impact the properties of the fibers.
ACS OmegaChemical Engineering-General Chemical Engineering
CiteScore
6.60
自引率
4.90%
发文量
3945
审稿时长
2.4 months
期刊介绍:
ACS Omega is an open-access global publication for scientific articles that describe new findings in chemistry and interfacing areas of science, without any perceived evaluation of immediate impact.